BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0225.p (554 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 486 e-138 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 394 e-110 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 369 e-103 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 337 2e-93 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 283 2e-77 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 486 bits (1252), Expect = e-138, Method: Compositional matrix adjust. Identities = 278/453 (61%), Positives = 337/453 (74%), Gaps = 11/453 (2%) Query: 16 GCVSLAPDYIRPALPVPTHYP----NAAA---ALRESAEPIGWRSYFQDAHLQSLIELAL 68 C SLAP Y RPA PV YP N AA A ++ P+ WR YF D LQ+LI AL Sbjct: 24 ACQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQAL 83 Query: 69 QNSQDLRLALARVDEARAAYGIEAAAQFPTVGAQAGLDRARVPGDLNLTGKPLVSSQYQV 128 +NS+DLR A RV EA+A YGI A FPT+GAQAGLDR+R P DLNLT +PLV S YQ Sbjct: 84 ENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQA 143 Query: 129 GLGMAGWELDFWGRVRSLKDAALESYLASDAARRAVRIDLIAQVAITYLTLSELNERLFL 188 GLG++ WE+D WGR+RSL DAALES AS A R AV + L+AQVA +L L+EL+ERL + Sbjct: 144 GLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEI 203 Query: 189 AGQTLASRAESYRIFKRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQEREMQIHRLALL 248 A QT+ASR ES+RIF RRVEVG+TSRLNLTQ+ LLTQAQ+L AQL+Q+R +++ L LL Sbjct: 204 ANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLL 263 Query: 249 VGTQLSEATLQQR--LSEVEAPRRLRPGIPSDLLNNRPDIIAAEHQLRAANANIGAARAA 306 VG+ + Q R L V AP L+PG+P+ LL RPD+ AAEH+L AANANIGAARAA Sbjct: 264 VGSPVVLPVGQARAGLDAVFAP--LQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAA 321 Query: 307 FFPRIALTGSAGTASAELGGLFDSGSRAWTFSPGISLPLFDGGLRNSNLKLTEARRHIAV 366 FFP I+LTG+ GTASAEL GLF GS AWTFSP I+LP+F G +NL L+E RR +AV Sbjct: 322 FFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAV 381 Query: 367 LNYEKAIQSAFRDVSDALSARVWLDYQVDIAAVTLEVQAERARLSNLRYDNGSASFLEVL 426 YEK +Q+AFR+V+DAL+AR WL Q++IA + VQ ERARLS LRYDNG+A FL+VL Sbjct: 382 AAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVL 441 Query: 427 DAQRDLLVAQQQLVQLQRALAAGNVSLYAALGG 459 DAQRDLL AQQQLVQ++RAL + +V+LYAALGG Sbjct: 442 DAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 394 bits (1011), Expect = e-110, Method: Compositional matrix adjust. Identities = 224/465 (48%), Positives = 302/465 (64%), Gaps = 6/465 (1%) Query: 4 RLIMALAALQ----LGGCVSLAPDYIRPALPVPTHYPNAAAALRE-SAEPIGWRSYFQDA 58 RL+ AAL LGGC SLAP Y RP PV + YP AA E A +GWR +F D Sbjct: 11 RLLHMCAALVMAGVLGGC-SLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQ 69 Query: 59 HLQSLIELALQNSQDLRLALARVDEARAAYGIEAAAQFPTVGAQAGLDRARVPGDLNLTG 118 LQ LI AL+N++DLR A+ ++EARA Y I A P + R R +LTG Sbjct: 70 RLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTG 129 Query: 119 KPLVSSQYQVGLGMAGWELDFWGRVRSLKDAALESYLASDAARRAVRIDLIAQVAITYLT 178 +P +S+ YQVGL + +ELDF+GRVR+LKDAAL SYL+++ A+++ +I L+AQVA YL Sbjct: 130 QPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLA 189 Query: 179 LSELNERLFLAGQTLASRAESYRIFKRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQER 238 E+L LA +TL SR + + ++R++VG+++ L+ L+ A+ QLE++R Sbjct: 190 ERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQR 249 Query: 239 EMQIHRLALLVGTQLSEATLQQRLSEVEAPRRLRPGIPSDLLNNRPDIIAAEHQLRAANA 298 + L LLVG ++ E Q LS+ + + G+PSDLL NRPDI +AE L AANA Sbjct: 250 AQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANA 309 Query: 299 NIGAARAAFFPRIALTGSAGTASAELGGLFDSGSRAWTFSPGISLPLFDGGLRNSNLKLT 358 NIGAARAAFFPRI+LTG+ G+AS+ LGGLF+SGS AW+F P +SLP+FD G ++NL L Sbjct: 310 NIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLA 369 Query: 359 EARRHIAVLNYEKAIQSAFRDVSDALSARVWLDYQVDIAAVTLEVQAERARLSNLRYDNG 418 E R++ AV+ YEKAIQ+AFR+V+DAL AR LD QV L Q ER +L++LR+ NG Sbjct: 370 EVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNG 429 Query: 419 SASFLEVLDAQRDLLVAQQQLVQLQRALAAGNVSLYAALGGAADD 463 AS L+ LDAQRDL AQQ L+Q ++ + LY ALGG ++ Sbjct: 430 IASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNE 474 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 369 bits (946), Expect = e-103, Method: Compositional matrix adjust. Identities = 219/473 (46%), Positives = 292/473 (61%), Gaps = 26/473 (5%) Query: 5 LIMALAALQLGGCVSLAPDYIRPALPVPTHY-----PNAAAALRESAEP----IGWRSYF 55 ++ + A+ LGGC +L PDY RP PV H+ NAA A S+ P IGWR F Sbjct: 15 MLPVMLAMLLGGC-TLMPDYHRPDAPVAAHFAGADSKNAAGA---SSAPVVADIGWRDVF 70 Query: 56 QDAHLQSLIELALQNSQDLRLALARVDEARAAYGIEAAAQFPTVGAQAGLDRARVPGDLN 115 D LQ +I LAL+N++DLR+A+ +++ARA YG++ AA FP++ A +R PGDL+ Sbjct: 71 TDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLS 130 Query: 116 LTGKPLVSSQYQVGLGMAGWELDFWGRVRSLKDAALESYLASDAARRAVRIDLIAQVAIT 175 TG L S Y LG + +ELD +GRVRSLK+ AL+S+L++ ARR+ +I LIA+VA Sbjct: 131 STGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATA 190 Query: 176 YLTLSELNERLFLAGQTLASRAESYRIFKRRVEVGSTSRLNLTQVAVLLTQA----QSLV 231 +LTL+ +RL LA TL S++ SY + +R E+GS+S L L Q + A +S Sbjct: 191 WLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYT 250 Query: 232 AQLEQEREMQIHRLALLVGTQLSEATLQQ-----RLSEVEAPRRLRPGIPSDLLNNRPDI 286 AQ++Q+R + L LLVG L A L Q RL+ + PG+PSDLL R DI Sbjct: 251 AQVDQDR----NALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADI 306 Query: 287 IAAEHQLRAANANIGAARAAFFPRIALTGSAGTASAELGGLFDSGSRAWTFSPGISLPLF 346 + AE LRAANANIGAARAAF+P I LT +AGTASA L GLF GS +W+F P ISLP+F Sbjct: 307 VQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIF 366 Query: 347 DGGLRNSNLKLTEARRHIAVLNYEKAIQSAFRDVSDALSARVWLDYQVDIAAVTLEVQAE 406 DGG +NL + R I+V YEKAIQ+AFR+VSDAL+ R L Q+ ++ E Sbjct: 367 DGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDE 426 Query: 407 RARLSNLRYDNGSASFLEVLDAQRDLLVAQQQLVQLQRALAAGNVSLYAALGG 459 RLS RY +G S+L VLD+QR L AQ+ L+ Q + V+ Y LGG Sbjct: 427 AFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGG 479 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 337 bits (863), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 191/456 (41%), Positives = 278/456 (60%), Gaps = 12/456 (2%) Query: 16 GCVSLAPDYIRPALPVPTHYPNAAAAL-----------RESAEPIGWRSYFQDAHLQSLI 64 GC +LAPDY +PA PVP + + AAA A+ +GWR +F D LQ +I Sbjct: 27 GCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVI 86 Query: 65 ELALQNSQDLRLALARVDEARAAYGIEAAAQFPTVGAQAGLDRARVPGDLNLTGKPLVSS 124 AL N++DLR+A +++ARA Y I A ++P++ A +R P +L+ TG VS Sbjct: 87 AAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSH 146 Query: 125 QYQVGLGMAGWELDFWGRVRSLKDAALESYLASDAARRAVRIDLIAQVAITYLTLSELNE 184 Q V +G++ +ELDF+GR+++L DAALESY RR+ I L+A+VA +LTL+ + Sbjct: 147 QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRD 206 Query: 185 RLFLAGQTLASRAESYRIFKRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQEREMQIHR 244 L LA T AS++ ++ + +R ++G+ S ++L + A++ VA+ + E ++ Sbjct: 207 LLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNA 266 Query: 245 LALLVGTQLSEATLQQRLSEVEAPRRLRP-GIPSDLLNNRPDIIAAEHQLRAANANIGAA 303 L LLVG + A L L + + P G+PS +L RPD++AAEH L++ANA+IGAA Sbjct: 267 LRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAA 326 Query: 304 RAAFFPRIALTGSAGTASAELGGLFDSGSRAWTFSPGISLPLFDGGLRNSNLKLTEARRH 363 RAAFFP I+LT + GTAS LGGLF +GS +WTFSP I+LP+F+ G ++L + R Sbjct: 327 RAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRD 386 Query: 364 IAVLNYEKAIQSAFRDVSDALSARVWLDYQVDIAAVTLEVQAERARLSNLRYDNGSASFL 423 I V NYEKAIQ+AFR+V+DAL+ R LD ++D + RLS+ RY NG S+L Sbjct: 387 IDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYL 446 Query: 424 EVLDAQRDLLVAQQQLVQLQRALAAGNVSLYAALGG 459 L AQR L AQQ L+ L+ + ++LY LGG Sbjct: 447 TTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGG 482 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 283 bits (724), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 194/473 (41%), Positives = 270/473 (57%), Gaps = 5/473 (1%) Query: 3 SRLIMALAALQLGGCVSLAPDYIRPALPVPTHYP-----NAAAALRESAEPIGWRSYFQD 57 SR + L L L C L AL +PTHYP +A A +A IGW+ YF Sbjct: 6 SRHLRLLPTLALCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFPQ 65 Query: 58 AHLQSLIELALQNSQDLRLALARVDEARAAYGIEAAAQFPTVGAQAGLDRARVPGDLNLT 117 A LQ+LI AL+++ DLR R+ + +A + I+ + PTV +A R+R P + + Sbjct: 66 APLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPES 125 Query: 118 GKPLVSSQYQVGLGMAGWELDFWGRVRSLKDAALESYLASDAARRAVRIDLIAQVAITYL 177 G + +Y+VGLG + WELDFWGR+ SL +AAL+ ++AS AA +A+ I LI QVA + Sbjct: 126 GPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWT 185 Query: 178 TLSELNERLFLAGQTLASRAESYRIFKRRVEVGSTSRLNLTQVAVLLTQAQSLVAQLEQE 237 +L EL+ERL L A+ AES + + R + G L Q L Q ++ A+L+Q Sbjct: 186 SLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQM 245 Query: 238 REMQIHRLALLVGTQLSEATLQQRLSEVEAPRRLRPGIPSDLLNNRPDIIAAEHQLRAAN 297 R Q L +L G A L+ + + P L PG+PS LL RPDIIAAEH+L+A+ Sbjct: 246 RASQRSTLEMLAGLTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQASA 305 Query: 298 ANIGAARAAFFPRIALTGSAGTASAELGGLFDSGSRAWTFSPGISLPLFDGGLRNSNLKL 357 A + AARAAFFPRI+LT G AS L LF SRAW F P ISLPL DGGLR S + Sbjct: 306 AQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDI 365 Query: 358 TEARRHIAVLNYEKAIQSAFRDVSDALSARVWLDYQVDIAAVTLEVQAERARLSNLRYDN 417 + A+L+YE+ +Q+AFR+V+ L WL Q+ + ++ QA+R ++R Sbjct: 366 AALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRY 425 Query: 418 GSASFLEVLDAQRDLLVAQQQLVQLQRALAAGNVSLYAALGGAADDAAAGXSS 470 G AS L+VL+++RD L AQQ+ L+ AL ++LY ALGG +A S Sbjct: 426 GRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPS 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,277,271 Number of extensions: 45652 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 19 Length of query: 554 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 464 Effective length of database: 1,193,720 Effective search space: 553886080 Effective search space used: 553886080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)