BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0222.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPas... 427 e-121 gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPas... 341 3e-95 gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate A... 92 7e-20 gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transport... 90 2e-19 gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPas... 90 2e-19 >gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 923 Score = 427 bits (1099), Expect = e-121, Method: Compositional matrix adjust. Identities = 205/260 (78%), Positives = 229/260 (88%) Query: 18 TGVDPLSRAQFWDLIGKIRGERAGMSVIVATAYMEEADRFDWLVAMDDGKILATGTPREL 77 TGVDPLSRAQFWDLI IR +RAGMSVIVATAYMEEADRFDWLVAMD+G++LATGTP +L Sbjct: 174 TGVDPLSRAQFWDLIAHIRQQRAGMSVIVATAYMEEADRFDWLVAMDEGRVLATGTPVQL 233 Query: 78 HERVGAASLEAAFIAMLPEAKRRNHRAVVVTPLQPSDNEETAIEAKGLTMRFGDFVAVAH 137 H+R G ASLE AFIA+LPE KRR H+ V VTPL + + AIEAKGLTMRFGDF+AV H Sbjct: 234 HQRTGTASLEEAFIALLPEEKRRGHQTVQVTPLDDTIDSGVAIEAKGLTMRFGDFLAVDH 293 Query: 138 VDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWLFGRKVDGSDITTRQRVGYM 197 VDF+IRRGEIFGFLGSNGCGKSTTMKMLTGL+PASEG+AWLFGR++D D+ TR RVGYM Sbjct: 294 VDFRIRRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGQAWLFGREIDPRDMATRARVGYM 353 Query: 198 SQAFSLYGELTVRQNLVLHARLFQVPESAIAARVDEMAQRFDLRQVLDKLPEGLPLGVRQ 257 SQAFSLYGEL+VRQNLVLHARL++VP IAAR DEMA+RF L V+D LPE LPLG+RQ Sbjct: 354 SQAFSLYGELSVRQNLVLHARLYRVPAEQIAARTDEMARRFGLLDVMDSLPESLPLGIRQ 413 Query: 258 RLSLAVAMVHRPELLILDEP 277 RLSLAVAMVH+PELLILDEP Sbjct: 414 RLSLAVAMVHKPELLILDEP 433 Score = 78.2 bits (191), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 52/159 (32%), Positives = 81/159 (50%), Gaps = 8/159 (5%) Query: 125 LTMRFGDFVAVAHVDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWLFGRKVD 184 + +R+G VA+ V I G + G +G +G GKS+ + ++ G +G+ + G D Sbjct: 16 VALRYGKTVALDAVSLDIPAGCMVGLIGPDGVGKSSLLSLVAGARALQQGELAVLGG--D 73 Query: 185 GSDITTRQ----RVGYMSQAF--SLYGELTVRQNLVLHARLFQVPESAIAARVDEMAQRF 238 RQ R+ YM Q +LY L+V +NL RLF S R+DE+ Sbjct: 74 MRQQRHRQQACPRIAYMPQGLGKNLYPTLSVEENLQFFGRLFGQDASERRRRIDELTTST 133 Query: 239 DLRQVLDKLPEGLPLGVRQRLSLAVAMVHRPELLILDEP 277 L + L + L G++Q+L L +++H P+LLILDEP Sbjct: 134 GLDKFLARPAGKLSGGMKQKLGLCCSLIHDPDLLILDEP 172 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 32/80 (40%), Positives = 51/80 (63%), Gaps = 3/80 (3%) Query: 18 TGVDPLSRAQFWDL-IGKIRGERAGMSVIVATAYMEEADRFDWLVAMDDGKILATGTPRE 76 +GVDP++R FW L IG R ++ +++ ++T +M EA R D + M G++L + TP+ Sbjct: 435 SGVDPIARDLFWQLMIGLARQDQ--VTIFISTHFMNEALRCDRISLMHAGRVLVSATPQA 492 Query: 77 LHERVGAASLEAAFIAMLPE 96 L ++ GA +LE AFI L E Sbjct: 493 LMQQRGAQTLEQAFIGYLEE 512 >gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 913 Score = 341 bits (875), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 166/261 (63%), Positives = 205/261 (78%), Gaps = 2/261 (0%) Query: 18 TGVDPLSRAQFWDLIGKIRGERAGMSVIVATAYMEEADRFDWLVAMDDGKILATGTPREL 77 TGVDPL+RAQFW+LI ++R GMSVIVATAYM+EA FDWL MD G++LATGTP L Sbjct: 171 TGVDPLARAQFWELIARLRQRHPGMSVIVATAYMDEAQAFDWLAVMDQGQVLATGTPAAL 230 Query: 78 HERVGAASLEAAFIAMLPEAKRRNHRAVVVTPLQPSDNEETAIEAKGLTMRFGD-FVAVA 136 R GA+SLE AFIA+LP ++ H A+ + PL+ + ++ AIEA+ L+MRF D F AV Sbjct: 231 LARTGASSLERAFIALLPAHRKAGHEALSIPPLE-THADDIAIEAENLSMRFADGFTAVD 289 Query: 137 HVDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWLFGRKVDGSDITTRQRVGY 196 HV F+IRRGEIFGFLGSNGCGK+TTMKMLTGL+PAS G+A LFG+++D D+ R+RVGY Sbjct: 290 HVSFRIRRGEIFGFLGSNGCGKTTTMKMLTGLLPASSGRARLFGQEIDPRDVCLRKRVGY 349 Query: 197 MSQAFSLYGELTVRQNLVLHARLFQVPESAIAARVDEMAQRFDLRQVLDKLPEGLPLGVR 256 MSQ+FSLY +L+VRQNL LHARLF +P + I RV EMA+RF L V+D L LP+G+R Sbjct: 350 MSQSFSLYKDLSVRQNLALHARLFHLPAAQIGPRVAEMARRFALMPVMDSLAADLPMGIR 409 Query: 257 QRLSLAVAMVHRPELLILDEP 277 QRLSLAVA +H PE+LILDEP Sbjct: 410 QRLSLAVATIHGPEILILDEP 430 Score = 77.8 bits (190), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 52/155 (33%), Positives = 79/155 (50%), Gaps = 8/155 (5%) Query: 129 FGDFVAVAHVDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWLFGRKVDGSDI 188 +G A++HV+ I G + G +G +G GKST + ++ G G+ + G + Sbjct: 17 YGKNPALSHVELDIPGGCMAGLIGPDGVGKSTLLSLIAGARRIRAGRIDVLGGDI--GQA 74 Query: 189 TTRQRVG----YMSQAF--SLYGELTVRQNLVLHARLFQVPESAIAARVDEMAQRFDLRQ 242 R VG YM Q +LY L+V +NL ARLF R+D++ L+ Sbjct: 75 AHRDAVGPCIAYMPQGLGKNLYPTLSVEENLQFIARLFGQDAQERRQRIDDLTHSTGLQP 134 Query: 243 VLDKLPEGLPLGVRQRLSLAVAMVHRPELLILDEP 277 L + L G++Q+LSL A++H P+LLILDEP Sbjct: 135 FLARPAGQLSGGMKQKLSLCCALIHDPDLLILDEP 169 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 38/108 (35%), Positives = 58/108 (53%), Gaps = 8/108 (7%) Query: 18 TGVDPLSRAQFWDLIGKIRGERAGMSVIVATAYMEEADRFDWLVAMDDGKILATGTPREL 77 +GVDP++R FW L+ + R +++ ++T +M EA R D + M GK+L + TP L Sbjct: 432 SGVDPVARDHFWQLLVTL-SRRDKVTIFLSTHFMNEALRCDRMSMMHAGKVLDSDTPAAL 490 Query: 78 HERVGAASLEAAFIAMLPEAKRRNHRAVVVTPLQPSDNEETAIEAKGL 125 + GAA+LE AFI L +A + A P + +TA GL Sbjct: 491 MHKRGAATLEQAFIGYLVQAGQDEKHA-------PGQSRQTAAMPPGL 531 >gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 91.7 bits (226), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 55/175 (31%), Positives = 93/175 (53%), Gaps = 9/175 (5%) Query: 108 TPLQPSDNEETAIEAKGLTMRFGD-----FVAVAHVDFKIRRGEIFGFLGSNGCGKSTTM 162 TP + E AIE + ++ RF VA+ + RGE +G GCGKSTT+ Sbjct: 10 TPAGQAAQETPAIELRNVSCRFITADGRATVALRDFSMSVARGEFVAVVGPTGCGKSTTL 69 Query: 163 KMLTGLIPASEGKAWLFGRKVDGSDITTRQRVGYMSQAFSLYGELTVRQNLVLHARLFQV 222 ++TGL+ + G+ + G+ V+G D R+G++ Q +++ +VR+N+ Sbjct: 70 SLITGLLKPTLGEVRVMGQPVNGID----PRIGFVFQNDAVFPWRSVRENVAAGPLFRGQ 125 Query: 223 PESAIAARVDEMAQRFDLRQVLDKLPEGLPLGVRQRLSLAVAMVHRPELLILDEP 277 P++ A DE +R L + D P L G+R+R++LA ++ PE+L++DEP Sbjct: 126 PKAQAHALADEWIRRVGLDKFGDHYPHQLSGGMRKRVALAQTFINSPEILLMDEP 180 >gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 54/163 (33%), Positives = 86/163 (52%), Gaps = 5/163 (3%) Query: 119 AIEAKGLTMRFGDFVAVAHVDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWL 178 +IE + RFG F A+ V GE+ LG +GCGK+T ++++ GL G+ L Sbjct: 2 SIEVHHIQKRFGSFTALNDVSLNFPAGELTALLGPSGCGKTTLLRIIAGLESPDSGQVLL 61 Query: 179 FGRKVDGSDITTRQRVGYMSQAFSLYGELTVRQNLVLHARL---FQVP-ESAIAARVDEM 234 G + RQ VG++ Q ++L+ +TV +N+ R+ Q P E+ I +V + Sbjct: 62 DGEDASARHVRERQ-VGFVFQHYALFKHMTVFENIAFGLRVKPRSQRPSEAVIREKVKRL 120 Query: 235 AQRFDLRQVLDKLPEGLPLGVRQRLSLAVAMVHRPELLILDEP 277 + L + D+ P L G RQR++LA A+ P +L+LDEP Sbjct: 121 LELVQLDWLADRYPPQLSGGQRQRIALARALAVEPRVLLLDEP 163 >gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 56/166 (33%), Positives = 91/166 (54%), Gaps = 14/166 (8%) Query: 120 IEAKGLTMRFGDFVAVAHVDFKIRRGEIFGFLGSNGCGKSTTMKMLTGLIPASEGKAWLF 179 IE + +T RFG+ AV VD IR+GEIFG +G +G GKST ++ L L + G+ L Sbjct: 2 IEIQAVTQRFGNVEAVRKVDLSIRKGEIFGIIGRSGAGKSTLVRTLNLLNRPTSGRIVL- 60 Query: 180 GRKVDGSDITT---------RQRVGYMSQAFSLYGELTVRQNLVLHARLFQVPESAIAAR 230 DG D+T+ R+ +G + Q F+L +V N+ L L ++ IAA+ Sbjct: 61 ----DGQDLTSLSAAQLREARRGIGMIFQHFNLLSSRSVYDNIALPLELAGKSKAEIAAK 116 Query: 231 VDEMAQRFDLRQVLDKLPEGLPLGVRQRLSLAVAMVHRPELLILDE 276 V+ + + L + D+ P + G +QR+ +A A+ + P++L+ DE Sbjct: 117 VEPLLELVGLTALRDRYPAQISGGQKQRVGIARALANDPKVLLSDE 162 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,368 Number of extensions: 38445 Number of successful extensions: 473 Number of sequences better than 1.0e-08: 117 Number of HSP's gapped: 356 Number of HSP's successfully gapped: 124 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)