BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0214.p (157 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313176|ref|YP_003777268.1| universal stress protein [Herba... 56 2e-09 >gi|300313176|ref|YP_003777268.1| universal stress protein [Herbaspirillum seropedicae SmR1] Length = 145 Score = 55.8 bits (133), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 47/152 (30%), Positives = 74/152 (48%), Gaps = 13/152 (8%) Query: 1 MYNTILVPIDGSVTAESAVGEARALAVLCKARVRLLHVLDMTTLSNGFE-----DPNVYI 55 M+ TILVP DGS ++ A+ A A AR+ + V + S E DP +Y Sbjct: 1 MFKTILVPTDGSERSDKAIATAVDYAKNSGARLIGITVAEPYPFSPLAESSMAIDPALYE 60 Query: 56 ATTRPLALKEAEDML-ARTAALLEKSGITVDIEIMEAMGARVADVIIERAVTNKADLIVI 114 + LA + + + A TAA G+ EI+ M + II+ A + D I + Sbjct: 61 ENVKTLAQQHIKKITDAATAA-----GVPCQTEIV--MSFNPDEEIIKAAQLHACDAIFM 113 Query: 115 GTHGRRGVHRFLMGSDAEQVARLSPVPVLLVK 146 +HGR G+ R +GS ++V S +PVL+++ Sbjct: 114 ASHGRSGLSRIFLGSKTQKVLTHSTIPVLVLR 145 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 431,070 Number of extensions: 15784 Number of successful extensions: 66 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 66 Number of HSP's successfully gapped: 1 Length of query: 157 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 79 Effective length of database: 1,250,540 Effective search space: 98792660 Effective search space used: 98792660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)