BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0206.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 699 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 535 e-153 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 325 6e-90 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 253 2e-68 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 117 2e-27 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 699 bits (1803), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/439 (75%), Positives = 395/439 (89%) Query: 20 MDVTVIATSLPALARDLGQDPITLKLALTSYVVSLGVFIPISGWIADKVGARTIFRSAMI 79 MD TV+ATSLPALARDL QDPITLKLA+T+YV +LGVFIPISGW+ADK+GAR +F +AMI Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 80 TFMTGSILCALSDSLLTFVAARFLQGIGGAMMVPVGRIIIVRSVSKAELVKAISYLTLPS 139 FMTGSILCA+S SL+TFVAARFLQGIGGAMMVPVGR+II RSV K+ELVKA+SYLTLP+ Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 140 LMGPVIGPPLGGFITTYFHWRWIFLINIPICILGLYLAGRYIQNFREEDPAPLDVKGFLL 199 L+GPV+GP LGGFITTYFHWRW+FLINIP C LGLYLA R+IQNF+E++PAPLDVKGF+L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 200 TACGGGVAMLGFSLIGNHLVPQAWPIVMCVAGSVALFFYFRHAMRSEYPLLDLRLLKIPT 259 T+ G + MLG SL G+HLV A P+ MC G++AL Y+RH+ + E+PLLD RLL+IPT Sbjct: 181 TSVGAAMIMLGLSLFGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLRIPT 240 Query: 260 LRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCASAFGAMFMKALAAMVLR 319 L ASV+GGSLFR+GLGAVPFLLPLALQEGLG+ F+AG+ITCASAFG++F++ LA VLR Sbjct: 241 LHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLR 300 Query: 320 RYGFRTVLMVNAVFAGMAIASYGIFSSHTPYYVMLLVVLMGGFFPSLQFTCLNSIAYADI 379 RYGFRTVLMVNAVFAG+A+A+YGIFS HTPY +++ +VLMGG FP+LQFTCLNSIAYADI Sbjct: 301 RYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADI 360 Query: 380 SNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHATIVEADFWPAFLVVGLFSV 439 +RDAGR TSLASV+QQLSLG+GVT+AG+VLQ+SN+LQGHATIVEADFWPAFLV+GLFSV Sbjct: 361 DSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVIGLFSV 420 Query: 440 ASVPVTARLPKNAGMELTR 458 ASVP++ +LP NAG+EL+R Sbjct: 421 ASVPISGKLPHNAGLELSR 439 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 535 bits (1378), Expect = e-153, Method: Compositional matrix adjust. Identities = 269/437 (61%), Positives = 332/437 (75%) Query: 20 MDVTVIATSLPALARDLGQDPITLKLALTSYVVSLGVFIPISGWIADKVGARTIFRSAMI 79 MD TVI+TSLPALA D+ DPI+LKLALTSY+VSL VFIPISGW+AD+ G+ TIFRSA+ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 80 TFMTGSILCALSDSLLTFVAARFLQGIGGAMMVPVGRIIIVRSVSKAELVKAISYLTLPS 139 FM GSILC +S +L FVAARFLQGIGGAMMVPVGR++I+RSV K LVKA+SYLT+P+ Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 140 LMGPVIGPPLGGFITTYFHWRWIFLINIPICILGLYLAGRYIQNFREEDPAPLDVKGFLL 199 L GPV+GPPLGGFITTYFHWRWIF INIPI ILGLYLA R+I + + E APLD+KGFLL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 200 TACGGGVAMLGFSLIGNHLVPQAWPIVMCVAGSVALFFYFRHAMRSEYPLLDLRLLKIPT 259 +A G + M+G + G HLV + +AG V L Y HA R+ PL+DL LLKIPT Sbjct: 181 SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPT 240 Query: 260 LRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCASAFGAMFMKALAAMVLR 319 LRA V GGS +RVG+GA+PFLLPL LQ G G PF +G +TCASA GAMFMK +AA LR Sbjct: 241 LRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLR 300 Query: 320 RYGFRTVLMVNAVFAGMAIASYGIFSSHTPYYVMLLVVLMGGFFPSLQFTCLNSIAYADI 379 RYGFR VL+VN + + + F+ TPY +M++++L+GG F SLQFT LN+I+YAD+ Sbjct: 301 RYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNAISYADV 360 Query: 380 SNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHATIVEADFWPAFLVVGLFSV 439 +ATS+ASV QQLS+GMGV +AG LQ+SN +QGHAT+V DFWPAFLVVG + Sbjct: 361 DKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQDFWPAFLVVGAVAA 420 Query: 440 ASVPVTARLPKNAGMEL 456 ++ + +LP +AG E+ Sbjct: 421 CALLMLRQLPHDAGAEM 437 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 325 bits (832), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 194/462 (41%), Positives = 278/462 (60%), Gaps = 16/462 (3%) Query: 6 IVPLIVGCALFMENMDVTVIATSLPALARDLGQDPITLKLALTSYVVSLGVFIPISGWIA 65 ++P IV FME +D T+IAT+LP +A+ G P L L +T+Y+++L VFIPISGW+A Sbjct: 31 MLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVA 90 Query: 66 DKVGARTIFRSAMITFMTGSILCALSDSLLTFVAARFLQGIGGAMMVPVGRIIIVRSVSK 125 D+ G+RT+F A+ F SILC S ++ F AAR LQG+GGAMMVPVGR+I+VRS K Sbjct: 91 DRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGK 150 Query: 126 AELVKAISYLTLPSLMGPVIGPPLGGFITTYFHWRWIFLINIPICILGLYLAGRYIQNFR 185 L+KAI+ +T P+++ PV+GP +GGFITTY W WIFL+N+P + ++N Sbjct: 151 DRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEH 210 Query: 186 EEDPAPLDVKGFLLTACGGGVAMLGFSLIGNHLVPQAWPIVMCVAGSVAL-----FFYFR 240 PLD+ GFLL+ A L L G + + G VAL + +R Sbjct: 211 GVSKRPLDLVGFLLSG-----AALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWR 265 Query: 241 HAMRSEYPLLDLRLLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTIT 300 H R E+PLLD L+IPT +VL GSL R+G+ AVP+L+PL Q G GL F++G + Sbjct: 266 HMQRIEHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLL 325 Query: 301 CASAFGAMFMKALAAMVLRRYGFRTVLMVNAVFAGMAIASYGIFSSHTPYYVMLLVVLMG 360 A+A G + MKA +L+R+GFR+V + NA AG I + G+ + TP V+L V+ Sbjct: 326 LATALGNLGMKAFTTRILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCY 385 Query: 361 GFFPSLQFTCLNSIAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHA 420 G S+QF+ L ++AYADIS+ A++L SV QQ+++GMG+ L L+I+ L+G A Sbjct: 386 GLSRSMQFSTLATLAYADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEA 445 Query: 421 TIVE------ADFWPAFLVVGLFSVASVPVTARLPKNAGMEL 456 DF AF + G+ ++ SV L ++AG + Sbjct: 446 IDASGAHFTLGDFQWAFAMAGVVTLISVIGYRGLARDAGSNI 487 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 253 bits (646), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 153/438 (34%), Positives = 247/438 (56%), Gaps = 15/438 (3%) Query: 10 IVGCALFMENMDVTVIATSLPALARDLGQDPITLKLALTSYVVSLGVFIPISGWIADKVG 69 IV FM+ +D T++ T+LPA+AR LG+ + + + +Y +++ P SGW+AD+ G Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 70 ARTIFRSAMITFMTGSILCALSDSLLTFVAARFLQGIGGAMMVPVGRIIIVRSVSKAELV 129 R ++ +A++ F GS+LCA++ SL +AAR +QG+GG+M++PVGR+ ++R+V A+ + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 130 KAISYLTLPSLMGPVIGPPLGGFITTYFHWRWIFLINIPICILGLYLAGRYIQNFREEDP 189 A++ +++ +GPV+GP LGG++ W WIFLIN+P+ +LG+ ++ + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGT 236 Query: 190 APLDVKG-FLLTACGGGVAMLGFSLIGNHLVP-----QAWPIVMCVAGSVALFFYFRHAM 243 D+ G LL+AC M+ FSL + VP W I + V ++ + Y+RHA Sbjct: 237 PRFDLLGCVLLSAC-----MVAFSLALD--VPFDSHRTGWSIALFVVAALTVLGYWRHAS 289 Query: 244 RSEYPLLDLRLLKIPTLRASVLGGSLFRVGLGAVPFLLPLALQEGLGLNPFEAGTITCAS 303 PL L L P + G + R+G AVPFLLPL LQ LG +P AG + + Sbjct: 290 VHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPA 349 Query: 304 AFGAMFMKALAAMVLRRYGFRTVLMVNAVFAGMAIASYGIFSSHTPYYVMLLVVLMGGFF 363 A K A +++R+G+ L+VN G AI S+ + + P ++ + + G Sbjct: 350 AIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGAS 409 Query: 364 PSLQFTCLNSIAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQISNYLQGHATIV 423 S+QF +NS+ + RDAG SL S+VQ L++G+GVTI G ++ + G AT Sbjct: 410 NSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTAT-- 467 Query: 424 EADFWPAFLVVGLFSVAS 441 A F F +VG ++AS Sbjct: 468 NASFQITFAIVGAITLAS 485 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 118/431 (27%), Positives = 206/431 (47%), Gaps = 29/431 (6%) Query: 9 LIVGCALFMENMDVTVIATSLPALARDLGQDPITLKLALTSYVVSLGVFIPISGWIADKV 68 L V A+FM +D ++ ++P ++ +LG +TS+ + + IP++GW+ ++ Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 69 GARTIFRSAMITFMTGSILCALSDSLLTFVAARFLQGIGGAMMVPVGRIIIVRSVSKAEL 128 GA +F ++++ F+ S LC L+ +L +AAR LQG+ MVP+ + +++ + K++ Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKS 146 Query: 129 VKAISYLTLPSLMGPVIGPPLGGFITTYFHWRWIFLINIPICILGLYLAGRYIQNFR-EE 187 A++ + +++ PV GP LGG+IT + W WIF INIP+ GL AG + +R E Sbjct: 147 SFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPV---GLIAAGVTLAIYRSRE 203 Query: 188 DPA---PLDVKG--FLLTACGGGVAMLGFSLIGNHLVPQAWPIVMCVAGSVAL--FFYF- 239 P P+D G L+T ML G L A P ++ + G +AL F YF Sbjct: 204 TPTHKLPIDKIGLALLVTWVAAFQIMLD---KGKDLDWFASPTIVTL-GIIALIAFAYFV 259 Query: 240 RHAMRSEYPLLDLRLLKIPTLRASVLGGSL-FRVGLGAV---PFLLPLALQEGLGLNPFE 295 + +++P++DLRL R + GG++ +G G +LP LQ+ LG + Sbjct: 260 VWELNNDHPVVDLRLFG----RRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSID 315 Query: 296 AGTITCASAFGAMFMKALAAMVLRRYGFRTVLMVNAVFAGMAIASY--GIFSSHTPYYVM 353 +G T A+ + L L R V A F G AI + ++ + Sbjct: 316 SGLSTAPIGIFAVILMPLIGRFLPLVDARKV--ATASFIGFAIVFWLRSRYNLQVDQMTV 373 Query: 354 LLVVLMGGFFPSLQFTCLNSIAYADISNRDAGRATSLASVVQQLSLGMGVTIAGLVLQIS 413 +L L+ G ++ F L + + + A L+S V+ +G +I+ + Sbjct: 374 ILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN-N 432 Query: 414 NYLQGHATIVE 424 + HA + E Sbjct: 433 RSIMHHAQLTE 443 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,396,408 Number of extensions: 59137 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 313 Number of HSP's successfully gapped: 17 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)