BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0203.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Her... 793 0.0 gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Her... 622 e-179 gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspir... 82 1e-16 >gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 575 Score = 793 bits (2049), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/573 (71%), Positives = 453/573 (79%), Gaps = 25/573 (4%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+ KY VDLPDR+WPSRT+ +AP WLSTDLRDGNQALFEPMN ERKL LFHE Sbjct: 1 MLTDPSQKYRAIAPVDLPDRRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 LVR+GFKEIEVGFPA S+ DF+ VR LI + IP DVTPMVMTQLR + I TV+S GA Sbjct: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 R IVHFYNA+AP WRE+VFG+SVPQ++ L+E ++ L + LTA HPETEWILQYSPETFC Sbjct: 121 RRVIVHFYNAIAPAWREIVFGMSVPQIITLVEDHIALFRRLTAVHPETEWILQYSPETFC 180 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 MAELEVSL+ C AI WDAGP R +IINLPTTVEVSTPNVFADQIEWM RRL RREH++ Sbjct: 181 MAELEVSLEVCNAAIRAWDAGPRRRMIINLPTTVEVSTPNVFADQIEWMDRRLERREHLI 240 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 LSVHPHNDRGT VACAEQA+LAGAQRVEGCLFGNGERSGN+DVVTLALNLYT GI P LD Sbjct: 241 LSVHPHNDRGTAVACAEQAMLAGAQRVEGCLFGNGERSGNVDVVTLALNLYTQGIAPGLD 300 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDIAA+ARVAE CTALPIHPRHPYVGDLVFTAFSGSHQDAI KGFAAQ+PD WRVPYL Sbjct: 301 FSDIAALARVAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQRPDAPWRVPYL 360 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDP D+GRTYDSIVRVNSQSGKGGIAFLL+RDH I MPRRMQVEFSAIVQ ADASETE Sbjct: 361 PIDPTDLGRTYDSIVRVNSQSGKGGIAFLLQRDHHITMPRRMQVEFSAIVQALADASETE 420 Query: 420 ISSAALWELFERTYL----ASPR-----------QDGRVRYLSHQLSNPGAAQDIALELT 464 ++S LW++FERTYL ASP + R Y SH L + I LEL Sbjct: 421 LTSEHLWDVFERTYLAPVQASPDFSDRTSLAPALEQRRFIYRSHCLHPHPDGERIVLELA 480 Query: 465 VSGVAIR-LNGNGNGLIAATVDALRSRLTGLEGLRIDSYEERS--IGSGAQAQAWAIVEA 521 + +R + G+GNG IAATV AL GL LRIDSYEERS +G GA AQA AIVEA Sbjct: 481 DTEGNVRTVEGSGNGPIAATVAAL-----GL-ALRIDSYEERSLGVGVGADAQALAIVEA 534 Query: 522 ASPGVAGTRFGAARHVNIVTASILAVLSAANRL 554 A P V G+RFG RH NI TASI+AVLSAA+R Sbjct: 535 ALPTVPGSRFGVGRHENITTASIMAVLSAASRF 567 >gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 567 Score = 622 bits (1605), Expect = e-179, Method: Compositional matrix adjust. Identities = 307/557 (55%), Positives = 403/557 (72%), Gaps = 14/557 (2%) Query: 1 MIAHSRTKYPHPPTVDLPDRQWPSRTIGKAPIWLSTDLRDGNQALFEPMNAERKLTLFHE 60 M+++ KY P +DLPDR WPS+ I PIW+STDLRDGNQAL EPMNAERKL F Sbjct: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVINTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 Query: 61 LVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPADVTPMVMTQLRGEQIVRTVES-HGA 119 L++ G KEIEVGFP+ SQTDFD VR LI + IP DVT +V+TQ R E I RT+ES GA Sbjct: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTIESLEGA 121 Query: 120 PRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVRLLKDLTAQHPETEWILQYSPETFC 179 +AIVH YN+VAP +R++VF +S ++ + RL+K+LT P TEW +YSPE+F Sbjct: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 Query: 180 MAELEVSLQACRVAIDTWDAGPDRPIIINLPTTVEVSTPNVFADQIEWMHRRLPRREHVV 239 EL+ S + C +TW A P+R +I+NLP+TVE +TPNV+ADQIEWM R L R + Sbjct: 182 TTELDFSKEICDAVCETWGASPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 Query: 240 LSVHPHNDRGTGVACAEQALLAGAQRVEGCLFGNGERSGNLDVVTLALNLYTNGIDPQLD 299 +SVHPHNDRGT VA AE A++AGA RVEGCLFGNGER+GN+D+VTLALNLYT G++P LD Sbjct: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 Query: 300 FSDIAAVARVAENCTALPIHPRHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVPYL 359 FSDI V +V E C +P+HPRHPYVGDLVFTAFSGSHQDAI+KGFA Q+PD +W VPYL Sbjct: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 Query: 360 PIDPQDIGRTYDSIVRVNSQSGKGGIAFLLERDHGIVMPRRMQVEFSAIVQKHADASETE 419 PIDP D+GR+YD+++RVNSQSGKGG+A+LLE+++G+ +PRR+Q+EFS +Q+ ADA+ E Sbjct: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 Query: 420 ISSAALWELFERTYLASPRQDGRVRYLSHQLS-NPGAAQDIALELTV--SGVAIRLNGNG 476 I+++ + +F+R YL R + V Y +H++S + A+ I +E+ + +G + + G+G Sbjct: 422 IAASDIHAIFQREYLE--RVEPYV-YRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSG 478 Query: 477 NGLIAATVDALRSRLTGLEGLRIDSYEERSIGSGAQAQAWAIVEAASPGVAGTRFGAARH 536 NG I A V AL GL+ +++ + E +IG+GA A+A + +E A T FG Sbjct: 479 NGPIDAFVHAL-----GLD-IKLMDFHEHAIGAGADAKAASYIE-LRLNEAPTGFGVGID 531 Query: 537 VNIVTASILAVLSAANR 553 NIVTAS AVLSA NR Sbjct: 532 ANIVTASFKAVLSAVNR 548 >gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 82.0 bits (201), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 89/367 (24%), Positives = 150/367 (40%), Gaps = 68/367 (18%) Query: 36 TDLRDGNQALFEPMNAERKLTLFHELVRLGFKEIEVGFPAGSQTDFDVVRTLITDKHIPA 95 T LRDG Q AE K+ + L +G E+E+G P Q + +V++ + +P Sbjct: 7 TTLRDGEQTAGVAFTAEEKIDIARALDEIGVPEMEIGIPIMGQAEIEVIQAIAA---LPL 63 Query: 96 DVTPMVMTQLRGEQIVRTVESHGAPRAIVHFYNAVAPLWREVVFGLSVPQVMQLIERNVR 155 MV ++ + A A + LS+P Sbjct: 64 RSKTMVWGRMCEADL------------------AAAARCNADIVNLSMP----------- 94 Query: 156 LLKDLTAQHP---ETEWILQYSPETFCMAELEVSLQACRVAIDTWDAGPDRPIIINLPTT 212 + D+ QH + +W+L F L++ ++ C D+ A D ++ + T Sbjct: 95 -VSDIHIQHKLQRDRDWVLA-QIRHFLPKALDLGMEVCLGGEDSSRA--DLDFMLRVIET 150 Query: 213 VEVSTPNVF--ADQIEWMH--------RRLPRREHVVLSVHPHNDRGTGVACAEQALLAG 262 + + F AD + + R+L + + L +H HND G A A+ AG Sbjct: 151 AQAAGARRFRFADTLGLLDPFATYEVIRQLRQEVDIELEMHAHNDLGLATANTLAAINAG 210 Query: 263 AQRVEGCLFGNGERSGN--LDVVTLALNLYTNGIDPQLDFSDIAAVARVAENCTALPIHP 320 A V + G GER+GN L+ V + L + +GI+ Q+D + A++R+ + + P Sbjct: 211 ASHVNTTVNGLGERAGNAPLEEVVMGLR-HLHGIETQVDTRSLPAISRLVAKASGKAVAP 269 Query: 321 RHPYVGDLVFTAFSGSHQDAIRKGFAAQKPDGLWRVP--YLPIDPQDIGRTYDSIVRVNS 378 VG+ VFT SG H DGL + P Y P ++GR++ + + Sbjct: 270 NKSIVGEAVFTHESGIH------------VDGLLKNPANYEHFSPAELGRSHHIV--LGK 315 Query: 379 QSGKGGI 385 SG G+ Sbjct: 316 HSGSRGV 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,788,102 Number of extensions: 76852 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 223 Number of HSP's successfully gapped: 3 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)