BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0189.p (194 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312650|ref|YP_003776742.1| carbonic anhydrase [Herbaspiril... 74 9e-15 >gi|300312650|ref|YP_003776742.1| carbonic anhydrase [Herbaspirillum seropedicae SmR1] Length = 212 Score = 73.9 bits (180), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 53/170 (31%), Positives = 82/170 (48%), Gaps = 9/170 (5%) Query: 3 LGSQRSPEYFWIGCSDNRVPANDLLGLLPGELFVHRNIANVVVHTDLNCLSV---LQFAV 59 L + ++P+ +I CSD+R+ + PG+LFV RN N+V V +++AV Sbjct: 26 LATGQTPKALFISCSDSRMVPELVTQREPGDLFVIRNAGNIVPSFGPEPGGVSATVEYAV 85 Query: 60 DVLKVKHIIICGHYGCAGVQAALT---KRRVGLADNWLQHVQDVHEKHIGCFHDHMSQGD 116 LKV I+ICGH C + A T + NWL H + I H ++ D Sbjct: 86 AQLKVSDIVICGHSDCGAMTAVATCACLDHMPAVRNWLHHTDAA--RMINESRPHATEKD 143 Query: 117 QHDRLCELNVLEQVANICRTTIVTDAWERGQELSIHALVYGVHDGLLEAL 166 + D + NV+ Q+ NI V A +G+ L++H VY + G + AL Sbjct: 144 RIDGMVRENVIAQLNNIRTHPSVAVALAQGR-LALHGWVYDIESGSILAL 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 597,843 Number of extensions: 21792 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 194 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 114 Effective length of database: 1,241,070 Effective search space: 141481980 Effective search space used: 141481980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)