BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0181.p (122 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 157 3e-40 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 55 1e-09 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 54 3e-09 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 54 4e-09 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 157 bits (396), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 78/103 (75%), Positives = 88/103 (85%) Query: 1 MKKDAARDALVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSI 60 M KD+AR A+VQEARELLVAMEAALLQ E +G SRD+I+AIFRAAHTIKGSAGLFAFDSI Sbjct: 1 MSKDSARHAMVQEARELLVAMEAALLQTEMEGPSRDSIHAIFRAAHTIKGSAGLFAFDSI 60 Query: 61 VSFTHLLENVLEKVRNGIVKLDDGMMSLLLNCGDYMGELVDAI 103 V FTH LE+VLE+VR + LD +MSLLL C DY+GELVDAI Sbjct: 61 VQFTHHLEHVLERVREERLALDSHLMSLLLQCSDYIGELVDAI 103 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 55.5 bits (132), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 27/82 (32%), Positives = 44/82 (53%) Query: 21 MEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHLLENVLEKVRNGIVK 80 ME LL + + +NA+FRAAH+IKG + F I TH+LE++L+++R G + Sbjct: 1 MEKLLLAVNVASPDPEDLNAVFRAAHSIKGGSSTFGLTDIAEVTHVLESLLDRIRQGQMA 60 Query: 81 LDDGMMSLLLNCGDYMGELVDA 102 L + L D + ++D Sbjct: 61 LTSEHVDAFLAAKDVLKSMLDG 82 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 54.3 bits (129), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 29/68 (42%), Positives = 39/68 (57%) Query: 10 LVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHLLEN 69 V EA EL +E LL +E + +NA+FRA HTIKG AG ++VS HL EN Sbjct: 9 FVVEALELATNVEEHLLTLERHPDDLEILNAVFRAFHTIKGGAGFVNLPAMVSACHLTEN 68 Query: 70 VLEKVRNG 77 + + +R G Sbjct: 69 LFDALRTG 76 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 53.9 bits (128), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 28/97 (28%), Positives = 51/97 (52%) Query: 7 RDALVQEARELLVAMEAALLQIESDGASRDAINAIFRAAHTIKGSAGLFAFDSIVSFTHL 66 RD + E R+LL + L+Q+E + + ++FR HT+KG++GLF + H Sbjct: 5 RDQFILETRDLLQGISEKLMQLEDAPEHVELLESLFRMVHTLKGNSGLFDVPEMTRVLHA 64 Query: 67 LENVLEKVRNGIVKLDDGMMSLLLNCGDYMGELVDAI 103 E++++ VR V + + LL+ D++ L D + Sbjct: 65 CEDLMDAVRRRQVAYSEVLADRLLDAMDFVAMLCDGL 101 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 342,589 Number of extensions: 11784 Number of successful extensions: 49 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 49 Number of HSP's successfully gapped: 4 Length of query: 122 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 48 Effective length of database: 1,269,480 Effective search space: 60935040 Effective search space used: 60935040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)