BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0172.p (533 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis tra... 295 9e-81 gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis tra... 208 8e-55 gi|300311931|ref|YP_003776023.1| methyl-accepting chemotaxis II ... 190 2e-49 gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis tra... 185 7e-48 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 166 6e-42 >gi|300309958|ref|YP_003774050.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 295 bits (754), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 178/339 (52%), Positives = 245/339 (72%), Gaps = 13/339 (3%) Query: 159 LVDE----NVHQAKANKSRAAILERKTLGEVVAVTALALLIAVALTLYMRRIFARQIGGD 214 LVDE NV QA + + LE+K ++A + + L + L+ RI QIGG+ Sbjct: 213 LVDERTQANVRQAGKDYAE---LEQKIFW-LLAASVVVLFACLWLSY---RIIRSQIGGE 265 Query: 215 PREVMRILRKIADGDLRVQFAVGKHGQGSVLHSAQRMLSQLSQVLRDVNTAAATLASASG 274 PR+ M +LR++A+GDL V + K + SVL+S Q+M+++ + V+ DV+ A +LASAS Sbjct: 266 PRDAMSVLRRVAEGDLTVVVPLPKGDKLSVLYSTQQMINKWTDVIGDVSGTATSLASASE 325 Query: 275 QLTAAAEQLSKNSSQQAVDSEETSCSIGQISATVSQNTANANHTNVIANESARAATEGRE 334 Q++++++ LS N++QQA + EETS S+ +I+AT++QN NA T+ IA+ SA AATEG E Sbjct: 326 QISSSSQALSHNAAQQAANVEETSASVEEITATIAQNADNARTTDGIASLSASAATEGGE 385 Query: 335 AVRETLEAMHQIVAKIGVIDDIAYQTNLLALNAAXPP--RGENGKGFAVVANEVRKLAER 392 AVRET+ AM QI +KIG+IDDIAYQTNLLALNAA GE+GKGFAVVA EVRKLAER Sbjct: 386 AVRETVAAMKQIASKIGIIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAER 445 Query: 393 SQIAAQEIVSVSDHSVGLAEKAGTLLEGMLPSIQQTADLVDAISLSARDQNASLVQIHSA 452 SQ AAQEI++V++ SVGLAEKAG LL M+PSI++TADLV I+ ++ +Q+ L QI++A Sbjct: 446 SQSAAQEIIAVAETSVGLAEKAGGLLNQMVPSIRKTADLVQEIAAASHEQSTGLEQINNA 505 Query: 453 IGQITQAIQLNAVASEELSATSEEMNEQAHQLRELMGYF 491 + Q+ Q Q+ A ASEELSATSEEM+ QA L++LM +F Sbjct: 506 VHQMAQTTQMTASASEELSATSEEMSAQAIHLQDLMRFF 544 >gi|300310428|ref|YP_003774520.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 208 bits (530), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 136/325 (41%), Positives = 207/325 (63%), Gaps = 8/325 (2%) Query: 174 AAILERKTLGEVVAVTALA----LLIAVALTLYMRRIFARQIGGDPREVMRILRKIADGD 229 +A+ R+ +G+ + AL LLI + + R RQ+GG+P +V+ ++++++DGD Sbjct: 181 SALTAREIIGQALETVALVAGGVLLILLGAGWLLTRTLLRQMGGEPGDVVTVMQRLSDGD 240 Query: 230 LRVQFAVGKHG-QGSVLHSAQRMLSQLSQVLRDVNTAAATLASASGQLTAAAEQLSKNSS 288 L VQ K G + S++H+ Q+M+++L+QV+ +V A L S + +++A A+ LS+ S+ Sbjct: 241 L-VQTITLKPGCRDSMMHAIQKMMTRLAQVVGEVRGNADGLVSTAEEVSATAQSLSRAST 299 Query: 289 QQAVDSEETSCSIGQISATVSQNTANANHTNVIANESARAATEGREAVRETLEAMHQIVA 348 +QA E+TS ++ QI A + NA T+ IA +A A + E V++ + A+ QI Sbjct: 300 EQANGVEQTSEALAQIHALLQSTAQNARLTDQIAAAAALEARQCAETVKQMVAAIQQIAG 359 Query: 349 KIGVIDDIAYQTNLLALNAAXPP--RGENGKGFAVVANEVRKLAERSQIAAQEIVSVSDH 406 KI VIDDIAYQTNLLALNA GE+G GFAVVA EVR+LAERSQ AAQEI V+ Sbjct: 360 KIAVIDDIAYQTNLLALNATIEAAHAGEHGSGFAVVAAEVRRLAERSQSAAQEIDEVARA 419 Query: 407 SVGLAEKAGTLLEGMLPSIQQTADLVDAISLSARDQNASLVQIHSAIGQITQAIQLNAVA 466 +V LA A L M+P+I +T+ LV I S+ Q+ ++ QI +A Q+ Q IQ N+++ Sbjct: 420 NVTLACAAERQLSDMVPTITRTSVLVQDIVQSSEQQSTAVSQISTAASQLAQVIQQNSLS 479 Query: 467 SEELSATSEEMNEQAHQLRELMGYF 491 SEEL+ TSEE+N +A L+ +G+F Sbjct: 480 SEELAVTSEELNRRALLLQTAVGFF 504 >gi|300311931|ref|YP_003776023.1| methyl-accepting chemotaxis II protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 190 bits (483), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 123/300 (41%), Positives = 185/300 (61%), Gaps = 3/300 (1%) Query: 209 RQIGGDPREVMRILRKIADGDLRVQFAVGKHGQGSVLHSAQRMLSQLSQVLRDVNTAAAT 268 R++GG+P I+R+IADG+L V+ + + + S+L++ M ++L++++R+V + Sbjct: 240 RELGGEPAYAADIVRQIADGNLCVEVNLRSNDRSSLLYAMHTMRARLTEIMRNVTLSTQA 299 Query: 269 LASASGQLTAAAEQLSKNSSQQAVDSEETSCSIGQISATVSQNTANANHTNVIANESARA 328 L +S QL A A L++ SS+QA EE +I ++ + Q +A TN IA +A Sbjct: 300 LGVSSRQLNATAHALAQASSEQAAAVEEVHGAIASMNQAIQQTGEHARRTNQIAVAAASD 359 Query: 329 ATEGREAVRETLEAMHQIVAKIGVIDDIAYQTNLLALNAAXPP--RGENGKGFAVVANEV 386 A + AV+ T++AM I ++GVIDDIAYQTNLLALNAA GE+G+GF+VVA EV Sbjct: 360 ADKSGAAVQTTVDAMRGIARQVGVIDDIAYQTNLLALNAAIEAARAGEHGRGFSVVAAEV 419 Query: 387 RKLAERSQIAAQEIVSVSDHSVGLAEKAGT-LLEGMLPSIQQTADLVDAISLSARDQNAS 445 RKLAERSQ +A EI ++ SV LAE+ L+EG L I+Q + ++ I+LS+R Q Sbjct: 420 RKLAERSQSSAHEISVIAQQSVELAEQTSQRLVEGTLQGIRQASQQINQITLSSRMQEEG 479 Query: 446 LVQIHSAIGQITQAIQLNAVASEELSATSEEMNEQAHQLRELMGYFSLDVSDHENESETP 505 + +I A+ Q+ Q NA ASEEL+ T+E M E+A +L + YF L+ S + P Sbjct: 480 VAEIGVAVTQLNDTTQRNAAASEELAITAEAMAERARELNAQLAYFRLEQSQQGQPGQRP 539 >gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 185 bits (470), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 163/520 (31%), Positives = 269/520 (51%), Gaps = 33/520 (6%) Query: 1 MKLSSKLAVCFAFGALLTAAVGADGVLNLFRVNAMLQEVYTANLLTIVNLNNANKIAVDI 60 +K+S KL F AL+ A +G G++ ++N + E+Y L +V L ++ + +D+ Sbjct: 7 LKVSHKLIGGFMIVALIGATIGTFGIIKAGQINDLATEMYE---LQVVGLRHSAQATLDL 63 Query: 61 ATSMQNMEKS---EERVERQRIADVLKYRLKDLGQAYDGYKSTTVTSELERTLQQQADVL 117 ++S + + + E ER+ + L RL + A + + VT E + L +A Sbjct: 64 SSSNRAIRTAILAESPAEREASLNDLTKRLDNTTLALNEAEQRFVTPE-GKALVAKAREA 122 Query: 118 LDAYVKRVNQRREQLLRDEDAKGNPDSLRIESDSLRSQLAALVDENVHQ-AKANKSRAAI 176 Y V Q E+L ++ A + R+ S S+R LA + N+ + + K A Sbjct: 123 FTKYKNAVMQTAEELRKEPLADARQSTSRLFS-SVRP-LANEAEANMLKLMEVKKDNADR 180 Query: 177 LERKT-------LGEVVAVTALALLIAVALTLYMRRIFARQIGGDPREVMRILRKIADGD 229 L +T ++ +T +L +AL L++ R RQ+GG+PR+V IA GD Sbjct: 181 LNDETDLIYAHIRTLLLVLTFGGVLFGIALGLFISRNLTRQLGGEPRDVAAAADAIASGD 240 Query: 230 LRVQFAVGKHGQGSVLHSAQRMLSQLSQVLRDVNTAAATLASASGQLTAAAEQLSKNSSQ 289 L + QGSV+ + M L +V++ V T++ ++A+ S Q+ LS + Q Sbjct: 241 LSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLDLSARTEQ 300 Query: 290 QAVDSEETSCSIGQISATVSQNTANANHTNVIANESARAATEGREAVRETLEAMHQIVAK 349 QA EET+ S+ ++++TV N +NA + +A ++ A EG E V + ++ M I Sbjct: 301 QASALEETASSMEELTSTVKHNASNAQNAKEMAATASTVAQEGGEVVAQVVDTMSAINES 360 Query: 350 -------IGVIDDIAYQTNLLALNAAXPP--RGENGKGFAVVANEVRKLAERSQIAAQEI 400 I VID IA+QTN+LALNAA GE G+GFAVVA EVR LA+RS AA+EI Sbjct: 361 SRKMADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRNLAQRSAAAAKEI 420 Query: 401 VSVSDHSVG-------LAEKAGTLLEGMLPSIQQTADLVDAISLSARDQNASLVQIHSAI 453 ++ + SV LA++AGT + G++ + + ++ IS ++ +Q A + QI+ AI Sbjct: 421 KALIEDSVDKVTVGARLADQAGTTMSGIVENAGKVTSIIGEISAASTEQTAGIEQINQAI 480 Query: 454 GQITQAIQLNAVASEELSATSEEMNEQAHQLRELMGYFSL 493 Q+ Q NA EE +A S + +QA L +++ F+L Sbjct: 481 MQMDDVTQQNASLVEEAAAASRALQDQADALAQIVSIFNL 520 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 166 bits (419), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 110/332 (33%), Positives = 191/332 (57%), Gaps = 16/332 (4%) Query: 179 RKTLGEVVAVTALALLIAVALTLYMRRIFARQIGGDPREVMRILRKIADGDLRVQFAVGK 238 R L + A ++L+AVA + R Q+GG+P + + +IA G+L V + K Sbjct: 187 RSILTTTWTLVAGSVLLAVAAAFLIVRSVLHQLGGEPAQAQELAAQIAQGNLAVSIPLAK 246 Query: 239 HGQGSVLHSAQRMLSQLSQVLRDVNTAAATLASASGQLTAAAEQLSKNSSQQAVDSEETS 298 Q S++ S + M +QL++++ + +A ++++A+ Q+ LS+ + +QA EET+ Sbjct: 247 GDQHSLMASLENMRAQLTRIVDGIKHSAESISTAASQIAVGNLDLSRRTEEQAASLEETA 306 Query: 299 CSIGQISATVSQNTANANHTNVIANESARAATEGREAVRETLEAM-------HQIVAKIG 351 S+ +I++TV QNT NA + +A ++ A +G E V + M ++I I Sbjct: 307 SSMEEITSTVEQNTQNAGQGHQLARSASLHAQKGGEVVERVVGTMKDISQSAYKITEIIA 366 Query: 352 VIDDIAYQTNLLALNAAXPP--RGENGKGFAVVANEVRKLAERSQIAAQEIVSVSDHSV- 408 ++ IA+QTN+LALNAA GE G+GFAVVA EVR LA+RS +A+EI + + SV Sbjct: 367 TVEGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASSAKEIRGLIESSVQ 426 Query: 409 ------GLAEKAGTLLEGMLPSIQQTADLVDAISLSARDQNASLVQIHSAIGQITQAIQL 462 L ++AG ++ ++ SIQQ DL+ I+ ++ +Q++ + QI+ AI Q+ + Q Sbjct: 427 HVEAGQKLVDEAGLTMDEVVRSIQQVNDLMSEITAASNEQSSGISQINIAISQMDEVTQQ 486 Query: 463 NAVASEELSATSEEMNEQAHQLRELMGYFSLD 494 NA EE ++ ++ M +QA+ LR+ + F ++ Sbjct: 487 NAALVEESASAAQSMAQQANSLRDAVAVFRIE 518 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.324 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,296,336 Number of extensions: 47473 Number of successful extensions: 591 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 281 Number of HSP's successfully gapped: 70 Length of query: 533 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 443 Effective length of database: 1,193,720 Effective search space: 528817960 Effective search space used: 528817960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)