BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0170.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 348 4e-97 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 169 3e-43 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 124 7e-30 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 69 6e-13 gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 61 8e-11 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 348 bits (892), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 174/270 (64%), Positives = 206/270 (76%), Gaps = 2/270 (0%) Query: 9 SMALD--VSDQEMLLFQQLFREHIGLYLPFSKKALLCSRLSRRLGELSLSSLKEYHSMIS 66 +ALD VSD +MLLFQ+LF++ IGL+LP SKKALL SRL+ RL EL L L +YH++IS Sbjct: 6 GIALDEEVSDHDMLLFQRLFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALIS 65 Query: 67 RPQEEAERQRAIDLITTNETYFFREQKHFDALRDQILPQIDRRHPLKVWSAASATGEEAY 126 ERQRAIDLITTNETYFFRE HFD + Q LPQ+ ++ L++WSAASATGEEAY Sbjct: 66 SEAGAQERQRAIDLITTNETYFFREPGHFDFVVGQWLPQLAQQPQLRIWSAASATGEEAY 125 Query: 127 SIAMLLDDQRSQHPWSVLASDISLRVLSFGRRALYPMVRGELIPPPYLSRYCLRGTGEYE 186 ++AMLLD R WSV ASDIS RVL F RRALYPMVRG+ IP YL RYCL G GEYE Sbjct: 126 TLAMLLDHHRPGASWSVFASDISTRVLRFARRALYPMVRGQNIPRAYLRRYCLMGVGEYE 185 Query: 187 GHFLIDQKIRKRVTFEQRNLLSLPAEMDGQFDVVFLRNVIIYFDFPTKVDVLRSVVSKLR 246 GHFL+ +R +V F QRNL L DGQ+D++FLRNVIIYFD TK+ VLR V+++LR Sbjct: 186 GHFLVQADLRAKVQFAQRNLTRLDEHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLR 245 Query: 247 PGGWLVVGHSESLHGMPLELQMHAPSIYRR 276 PGG+LVVGHSESLHGM L L M +PSIYR+ Sbjct: 246 PGGYLVVGHSESLHGMDLGLDMQSPSIYRK 275 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 169 bits (428), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 93/265 (35%), Positives = 150/265 (56%), Gaps = 5/265 (1%) Query: 12 LDVSDQEMLLFQQLFREHIGLYLPFSKKALLCSRLSRRLGELSLSSLKEYHSMISRPQEE 71 D + ++ + L + G+ L SK+ ++ SRL+RRL ++S ++Y M+ R + Sbjct: 16 FDFNSRDFERVRGLIYQRAGIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDS 75 Query: 72 AERQRAIDLITTNETYFFREQKHFDALRDQILPQIDRRHPLKVWSAASATGEEAYSIAML 131 E + + +TTN T FFRE HF L D L + + P +W +A++TGEE Y+IAM Sbjct: 76 EEWEAFTNALTTNLTSFFREAHHFPILAD-FLKTL--KEPATIWCSAASTGEEPYTIAMT 132 Query: 132 LDDQRSQ--HPWSVLASDISLRVLSFGRRALYPMVRGELIPPPYLSRYCLRGTGEYEGHF 189 + P ++A+DI VL+ G +YPM R E + P + ++ LRG G +EG+ Sbjct: 133 ACEAYGSLTPPVQIVATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYV 192 Query: 190 LIDQKIRKRVTFEQRNLLSLPAEMDGQFDVVFLRNVIIYFDFPTKVDVLRSVVSKLRPGG 249 + ++RK +TF+Q NLL+ + GQFDV+F RNV+IYFD PT+ +L V ++P Sbjct: 193 RVRPELRKLITFKQLNLLADGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHA 252 Query: 250 WLVVGHSESLHGMPLELQMHAPSIY 274 L GHSE+ + L++ ++Y Sbjct: 253 LLFAGHSENFLYVSDALKLKGKTVY 277 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 124 bits (312), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 77/254 (30%), Positives = 130/254 (51%), Gaps = 12/254 (4%) Query: 14 VSDQEMLLFQQLFREHIGLYLPFSKKALLCSRLSRRLGELSLSSLKEYHSMISRPQEEAE 73 +S+ + L F++ F G+ +K+ + RL R+ S + Y S++ + E Sbjct: 6 ISEADFLKFREYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKE 65 Query: 74 RQRAIDLITTNETYFFREQKHFDALRDQILPQIDRRH----PLKVWSAASATGEEAYSIA 129 Q +L+T NETYF RE+ F L + +LP+I R P+++W ++GEEAY++A Sbjct: 66 LQLLTNLMTVNETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLA 125 Query: 130 MLLDDQR---SQHPWSVLASDISLRVLSFGRRALYPMVRGELIPPPYLSRYCLRGTGEYE 186 M L ++ Q +++SDI VL + Y + +P ++S+Y LR EY+ Sbjct: 126 MYLMERWPGIDQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQ 185 Query: 187 GHFLIDQKIRKRVTFEQRNLL-SLPAEMDGQFDVVFLRNVIIYFDFPTKVDVLRSVVSKL 245 I +R V F + NL S + FDV+F RN++IYFD ++ + + L Sbjct: 186 ----ICDALRGAVEFTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEAL 241 Query: 246 RPGGWLVVGHSESL 259 G++ +GHSES+ Sbjct: 242 NLNGFICLGHSESM 255 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 60/213 (28%), Positives = 100/213 (46%), Gaps = 22/213 (10%) Query: 78 IDLITTNETYFFREQKHFDALRDQILPQIDRRHPLKVWSAASATGEEAYSIAMLLDDQRS 137 ++ +T + FR+ +F ALR++++P + LK+W A ++GEE YS+A+LL ++ Sbjct: 75 MEYLTVQVSEMFRDPSYFLALREKVVPLLRTYPSLKIWVAGCSSGEEVYSLAILLREEGL 134 Query: 138 QHPWSVLASDISLRVLSFGRRALYPMVRGELIPPPY--------LSRYCLRGTGEYEGHF 189 + A+DI+ L +Y + R + LS Y T Y G Sbjct: 135 LERSIIYATDINQSALKKAEAGVYDLERIAGFTNNHQKSGARTSLSDYY---TAAY-GAV 190 Query: 190 LIDQKIRKRVTFEQRNLL--SLPAEMDGQFDVVFLRNVIIYFDFPTKVDVLRSVVSKLRP 247 + D+ +RK V F +L S+ AEM +V RNV+IYF+ + L L Sbjct: 191 VFDKSLRKNVVFSDHSLATDSVFAEM----HLVSCRNVLIYFNRQLQERALGLFRESLVR 246 Query: 248 GGWLVVGHSESL----HGMPLELQMHAPSIYRR 276 G+L +G ESL H + E + I+++ Sbjct: 247 KGFLGLGSKESLRFSSHAVAFEEVVREDRIFQK 279 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 61.2 bits (147), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 57/192 (29%), Positives = 95/192 (49%), Gaps = 16/192 (8%) Query: 75 QRAIDLITTNETYFFREQKHFDAL----RDQILPQIDRRHPLKVWSAASATGEEAYSIAM 130 Q I+L ET+FFR+++ A+ D++ Q +++ S +TGEE YS+AM Sbjct: 58 QALIELAVVPETWFFRDREAILAVARLASDKLAAQPA--AVIRILSLPCSTGEEPYSLAM 115 Query: 131 -LLDDQRSQHPWSVLASDISLRVLSFGRRALYP--MVRGELIPPPYLSRYCLRGTGEYEG 187 LLD + + + A DI R L LY RG+ + + R+ G++ Sbjct: 116 ALLDAGLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELA--FRDRHFDLEQGQWRL 173 Query: 188 HFLIDQKIRKRVTFEQRNLLSLPAEMDGQ-FDVVFLRNVIIYFDFPTKVDVLRSVVSKLR 246 H ++IR +V F NLL+ Q +D +F RNV+IYF+ + V++ + S ++ Sbjct: 174 H----ERIRSQVRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMK 229 Query: 247 PGGWLVVGHSES 258 P + VG +E Sbjct: 230 PDALIFVGPAEG 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 767,343 Number of extensions: 28410 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 5 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)