BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0168.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 448 e-127 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 312 4e-86 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 184 1e-47 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 142 6e-35 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 79 9e-16 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 448 bits (1153), Expect = e-127, Method: Compositional matrix adjust. Identities = 232/369 (62%), Positives = 282/369 (76%), Gaps = 10/369 (2%) Query: 15 RQVIQDLLEPCPDINVIATAPDPIFAMAKMRANWPDVIVLDIEMPRMDGITFLRQIMAIR 74 RQ++Q +L PDI VI TAPDP+FA+ KM+ WPDVIVLDI+MPRMDG++FLRQIMA R Sbjct: 19 RQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATR 78 Query: 75 PTPVVICSSLAERSARVTMEALAAGAVGIITKPQLGIRDFLQDSSDELIAAIRAAAHVRL 134 PTPV++CS+ + EALAAGAV +I +PQLG+RDFLQ + +EL +AIRAA R Sbjct: 79 PTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQQAVEELASAIRAAMQARE 138 Query: 135 -RHAPTAVNAANVVNAAGGVNFGNEAERNARHDAVAAGIVDGSRFTADAVLDPPGYLDGL 193 + AP A A G++ R HDA ++ R+TADAVL PPG DGL Sbjct: 139 GQRAPKA-------PARLGLSPVKAGAREMLHDAAWGAVL--QRYTADAVLQPPGPEDGL 189 Query: 194 IPVTPRIIAIGASTGGPQALEKVLRDLSVDCPGLIIVQHMPEKFTLSFAKRLNAISVIEV 253 P T RI+AIGASTGGPQALE+VL LS CPG+++VQHMP++FT SFA+RL+ IS EV Sbjct: 190 RPATARIVAIGASTGGPQALERVLGKLSQRCPGIVVVQHMPQRFTRSFAERLHRISGAEV 249 Query: 254 KEAEHHDLVLPGRALVAPGGSHVMVRRDGVQYYVEVVNGPLVSRHKPSVDVLFRSVAKTA 313 KEAEH D +LPGR L+APGG H++VRR+G+QYYVE ++GPLVSRHKPSVDVLFRS+AK A Sbjct: 250 KEAEHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAA 309 Query: 314 GKNAVGIIMTGMGDDGARGMKELRDAGASTYAQDESSCVVFGMPKEAVLQGGVSDVISLE 373 G NAVGIIMTGMGDDGARGM+EL D G +TYAQDE+S VVFGMPKEA+ QGGV DVI+LE Sbjct: 310 GSNAVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLE 369 Query: 374 TMSSVINHY 382 +S+VI Y Sbjct: 370 HISAVIEQY 378 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 312 bits (799), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 170/375 (45%), Positives = 243/375 (64%), Gaps = 34/375 (9%) Query: 1 MTIKVMIVDDSSVVRQVIQDLLEPCPDINVIATAPDPIFAMAKMRANWPDVIVLDIEMPR 60 M IKV+IVDDS+++R V+++++ PD+ V+ APDPI A ++ PDV+ LD+EMPR Sbjct: 1 MKIKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPR 60 Query: 61 MDGITFLRQIMAIRPTPVVICSSLAERSARVTMEALAAGAVGIITKPQLGIRDFLQDSSD 120 MDG+ FL ++M +RP PVV+ SSL ER + +T+ AL GA+ +TKP++ I+ +Q+ +D Sbjct: 61 MDGLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYAD 120 Query: 121 ELIAAIRAAAHVRLRHAPTAVNAANVVNAAGGVNFGNEAERNARHDAVAAGIVDGSRFTA 180 + IRAAA R+ A A + AAGG G+EA R+ + Sbjct: 121 MIADKIRAAAKARV----RARPARSAEQAAGG---GSEALPQIRNPLTS----------- 162 Query: 181 DAVLDPPGYLDGLIPVTPRIIAIGASTGGPQALEKVLRDLSVDCPGLIIVQHMPEKFTLS 240 + ++I IGASTGG +A+++ L + DCPG++I QHMPE FT S Sbjct: 163 ----------------SEKLIIIGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRS 206 Query: 241 FAKRLNAISVIEVKEAEHHDLVLPGRALVAPGGSHVMVRRDGVQYYVEVVNGPLVSRHKP 300 FA+RLN + I V E+ ++ VLPG A +APG SH+ + R G Y ++ GP V+RH+P Sbjct: 207 FAQRLNNLCKISVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRP 266 Query: 301 SVDVLFRSVAKTAGKNAVGIIMTGMGDDGARGMKELRDAGASTYAQDESSCVVFGMPKEA 360 SVDVLF+S A AGKNAVG+I+TGMG DGA+GM E+R+AGA +AQDE+SCVVFGMP+EA Sbjct: 267 SVDVLFQSAATCAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREA 326 Query: 361 VLQGGVSDVISLETM 375 + G +V++L+ M Sbjct: 327 IAVGAAHEVLALQAM 341 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 130/384 (33%), Positives = 200/384 (52%), Gaps = 39/384 (10%) Query: 3 IKVMIVDDSSVVRQVIQDLLEPCPDINVIATAPDPIFAMAKMRANWPDVIVLDIEMPRMD 62 IK++I+DDS+++R+ + + E + I + A+ R+ PDV+ LDI MP MD Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFD-ICLGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 63 GITFLRQIMAIRPTPVVICSSLAERSARVTMEALAAGAVGIITKPQLGIRDFLQDSSDEL 122 G++ L +M RP PVV+ SSL E+ A T EA+ GAV + KP I L + +L Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 123 IAAIRAAAHVRLRHAPTAVNAANVVNAAGGVNFGNEAERNARHDAVAAGIVDGSRFTADA 182 +A +R AA R+R A A + +AA + + +R A Sbjct: 121 LAKVRVAAKARIRSRAPAPELARA------------------REPLAAPVAEAARRGRPA 162 Query: 183 VLDPPGYLDGLIPVTPRIIAIGASTGGPQALEKVLRDLSVDCPGLIIV-QHMPEKFTLSF 241 DGL + IG STGGP LE++L L P I+V QHMP FT SF Sbjct: 163 S-------DGL-------VLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSF 208 Query: 242 AKRLNAISVIEVKEAEHHDLVLPGRALVAPGGSHVMVRRDGVQYYVEVVNGPLVSRH--K 299 A+R++ + + V E +V G +A GG+ ++V + G ++ V+ P H Sbjct: 209 AERMDKLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRG--SHLSVLPKPESPTHLWH 266 Query: 300 PSVDVLFRSVAKT-AGKNAVGIIMTGMGDDGARGMKELRDAGASTYAQDESSCVVFGMPK 358 PSV++L RS ++ A + +G+++TGMG+DGA E+ G T A+ ESSCVVFGMPK Sbjct: 267 PSVELLARSALQSMAARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPK 326 Query: 359 EAVLQGGVSDVISLETMSSVINHY 382 + + +GG S V+ +++ + + Sbjct: 327 DLIDRGGASVVLPASKIATQLRVW 350 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 142 bits (357), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 115/383 (30%), Positives = 182/383 (47%), Gaps = 50/383 (13%) Query: 3 IKVMIVDDSSVVRQVIQDLLEPCPDINVIATAPDPIFAMAKMRANWPDVIVLDIEMPRMD 62 +K+ IV+D+ ++ +V++ ++ +I A D A+ PDV+++D+ MP++D Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 63 GITFLRQIMAIRPTPVVICSSLAERSARVTMEALAAGAVGIITKPQL----GIRDFLQDS 118 G R+IM P P+++ +S + SA EA+ GA+ P L G RD Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRD----- 115 Query: 119 SDELIAAIRAAAHVRLRHAPTAVNAANVVNAAGGVNFGNEAERNARHDAVAAGIVDGSRF 178 + LI I R +P V+ R R + +A Sbjct: 116 AAALIEKIDNLG----RLSPEMVS---------------PPVRGGRAETLAPA------- 149 Query: 179 TADAVLDPPGYLDGLIPVTPRIIAIGASTGGPQALEKVLRDLSVDCPG-LIIVQHMPEKF 237 P D L P ++A+GAS GGP AL +L+ + G ++IVQH+ F Sbjct: 150 -------QPARADSL----PPLVAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAF 198 Query: 238 TLSFAKRLNAISVIEVKEAEHHDLVLPGRALVAPGGSHVMVRRDGVQYYVEVVNGPLVSR 297 A+ L S ++V A+ G L+A H++V+ G YVE G Sbjct: 199 APGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVKEGGRLGYVE---GAPNDV 255 Query: 298 HKPSVDVLFRSVAKTAGKNAVGIIMTGMGDDGARGMKELRDAGASTYAQDESSCVVFGMP 357 ++PSVDV F+S+ + G+++TGMG DGA G+K LRD+GA T AQD+ SC V+GMP Sbjct: 256 YRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMP 315 Query: 358 KEAVLQGGVSDVISLETMSSVIN 380 K AV +V+ + M++ + Sbjct: 316 KAAVALNAAVEVLPVTGMAARLQ 338 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 78.6 bits (192), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 56/184 (30%), Positives = 89/184 (48%), Gaps = 1/184 (0%) Query: 201 IAIGASTGGPQALEKVLRDLSVDCP-GLIIVQHMPEKFTLSFAKRLNAISVIEVKEAEHH 259 I IGAS GG +AL ++L L ++V H+P + A+ V EA+ Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 260 DLVLPGRALVAPGGSHVMVRRDGVQYYVEVVNGPLVSRHKPSVDVLFRSVAKTAGKNAVG 319 + G AP H++V R + + N LV+ +P++DVLF S A G +G Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 Query: 320 IIMTGMGDDGARGMKELRDAGASTYAQDESSCVVFGMPKEAVLQGGVSDVISLETMSSVI 379 I++TG DGA GM+ + AG T Q V MP +A+ G + V++L+ ++ ++ Sbjct: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIADLL 196 Query: 380 NHYG 383 G Sbjct: 197 RTLG 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,190,978 Number of extensions: 50129 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 9 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)