BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0167.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309962|ref|YP_003774054.1| response regulator [Herbaspiril... 549 e-157 gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 189 3e-49 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 175 8e-45 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 172 4e-44 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 172 6e-44 >gi|300309962|ref|YP_003774054.1| response regulator [Herbaspirillum seropedicae SmR1] Length = 420 Score = 549 bits (1414), Expect = e-157, Method: Compositional matrix adjust. Identities = 280/402 (69%), Positives = 343/402 (85%), Gaps = 1/402 (0%) Query: 6 IETLLIVDDSSLQRLHTVGLMRELGVEMIYEAGDGQEALDLLALLKLPPSLVIVDLEMPG 65 I++ L+VDDS++QR H+V L+++LG ++IYEAG+G EALDLL LLKLPPSL++VDLEMPG Sbjct: 10 IKSALVVDDSAVQRQHSVQLLQQLGADLIYEAGNGNEALDLLGLLKLPPSLMVVDLEMPG 69 Query: 66 MDGVEFIQHLQERKLNFPIIVASSRETSLLASVETMIEALGMHVLGALQKPLNQEKILGA 125 MDG+E IQ LQ++ ++ P+IVASSRETSLL SVETMI ALGM+++G LQKPLNQ ++ A Sbjct: 70 MDGIELIQQLQQKGIDVPLIVASSRETSLLLSVETMIHALGMNLIGVLQKPLNQHQLRAA 129 Query: 126 LKSFDGNLALPAKD-DALPSVCETELAAAIRNGDIVAFYQPKVDIRTGMLKGVEALARWM 184 L+SF + D D PS+CE +L AAI G ++ ++QPKVD++TG+LKGVEALARW+ Sbjct: 130 LESFRPHGTEHQPDHDPQPSMCEHDLGAAIEGGQVLPYFQPKVDVQTGILKGVEALARWI 189 Query: 185 HPTNGMVPPDRFIPLAEQSGLIHELTLSMLTQAMAQAALWNERGLPLKVAVNLSPLSLDI 244 HP GMV PDRFIPLAEQ GLIH LTL+M+ QAM QAA+W+ RGL +KVAVNLSPL L+ Sbjct: 190 HPERGMVSPDRFIPLAEQCGLIHPLTLAMMDQAMGQAAIWHSRGLAIKVAVNLSPLLLEK 249 Query: 245 PGFAQKIVDLLAQHDLQADQMVLEITEGSVVANLGMALGTLARLRLKGFGLSIDDYGTGF 304 P F +I++L+ +H L A+ +VLEITE SVVA+ G +LG LARLRLKGFGLSIDDYGTGF Sbjct: 250 PDFLHRILELVDKHALPAEHVVLEITESSVVAHKGNSLGMLARLRLKGFGLSIDDYGTGF 309 Query: 305 SSMQQLARIPFTELKIDRSFVHGAHSRQNLRVILQSALDMAKRLELVTVAEGIETIEDWR 364 SSMQQLARIPFTELKIDRSFVHGAH R+NLRVILQSALDMA+RLELVTVAEG+ET+EDWR Sbjct: 310 SSMQQLARIPFTELKIDRSFVHGAHDRKNLRVILQSALDMAQRLELVTVAEGVETMEDWR 369 Query: 365 LLQESGCSIGQGYLIARPMPANDLPVWLKGHHRRLGELRPLA 406 LLQ+ GCS+GQGYLI +PMPA++LP+WLKGHHRRL ELRP+A Sbjct: 370 LLQDFGCSLGQGYLIGKPMPAHELPLWLKGHHRRLHELRPVA 411 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 189 bits (480), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 98/239 (41%), Positives = 139/239 (58%) Query: 153 AIRNGDIVAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQSGLIHELTLS 212 A+ + + + QPK+++ +G + G EAL RW+HP G + PD FIP AE +G I LTL Sbjct: 541 ALERDEFMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLW 600 Query: 213 MLTQAMAQAALWNERGLPLKVAVNLSPLSLDIPGFAQKIVDLLAQHDLQADQMVLEITEG 272 M+ Q + ++G+ LK +VNLS L +L +H LQ + LEITE Sbjct: 601 MMEQVARLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITES 660 Query: 273 SVVANLGMALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFVHGAHSRQ 332 +++ + A TL RL GF LSIDD+GTG+SS+ L R+P ELKID+SFV Q Sbjct: 661 AIMDDPARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQ 720 Query: 333 NLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCSIGQGYLIARPMPANDLPVW 391 + I++S +D+ L L VAEG+E+ E W LL++ GC GQGY + +PMP W Sbjct: 721 DDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLAW 779 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 175 bits (443), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 97/254 (38%), Positives = 148/254 (58%), Gaps = 7/254 (2%) Query: 147 ETELAAAIRNGDIVAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQSGLI 206 E+ L A+R GD+ +QP++ + L GVEALARW HP G + P RFIP+AE+ GLI Sbjct: 733 ESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIPIAEECGLI 792 Query: 207 HELTLSMLTQAMAQAALWNERGLPL-KVAVNLSPLS---LDIPGFAQKIVDLLAQHDLQA 262 EL L +A Q + W RG+ + ++VNLS + LD+P F + LL ++ L+ Sbjct: 793 GELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQF---VTGLLRRYALEP 849 Query: 263 DQMVLEITEGSVVANLGMALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDR 322 + LEITE ++ T+A + G L++DD+GTG+SS+ L ++P +ELK+D+ Sbjct: 850 SDLTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQ 909 Query: 323 SFVHGAHSRQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCSIGQGYLIARP 382 SFV + + + + + + L+L VAEG+E E + LL GC + QGYL +RP Sbjct: 910 SFVRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRP 969 Query: 383 MPANDLPVWLKGHH 396 M A+ L W++ H Sbjct: 970 MSADALENWVRDLH 983 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 172 bits (436), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 98/246 (39%), Positives = 138/246 (56%), Gaps = 6/246 (2%) Query: 150 LAAAIRNGDIVAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQSGLIHEL 209 L A+ + +YQPK+ ++ G L G EAL RW P GMVPPDRFIP AE++GLI + Sbjct: 446 LRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPI 505 Query: 210 TLSMLTQAMAQAALWNERGLP-LKVAVNLSPLSLD--IPGFAQKIVDLLAQHDLQADQMV 266 +L A Q W + GLP + VAVNLSP L+ +P F + +LAQ L A + Sbjct: 506 GEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQSLPDF---VAGVLAQSGLAASWLE 562 Query: 267 LEITEGSVVANLGMALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSFVH 326 LEITE V+ + ++ TL L+ G +S+DD+GTG+SS+ L R P LKID+SFV Sbjct: 563 LEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVR 622 Query: 327 GAHSRQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCSIGQGYLIARPMPAN 386 + I+++ + +A L L +AEG+E E L+E+ C QGY +PM Sbjct: 623 DIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEVA 682 Query: 387 DLPVWL 392 W+ Sbjct: 683 VFTAWM 688 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 172 bits (435), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 90/244 (36%), Positives = 145/244 (59%), Gaps = 3/244 (1%) Query: 148 TELAAAIRNGDIVAFYQPKVDIRTGMLKGVEALARWMHPTNGMVPPDRFIPLAEQSGLIH 207 +L A++ G +QP +++++G++ EAL RW HP GM+ PDRFIPLAE+SGLI Sbjct: 490 NDLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGLIF 549 Query: 208 ELTLSMLTQAMAQAALWNERGL-PLK--VAVNLSPLSLDIPGFAQKIVDLLAQHDLQADQ 264 +L +L +A + WN +G P + ++VNLSP+ + ++D L+ Q Sbjct: 550 DLGRWILQEAARVSVRWNAQGTSPFRKQISVNLSPVQFLNGHYDAIVMDQLSLQGCQPGL 609 Query: 265 MVLEITEGSVVANLGMALGTLARLRLKGFGLSIDDYGTGFSSMQQLARIPFTELKIDRSF 324 + +EITEG ++ + L LR G +++DD+GTG+S+M L + LKIDRSF Sbjct: 610 IAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMDYLKIDRSF 669 Query: 325 VHGAHSRQNLRVILQSALDMAKRLELVTVAEGIETIEDWRLLQESGCSIGQGYLIARPMP 384 V + + I+++ + MA RL + +AEG+ET+ +LL+++GC QGYL +RP+P Sbjct: 670 VQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRPLP 729 Query: 385 ANDL 388 +D Sbjct: 730 EDDF 733 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,276,718 Number of extensions: 52789 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 20 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)