BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0166.p (157 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309963|ref|YP_003774055.1| 2-amino-4-hydroxy-6-hydroxymeth... 201 2e-53 >gi|300309963|ref|YP_003774055.1| 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Herbaspirillum seropedicae SmR1] Length = 161 Score = 201 bits (511), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 107/151 (70%), Positives = 117/151 (77%) Query: 3 AFIGIGGNLGDARTTVEDALRRLALLPDTTLSAQSHLYRTAPVDAGGDDYVNAVAQLDTT 62 A+IGIGGNLGDAR TVE A+ RLA LP T L S LYRTAPVDA GDDYVNAVA + TT Sbjct: 8 AWIGIGGNLGDARATVETAIARLAALPQTQLLRSSSLYRTAPVDASGDDYVNAVALVSTT 67 Query: 63 LTPHALLAALQAIELAHGRERPYRNAPRTLDLDILLYGELQLADDVLTLPHPRMAQRAFV 122 L LL ALQAIEL HGRERPYRNAPRTLDLD+L+YGE ++AD LT+PHPRM QRAFV Sbjct: 68 LPALDLLHALQAIELQHGRERPYRNAPRTLDLDVLMYGEARIADQELTVPHPRMVQRAFV 127 Query: 123 LVPLSEIAPDASIPGVGATRDLLVQVRDQAI 153 L PL EI +A IPGVGA R LL + DQ I Sbjct: 128 LAPLLEIDENAVIPGVGAARPLLDAITDQPI 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 462,338 Number of extensions: 16403 Number of successful extensions: 57 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 57 Number of HSP's successfully gapped: 1 Length of query: 157 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 79 Effective length of database: 1,250,540 Effective search space: 98792660 Effective search space used: 98792660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)