BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0157.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309971|ref|YP_003774063.1| hypothetical protein Hsero_0636... 889 0.0 gi|300309976|ref|YP_003774068.1| hypothetical protein Hsero_0641... 265 5e-72 >gi|300309971|ref|YP_003774063.1| hypothetical protein Hsero_0636 [Herbaspirillum seropedicae SmR1] Length = 475 Score = 889 bits (2296), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/475 (89%), Positives = 451/475 (94%), Gaps = 4/475 (0%) Query: 1 MANFFDEMYSDGT----VSVRKHYDEFAAWLSSQTAETIVRKRAESDLIFRRVGITFAVY 56 MA+FFDEMYS + VR+HY EFA WLS+Q+AETI RKRAESDLIFRRVGITFAVY Sbjct: 1 MAHFFDEMYSGESHGNEAQVRQHYAEFANWLSAQSAETIARKRAESDLIFRRVGITFAVY 60 Query: 57 GNDAGTERLIPFDIIPRIIHAAEWAKLEAGLVQRVKALNMFIHDIYHGQKIIKAGVIPAE 116 GNDAGTERLIPFDIIPRIIH AEWA+LEAGLVQRV+ALNMFIHDIYH QKI+KAG+IP E Sbjct: 61 GNDAGTERLIPFDIIPRIIHKAEWARLEAGLVQRVRALNMFIHDIYHEQKIVKAGIIPPE 120 Query: 117 QVLKNAQYRPEMQDVDVASDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM 176 Q+ +NAQYR EMQDV+V +DIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM Sbjct: 121 QIFQNAQYRREMQDVNVVNDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM 180 Query: 177 MMRLFPELFTRHKIAPVDHYPDMLLDNLRSVAPVGVVDPTVVVMTPGMYNSAYFEHAFLA 236 MMRLFP+LFTRHKIAPVDHYPD+LLDNLRSVAP GV DPTVVVMTPGMYNSAYFEHAFLA Sbjct: 181 MMRLFPDLFTRHKIAPVDHYPDLLLDNLRSVAPAGVTDPTVVVMTPGMYNSAYFEHAFLA 240 Query: 237 QQMGVELVEGQDLFVKDDTVYMRTTRGPKRVDVIYRRIDDDYLDPLAFRADSSLGVPGLL 296 QQMGVELVEGQDLFVKD+ VYMRTTRGPKRVDVIYRRIDDD+LDPLAFR DS+LGVPGLL Sbjct: 241 QQMGVELVEGQDLFVKDNVVYMRTTRGPKRVDVIYRRIDDDFLDPLAFRPDSALGVPGLL 300 Query: 297 SAYRAGKVTLTNAIGTGVADDKSIYPYVPDMIKFYLSEEPILNNVPTYQCRKKEDLSYTL 356 AYRAGKVTLTNAIGTGVADDKSIYPYVPDM++FYLSE+PILNNVPTYQCRKKEDLSYTL Sbjct: 301 QAYRAGKVTLTNAIGTGVADDKSIYPYVPDMVRFYLSEDPILNNVPTYQCRKKEDLSYTL 360 Query: 357 ANLEKLVVKEVHGAGGYGMLVGPASTRQEIEDFRQRVIAKPDGYIAQPTLALSVCPTYVD 416 ANLEKLVVKEVHGAGGYGMLVGPASTRQEIEDFR R+IAKPDGYIAQPTLALSVCPTYV+ Sbjct: 361 ANLEKLVVKEVHGAGGYGMLVGPASTRQEIEDFRARIIAKPDGYIAQPTLALSVCPTYVE 420 Query: 417 AGIAPRHLDLRPFVLSGKTVSMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK 471 +GIAPRHLDLRPFVLSGK ++MVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK Sbjct: 421 SGIAPRHLDLRPFVLSGKQITMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK 475 >gi|300309976|ref|YP_003774068.1| hypothetical protein Hsero_0641 [Herbaspirillum seropedicae SmR1] Length = 833 Score = 265 bits (678), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 158/477 (33%), Positives = 250/477 (52%), Gaps = 16/477 (3%) Query: 5 FDEMYS-DGTVSVRKHYDEFAAWLSSQTAETIVRKRAESDLIFRRVGITFAVYGNDAGTE 63 +DEM + DG +R H+ L + + + + R+ E G+T+ VY + G Sbjct: 16 YDEMLAADG--RLRPHWRTLIDQLENLSPDMLRRRANEVRDAIASDGVTYNVYADPKGAN 73 Query: 64 RLIPFDIIPRIIHAAEWAKLEAGLVQRVKALNMFIHDIYHGQKIIKAGVIPAEQVLKNAQ 123 R D++P ++ + EW LE + QR + +N + DIY Q +I G++P V Sbjct: 74 RPWELDLLPHLVSSEEWDFLERAVGQRARLMNGLLSDIYGPQTLIAEGLLPPALVFGQHG 133 Query: 124 YRPEMQDVDVASDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKMMMRLFPE 183 Y ++ H +D+ R+ +G ++V+ D + PSG Y L+NR++M R PE Sbjct: 134 YLMPCHGWQPPGGVHLHAYAIDLARSVDGTWWVVSDRTQGPSGTGYALQNRQIMARAMPE 193 Query: 184 LFTRHKIAPVDHYPDMLLDNLRSVAPVGVVDPTVVVMTPGMYNSAYFEHAFLAQQMGVEL 243 + Y L L +AP P +V++TPG YN Y EHAFLA +G L Sbjct: 194 ALRDMHVESPHRYFHTLRAMLTRLAPAQGEVPLIVLLTPGPYNETYSEHAFLAHTLGFPL 253 Query: 244 VEGQDLFVKDDTVYMRTTRGPKRVDVIYRRIDDDYLDPLAFRADSSLGVPGLLSAYRAGK 303 VEG DL V+ + V+++T G +RV I RR+DDDY DPL RADS+LGVPGL A R G Sbjct: 254 VEGVDLTVRGEQVFLKTLNGLRRVHAILRRMDDDYCDPLELRADSALGVPGLTQAARLGN 313 Query: 304 VTLTNAIGTGVADDKSIYPYVPDMIKFYLSEEPILNNVPTYQCRKKEDLSYTLANLEKLV 363 V + N++G+GV + +++ ++P + + E +L +V ++ C +K L YTLA+ ++LV Sbjct: 314 VLIANSLGSGVLESTALHGFLPAINERLYGEPLLLPSVASWWCGEKPALDYTLAHFDELV 373 Query: 364 V----KEVHGAGGYGMLVGPASTRQEIEDFRQRVIAKPDGYIAQPTLALSVCPTYVDAG- 418 + + +G V A+ R+ IE + +P Y AQ + LS P +G Sbjct: 374 IVPAFSSMRMQPVFGHSVTGAARRRLIESLQ----LQPHAYAAQEWVRLSQAPVMSRSGD 429 Query: 419 --IAPRHLDLRPFVLSGKT--VSMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK 471 + R + LR F ++ ++ GGLTRVA ++ VV+ QGG +KD W+L + Sbjct: 430 YRLMNRTISLRVFAVADGEGGYQVMPGGLTRVAPRQRRDVVSMQQGGTSKDVWVLRE 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,407,514 Number of extensions: 56355 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 2 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)