BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0009.0153.p
         (208 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depo...   368   e-103
gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] d...   210   5e-56
gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet...    61   6e-11

>gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate
           depolymerase [Herbaspirillum seropedicae SmR1]
          Length = 408

 Score =  368 bits (945), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 169/208 (81%), Positives = 189/208 (90%)

Query: 1   MTLMAGPLDTRVNPTTVNELAKSKPIEWFEKNLVSTVPARHPGGGRKVYPGFMQLAAFMN 60
           MTLMAGP+DTRVNPT VN LAKSKPI WFEKN++STVPARH G GR+VYPGF+QLAAFMN
Sbjct: 201 MTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPARHAGAGRRVYPGFVQLAAFMN 260

Query: 61  MNLNRHVDAFRNLYTYLIEEEHDKAEAIKVFYEEYFAMSDLPAEFYLETVRVVFQEHALP 120
           MNL+RHV+AF  LY +L++ EH +A+ IK FYEEYFAM+DLPAEFYLETVR VFQEHALP
Sbjct: 261 MNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMADLPAEFYLETVRTVFQEHALP 320

Query: 121 LGLLQFQGRPVNLKAIRRTALFTVEGEKDDICAVGQTVAAQDMCSSIRPYMRLHHVQTAV 180
           LG L + GRPV  +AIRRTALFT+EGEKDDICAVGQT+AAQ++CS IRPYMRLHHVQTAV
Sbjct: 321 LGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAAQELCSGIRPYMRLHHVQTAV 380

Query: 181 GHYGVFNGRRWENEIYPRLRDFINMHHR 208
           GHYGVFNGRRW+NEIYPRLRDFINMHHR
Sbjct: 381 GHYGVFNGRRWDNEIYPRLRDFINMHHR 408


>gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate]
           depolymerase [Herbaspirillum seropedicae SmR1]
          Length = 411

 Score =  210 bits (535), Expect = 5e-56,   Method: Compositional matrix adjust.
 Identities = 98/203 (48%), Positives = 135/203 (66%)

Query: 1   MTLMAGPLDTRVNPTTVNELAKSKPIEWFEKNLVSTVPARHPGGGRKVYPGFMQLAAFMN 60
           MT+M GP+D R +PT VN+LA  KP  WFE  ++ +VPA +PG GRKVYPGF+Q A F+ 
Sbjct: 205 MTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVPANYPGFGRKVYPGFLQHAGFVA 264

Query: 61  MNLNRHVDAFRNLYTYLIEEEHDKAEAIKVFYEEYFAMSDLPAEFYLETVRVVFQEHALP 120
           MN  RH  +  + Y +L + +   AE  + FY+EY A+ D+PAEFYLET++VVFQ+  L 
Sbjct: 265 MNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAVLDMPAEFYLETIKVVFQDFNLA 324

Query: 121 LGLLQFQGRPVNLKAIRRTALFTVEGEKDDICAVGQTVAAQDMCSSIRPYMRLHHVQTAV 180
            G  + +G+ V  + I+  ALFT+EGE DDI   GQT AAQ++CSSI    + H      
Sbjct: 325 RGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQAAQELCSSIPKARKQHFTVPKA 384

Query: 181 GHYGVFNGRRWENEIYPRLRDFI 203
           GHYG+F+GRRW   + P++ +FI
Sbjct: 385 GHYGIFSGRRWREIVCPKIGEFI 407


>gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase
           [Herbaspirillum seropedicae SmR1]
          Length = 376

 Score = 61.2 bits (147), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 50/205 (24%), Positives = 95/205 (46%), Gaps = 17/205 (8%)

Query: 1   MTLMAGPLDTRVNPTTVNELAKSKPIEWFEKNLVSTVPARHPGGGRKVYPGFMQLAAFMN 60
           + L   P+D       + ++ +  P+ ++E +LV +      GGG  +  G   L  + N
Sbjct: 183 LVLAGSPIDAGAGEGPLKQMVQRLPLRFYE-DLVRS------GGG--LMRGSFMLQGWKN 233

Query: 61  MNLNRHVDAFRNLYTYLIEEEHDKAEAIK--VFYEEYFAMSDLPAEFYLETVRVVFQEHA 118
           M+ + H   + N +  L++   D A   K   F   Y    DLP  +YL+ +  +F+++ 
Sbjct: 234 MHPDEH---YFNEHLELLQHIDDPAYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNL 290

Query: 119 LPLGLLQFQGRPVNLKAIRRTALFTVEGEKDDICAVGQTVAAQDMCSSIRPYMRLHHVQT 178
           L  G     G+ ++L+ I    L+ + GE+DDI    Q + A  +  +  P  ++     
Sbjct: 291 LARGKYVALGQQLDLRQI-TCPLYLLAGERDDITTPEQVLNAAALVGT--PAAQITQKIV 347

Query: 179 AVGHYGVFNGRRWENEIYPRLRDFI 203
             GH G+F G R   E++P++  ++
Sbjct: 348 PGGHIGLFMGSRTLAEVWPQIATWL 372


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.138    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 658,813
Number of extensions: 25896
Number of successful extensions: 69
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 69
Number of HSP's successfully gapped: 3
Length of query: 208
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 127
Effective length of database: 1,236,335
Effective search space: 157014545
Effective search space used: 157014545
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 128 (53.9 bits)