BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0131.p (78 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspi... 134 2e-33 gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspi... 108 6e-26 >gi|300309983|ref|YP_003774075.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 242 Score = 134 bits (336), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 61/77 (79%), Positives = 68/77 (88%) Query: 1 MQLTERGASANHTDVVSLDADMLGVETVGNDYLASVKFFGFIKEDQNASPTQFNEIWNLS 60 +Q+ ERGA++N TDVVSLDA++LGVETVGNDYLASVKFFGFIKED ASP QF EIWNLS Sbjct: 161 LQIQERGAASNQTDVVSLDAEVLGVETVGNDYLASVKFFGFIKEDPTASPAQFAEIWNLS 220 Query: 61 KPVTGNGGWVLAGIQQL 77 KP G GGWVLAGIQQ+ Sbjct: 221 KPAAGQGGWVLAGIQQI 237 >gi|300313191|ref|YP_003777283.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 108 bits (271), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 51/77 (66%), Positives = 61/77 (79%) Query: 1 MQLTERGASANHTDVVSLDADMLGVETVGNDYLASVKFFGFIKEDQNASPTQFNEIWNLS 60 MQLTERGA+ N TDVV LDA +LG+ET+ DYLA+V+F G IKE + S F EIWNLS Sbjct: 241 MQLTERGAAPNATDVVQLDAQLLGIETMAFDYLATVRFQGLIKEAPDVSAEPFAEIWNLS 300 Query: 61 KPVTGNGGWVLAGIQQL 77 KP+ G+GGWVLAGIQQ+ Sbjct: 301 KPLNGSGGWVLAGIQQV 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.131 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 250,612 Number of extensions: 8397 Number of successful extensions: 9 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 9 Number of HSP's successfully gapped: 2 Length of query: 78 Length of database: 1,619,870 Length adjustment: 49 Effective length of query: 29 Effective length of database: 1,387,855 Effective search space: 40247795 Effective search space used: 40247795 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 123 (52.0 bits)