BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0127.p (155 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 245 1e-66 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 149 1e-37 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 128 2e-31 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 127 3e-31 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 85 2e-18 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 245 bits (625), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 119/149 (79%), Positives = 135/149 (90%) Query: 1 VRADSXSPSAQAIIVRADSPIRTVADLKRKKIAVTKAAGVHYLLIATLEKAGLKFSDIDA 60 V +S SP+AQAI+VRADS +R +DLK KKIAVTKAAGVHYLLIATLEKAGLKFSD+DA Sbjct: 114 VAQESPSPAAQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDA 173 Query: 61 AYLTPADGRAAFERGSVDAWVTWDPFLAGVQKQTHVRILSDGRNVADYQRYYLASTPFAE 120 AYL+PADGRAAFERGSVDAWVTWDPFLAGVQ+Q+ VRILSDGR VADYQRYYLAS FA+ Sbjct: 174 AYLSPADGRAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQ 233 Query: 121 AHPDVLAIVFDELKKTGLWVRRNPKEAAA 149 A P+VL +VFDEL+KTG WV+++PKEAAA Sbjct: 234 ARPEVLRVVFDELQKTGHWVKQHPKEAAA 262 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 149 bits (376), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 68/143 (47%), Positives = 101/143 (70%) Query: 7 SPSAQAIIVRADSPIRTVADLKRKKIAVTKAAGVHYLLIATLEKAGLKFSDIDAAYLTPA 66 +P A+ ++V+ +SPI++VA+LK KKIA K + VH+L++A L+KAGL++SDI YL PA Sbjct: 125 TPDAEGLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPA 184 Query: 67 DGRAAFERGSVDAWVTWDPFLAGVQKQTHVRILSDGRNVADYQRYYLASTPFAEAHPDVL 126 D RAAFERG+VDAW WDPF A +KQ R L+ G ++ +++L++ PFA +P V Sbjct: 185 DARAAFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVE 244 Query: 127 AIVFDELKKTGLWVRRNPKEAAA 149 I+ +E+ + G WVR + +AAA Sbjct: 245 KILLEEISREGEWVRTHTAQAAA 267 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 128 bits (322), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 66/147 (44%), Positives = 92/147 (62%), Gaps = 1/147 (0%) Query: 3 ADSXSPSAQAIIVRADSPIRTVADLKRKKIAVTKAAGVHYLLIATLEKAGLKFSDIDAAY 62 A+ P I V +SPIR+VADLK KK+A + V +LL+ LE+ GL + DI+ Y Sbjct: 112 AEQPYPKGIGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVY 171 Query: 63 LT-PADGRAAFERGSVDAWVTWDPFLAGVQKQTHVRILSDGRNVADYQRYYLASTPFAEA 121 +T AD RAAF+ G+VDA WDPFLAG + T RIL DG +++ + ++LA F + Sbjct: 172 VTNAADVRAAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDR 231 Query: 122 HPDVLAIVFDELKKTGLWVRRNPKEAA 148 H D++ VF ELKKT W + +P+E A Sbjct: 232 HKDIVRTVFAELKKTNQWTQSHPQETA 258 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 127 bits (320), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 63/149 (42%), Positives = 89/149 (59%) Query: 1 VRADSXSPSAQAIIVRADSPIRTVADLKRKKIAVTKAAGVHYLLIATLEKAGLKFSDIDA 60 V A P +A++V+ DS ++ LK K+IA+ K + VHY L+ LEK GLK+ D+ Sbjct: 125 VGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSNVHYFLVKLLEKHGLKYGDVTP 184 Query: 61 AYLTPADGRAAFERGSVDAWVTWDPFLAGVQKQTHVRILSDGRNVADYQRYYLASTPFAE 120 +L PAD RAAFERG+VDAW W+P+LA Q+ RIL+D V + YY ++ +A Sbjct: 185 VWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARILADADGVVKNRAYYFSTRDYAR 244 Query: 121 AHPDVLAIVFDELKKTGLWVRRNPKEAAA 149 H D+L I +EL K W + P E A+ Sbjct: 245 DHADLLRIALEELGKIDAWAQARPDEYAS 273 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 85.1 bits (209), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 50/142 (35%), Positives = 74/142 (52%), Gaps = 1/142 (0%) Query: 8 PSAQAIIVRADSPIRTVADLKRKKIAVTKAAGVHYLLIATLEKAGLKFSDIDAAYLTPAD 67 P A+ V +SP++++ +LK KKIA TK + L+ L + GLK SD++ +L AD Sbjct: 115 PEWTALAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHAD 174 Query: 68 GRAAFERGSVDAWVTWDPFLAGVQKQTHVRILSDGRNVADYQRYYLASTPFAEAHPDVLA 127 GRAA E+G V AW DP LA + + R++ N Y + S FA HPD + Sbjct: 175 GRAALEQGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYG-FLNVSDSFAARHPDQVK 233 Query: 128 IVFDELKKTGLWVRRNPKEAAA 149 V ++ W+ +P E A Sbjct: 234 RVIAAYERARSWIIAHPDETVA 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 508,818 Number of extensions: 20460 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 6 Length of query: 155 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 77 Effective length of database: 1,250,540 Effective search space: 96291580 Effective search space used: 96291580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)