BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0115.p (328 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 517 e-148 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 128 5e-31 gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 122 4e-29 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 107 1e-24 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 103 2e-23 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 517 bits (1331), Expect = e-148, Method: Compositional matrix adjust. Identities = 261/327 (79%), Positives = 292/327 (89%), Gaps = 1/327 (0%) Query: 1 MQRRHFLQLSTATVGAAALG-LPLTGRAADAPKTIRLDYATYSPPSLVLKKFGWLEEDLK 59 M RR FL+L+ A +G ALG +PL AA PK IRLDYA YSPPSLVL+KFGWLEEDLK Sbjct: 1 MHRRDFLRLAPAALGVTALGGIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 60 SSGGSVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPAKAVYVFSRPEWTAL 119 + G VKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNP KAVY++SRPEWTAL Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSRPEWTAL 120 Query: 120 AVQKDSTIRSVKELKGKKIAATKGTDPYLFLLRALHENGLAKSDVEILHLQHPDGRVALE 179 AV K+S ++S+ ELKGKKIAATKGTDPYLFLLRALHENGL KSDVEI+HLQH DGR ALE Sbjct: 121 AVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQHADGRAALE 180 Query: 180 QKRVDAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAAKYPDEVKRVIRAYE 239 Q RV AWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAA++PD+VKRVI AYE Sbjct: 181 QGRVAAWAGLDPHLAASELEAGSRLIYRNVNFNTYGFLNVSDSFAARHPDQVKRVIAAYE 240 Query: 240 RARLWIIKNPDETAKLIAEEARLSLPVAQLQLKRNDFSNPQIGKEHIDALRAAAPILLDE 299 RAR WII +PDET L++EE++LS PVA+LQLKRNDFS PQIG+EHI+ALRAAAPILL+E Sbjct: 241 RARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAPILLEE 300 Query: 300 ELVRSGTDLSRTITALIDPAYSQGLIR 326 +LV+SGTDL RTI ALIDP+Y+QG++R Sbjct: 301 DLVKSGTDLPRTINALIDPSYAQGVVR 327 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 93/274 (33%), Positives = 136/274 (49%), Gaps = 2/274 (0%) Query: 2 QRRHFLQLSTATVGAAALGLPLTGRAADAPKTIRLDYATYSPPSLVLKKFGWLEEDLKSS 61 +RR +LS +GA + L + A K +R+ Y + ++LK G LE+ L+ Sbjct: 13 RRRAIQRLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLKAHGTLEKRLQPL 72 Query: 62 GGSVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANG-NPAKAVYVFSRPEWTALA 120 G VKW + LE LN SVDFG + A+A G + Y P+ L Sbjct: 73 GVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAEGLL 132 Query: 121 VQKDSTIRSVKELKGKKIAATKGTDPYLFLLRALHENGLAKSDVEILHLQHPDGRVALEQ 180 VQK+S I+SV ELKGKKIA KG+D + ++ L + GL SD++ ++L D R A E+ Sbjct: 133 VQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLAPADARAAFER 192 Query: 181 KRVDAWAGLDPHLAASELEAGSRLIYRNVN-FNTYGFLNVSDSFAAKYPDEVKRVIRAYE 239 VDAWA DP AA+E + G+R + V N + F + FAAK P K ++ Sbjct: 193 GAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILLEEIS 252 Query: 240 RARLWIIKNPDETAKLIAEEARLSLPVAQLQLKR 273 R W+ + + A +A L + + LKR Sbjct: 253 REGEWVRTHTAQAAAQLAPIQGLDADIIETGLKR 286 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 122 bits (306), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 93/282 (32%), Positives = 145/282 (51%), Gaps = 12/282 (4%) Query: 2 QRRHFLQLSTATVGAAALGLPLTGRAADAPKTIRLDYATYSPPSLVLKKFGWLEEDLKSS 61 +RR LQ A GA A G PL AAD K +R+ Y ++ + +LK G LE+ L+ Sbjct: 9 KRRLLLQGLAA--GALASGTPLVRAAAD--KVLRIGYQKFNTLN-ILKGSGKLEQALQPQ 63 Query: 62 GGSVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPAKAVYVFSR---PEWTA 118 G SVKW + E LN++++D G A ++ A A+G AVY+ + P+ Sbjct: 64 GWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASG--VNAVYLGAEQPYPKGIG 121 Query: 119 LAVQKDSTIRSVKELKGKKIAATKGTDPYLFLLRALHENGLAKSDVEILHLQH-PDGRVA 177 + V ++S IRSV +LKGKK+A +G + LL+AL E GL+ D+E +++ + D R A Sbjct: 122 IFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRAA 181 Query: 178 LEQKRVDAWAGLDPHLAASELEAGSRLIYRNVNF-NTYGFLNVSDSFAAKYPDEVKRVIR 236 + VDA DP LA EL R++ N F SF ++ D V+ V Sbjct: 182 FQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVFA 241 Query: 237 AYERARLWIIKNPDETAKLIAEEARLSLPVAQLQLKRNDFSN 278 ++ W +P ETA+L+A + + V +L +R +++ Sbjct: 242 ELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTT 283 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 84/280 (30%), Positives = 137/280 (48%), Gaps = 10/280 (3%) Query: 28 ADAPKTIRLDYATYSPPSLVLKKFGWLEEDLKSSGGSVKWVLSQGSNRALEYLNSDSVDF 87 A AP+ +R+ Y + +++ G LE+ L G +VKWV LE L + S+DF Sbjct: 47 AAAPRQLRVGYQKGA--LSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSIDF 104 Query: 88 GSTAGLAALLARANGNPAKAVYV---FSRPEWTALAVQKDSTIRSVKELKGKKIAATKGT 144 G + A+A G P VYV RP A+ V+ DS ++ ++LKGK+IA KG+ Sbjct: 105 GDVGEAPPIFAQAAGTPL--VYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGS 162 Query: 145 DPYLFLLRALHENGLAKSDVEILHLQHPDGRVALEQKRVDAWAGLDPHLAASELEAGSRL 204 + + FL++ L ++GL DV + L D R A E+ VDAWA +P+LAA++ +R+ Sbjct: 163 NVHYFLVKLLEKHGLKYGDVTPVWLAPADARAAFERGAVDAWAIWEPYLAAAQQGLKARI 222 Query: 205 I--YRNVNFNTYGFLNVSDSFAAKYPDEVKRVIRAYERARLWIIKNPDETAKLIAEEARL 262 + V N + + D +A + D ++ + + W PDE A +A L Sbjct: 223 LADADGVVKNRAYYFSTRD-YARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGL 281 Query: 263 SLPVAQLQLKRNDFSNPQIGKEHIDALRAAAPILLDEELV 302 V + ++R + I + +D + A LD L+ Sbjct: 282 PRSVLDVVVRRYHYGTLPITRAILDEQQQIADTFLDLRLI 321 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 85/261 (32%), Positives = 121/261 (46%), Gaps = 11/261 (4%) Query: 3 RRHFLQLSTAT-VGAAALGLPLTGRAADAPKTIRLDYATYSPPSLVLKKFGWLEEDLKSS 61 RR L +TA +G+A L A K IR+ Y S +LK G LE+ L + Sbjct: 10 RRRLLHAATALGLGSA---LAPASVFAQGNKAIRIGYQKSSTLLTILKANGTLEKLLAPT 66 Query: 62 GGSVKWVLSQGSNRALEYLNSDSVDFGS-TAGLAALLARANGNPAKAVYVFSR---PEWT 117 G + W LE LN VD + A + A+A G AK YV P Sbjct: 67 GAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAG--AKLAYVAQESPSPAAQ 124 Query: 118 ALAVQKDSTIRSVKELKGKKIAATKGTDPYLFLLRALHENGLAKSDVEILHLQHPDGRVA 177 A+ V+ DS +R +LKGKKIA TK + L+ L + GL SDV+ +L DGR A Sbjct: 125 AIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSPADGRAA 184 Query: 178 LEQKRVDAWAGLDPHLAASELEAGSRLIYRNVNFNTYG-FLNVSDSFAAKYPDEVKRVIR 236 E+ VDAW DP LA + ++ R++ Y + S SFA P+ ++ V Sbjct: 185 FERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPEVLRVVFD 244 Query: 237 AYERARLWIIKNPDETAKLIA 257 ++ W+ ++P E A +A Sbjct: 245 ELQKTGHWVKQHPKEAAAFLA 265 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 983,350 Number of extensions: 38944 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 7 Length of query: 328 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 242 Effective length of database: 1,212,660 Effective search space: 293463720 Effective search space used: 293463720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)