BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0074.p (244 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310025|ref|YP_003774117.1| biopolymer transport transmembr... 444 e-126 gi|300312127|ref|YP_003776219.1| biopolymer transport transmembr... 211 5e-56 gi|300309940|ref|YP_003774032.1| biopolymer transport transmembr... 197 9e-52 gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel f... 80 1e-16 >gi|300310025|ref|YP_003774117.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 444 bits (1142), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/244 (90%), Positives = 231/244 (94%), Gaps = 1/244 (0%) Query: 1 MEVSPYGLESLWAQGDFVTKGVAVLLVAMSIASWYVIVTKALQLLRFRRASDAAGHAFWD 60 MEVSPYGLESLWAQGDFVTKGVAVLL+AMSIASWYVI+TKALQLLR R+AS AAGH FWD Sbjct: 1 MEVSPYGLESLWAQGDFVTKGVAVLLLAMSIASWYVIITKALQLLRLRKASAAAGHQFWD 60 Query: 61 TTSLPEGVATLGNDNPFAEVAKAGVTSMSHHTAHKGHLHDQLSVSDWVTLSLRQAIDEAS 120 TT+LPEGVATLG DNPFAEVAKAGVT+MSHHTAHKGHLHDQLSVSDWVTLSLRQAIDEAS Sbjct: 61 TTNLPEGVATLGQDNPFAEVAKAGVTAMSHHTAHKGHLHDQLSVSDWVTLSLRQAIDEAS 120 Query: 121 GKLQTGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTSGQASIDKVAGPVGEALIMTAL 180 GKLQ GMAVLASVGSTAPFVGLFGTVWGIYHALVSIGT+GQASIDKVAGPVGEALIMTAL Sbjct: 121 GKLQAGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTTGQASIDKVAGPVGEALIMTAL 180 Query: 181 GLAVAIPATFGYNALVRGNKTTIAKLNKFGFDLHALFVTGARSATEDAETGATKSAKLTA 240 GLAVAIPATFGYNALVRGNKT +AKLNKFGFDLHALFVTGAR+A ED + K AKLTA Sbjct: 181 GLAVAIPATFGYNALVRGNKTILAKLNKFGFDLHALFVTGARAAGEDGQPNGGK-AKLTA 239 Query: 241 VGRA 244 VGRA Sbjct: 240 VGRA 243 >gi|300312127|ref|YP_003776219.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 211 bits (537), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 109/230 (47%), Positives = 146/230 (63%), Gaps = 6/230 (2%) Query: 6 YGLESLWAQGDFVTKGVAVLLVAMSIASWYVIVTKALQLLRFRRASDAAGHAFWDTTSLP 65 YGL ++W QGD VT+ + +L+ MS SW V++ K + R R A FW S+ Sbjct: 4 YGLANVWLQGDVVTRFLITILLGMSSVSWIVMIVKGMANRRLSRIGLKAHKEFWAAHSIE 63 Query: 66 EGVATLG--NDNPFAEVAKAGVTSMSHHTAHKGHLHDQLSVSDWVTLSLRQAIDEASGKL 123 G+A +G DNP++ +A A V + + + L S ++WV +R +DE L Sbjct: 64 GGIAAIGAGTDNPYSNIATAAVEAQAQKD--QPLLEGSTSNTEWVAYCVRNELDEQIAHL 121 Query: 124 QTGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTSGQASIDKVAGPVGEALIMTALGLA 183 Q G+AVLAS+GST+PF+GLFGTVWGIYHAL++IG SGQAS+D VAGPVGE+L+MTA+GL Sbjct: 122 QGGLAVLASIGSTSPFIGLFGTVWGIYHALIAIGASGQASLDHVAGPVGESLVMTAVGLF 181 Query: 184 VAIPATFGYNALVRGNKTTIAKLNKFGFDLHALFVTG--ARSATEDAETG 231 VAIPA GYN + RGN+T KL +F LH +VTG R A E+G Sbjct: 182 VAIPAVLGYNYVARGNRTVAQKLMRFSHQLHVYYVTGNKMREAQAAKESG 231 >gi|300309940|ref|YP_003774032.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 197 bits (500), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 108/221 (48%), Positives = 138/221 (62%), Gaps = 6/221 (2%) Query: 7 GLESLWAQGDFVTKGVAVLLVAMSIASWYVIVTKALQLLRFRRASDAAGHAFWDTTSLPE 66 G WAQGD V+ VA LL+ MSIASWY I++KA R RR S+A FW +L + Sbjct: 6 GFAHYWAQGDAVSHSVAYLLLIMSIASWYYILSKAWAAWRIRRGSNAL-ERFWQAPTLAD 64 Query: 67 GVAT---LGNDNPFAEVAKAGVTSMSHHTAHKG--HLHDQLSVSDWVTLSLRQAIDEASG 121 A L ++ + +A+ + T +G L+ + +T +LR+ I+ S Sbjct: 65 ATAAMQRLDSEQIYTPMAERAAEAADIGTRQQGTPSLNAATDPGELITRTLRREINRVSA 124 Query: 122 KLQTGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTSGQASIDKVAGPVGEALIMTALG 181 +L+ G+ +LASVGSTAPFVGLFGTVWGIYHALV++ SG IDKVAGPVGEALIMTALG Sbjct: 125 RLERGLTLLASVGSTAPFVGLFGTVWGIYHALVAVSASGTVQIDKVAGPVGEALIMTALG 184 Query: 182 LAVAIPATFGYNALVRGNKTTIAKLNKFGFDLHALFVTGAR 222 L VAIPA YNA R N+ +A+L+ F DLHA TG R Sbjct: 185 LLVAIPAVLAYNAFTRINRVVLAELDGFAHDLHAYLTTGER 225 >gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel family protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 58/175 (33%), Positives = 80/175 (45%), Gaps = 11/175 (6%) Query: 30 SIASWYVIVTKALQLLRFRRASDAAGHAFWD--------TTSLPEGVATLGNDNPFAEVA 81 SI SW I K + R ++ FW +L G + Sbjct: 31 SIISWTYIFRKMFTIRAARVQTEEFERVFWSGGNLSALYQDALSNRRKAGGAGGALERIF 90 Query: 82 KAGV---TSMSHHTAHKGHLHDQLSVSDWVTLSLRQAIDEASGKLQTGMAVLASVGSTAP 138 +AG+ T A +G D + D ++R A L++ +A LASVGS +P Sbjct: 91 QAGMGEFTKAKASVAARGGAVDPSLLLDGARRAMRAAYQREMDALESHLAFLASVGSVSP 150 Query: 139 FVGLFGTVWGIYHALVSIGTSGQASIDKVAGPVGEALIMTALGLAVAIPATFGYN 193 +VGLFGTVWGI +A + QA++ VA + EALI TA+GL AIPA YN Sbjct: 151 YVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLFAAIPAVVAYN 205 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 752,713 Number of extensions: 30304 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 4 Length of query: 244 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 161 Effective length of database: 1,226,865 Effective search space: 197525265 Effective search space used: 197525265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)