BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0009.0058.p
         (764 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310040|ref|YP_003774132.1| organic solvent tolerance trans...  1103   0.0  

>gi|300310040|ref|YP_003774132.1| organic solvent tolerance
           transmembrane protein [Herbaspirillum seropedicae SmR1]
          Length = 766

 Score = 1103 bits (2854), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 550/773 (71%), Positives = 631/773 (81%), Gaps = 28/773 (3%)

Query: 1   MIRFFKFPFGRCADLRMSRFFTSLASLAVAASATTVLPVSAQAQTTQNTPATPSAKADDK 60
           MIRF K    R  + R++R F+S  +    ASA+ +LP+S  AQ T   PA  +A A D+
Sbjct: 1   MIRFSKSLTERTVNWRLARLFSSAMA---VASASALLPLSVYAQMT--LPAPVAAPAQDQ 55

Query: 61  DAPVNVSAEQMTGRPDRQINLDRDVEVVRGETTIKADKAEYRIIKDEVEATGHVWMKRLQ 120
           DAPVNVSAEQMTGRPDRQ+NLDRDVEVV+G TTIK+DKAEYRII+DEVEATGHVWM+RLQ
Sbjct: 56  DAPVNVSAEQMTGRPDRQVNLDRDVEVVKGPTTIKSDKAEYRIIQDEVEATGHVWMRRLQ 115

Query: 121 DRYTGDKALMNMDSGKGYVTNPTYLFGKNGGRGKAEKIDFLSDDQAKVTEGTYSTCEALD 180
           D+YTGD A MNM +G GYV++PTYL GKNGGRGKAE+IDF+SDDQA VT+GTYSTCEA D
Sbjct: 116 DQYTGDHAEMNMGTGAGYVSSPTYLIGKNGGRGKAERIDFISDDQALVTQGTYSTCEAPD 175

Query: 181 PDWYIQASTMDLDSGRDVGTMHSGVVYFKGVPILGAPLMSFPLSGERKSGVLPPTFGVTS 240
           PDWY++A+TMDLDSGRDVGT+  G++YFK VPIL AP MSF LSGERKSGVLPPT G TS
Sbjct: 176 PDWYLRANTMDLDSGRDVGTVRGGLLYFKDVPILAAPWMSFALSGERKSGVLPPTIGATS 235

Query: 241 TGGAELTVPYYFNIAPNRDLTLYPRYIARRGLQMGIEGRYLGEDYSGTTRVEGIQDRVTG 300
           TGG EL VPYYFN+APN DLTL P+YIARRGLQMG E RY+GE Y+G    EGIQDRVTG
Sbjct: 236 TGGFELAVPYYFNLAPNYDLTLVPKYIARRGLQMGAETRYMGESYNGVFNAEGIQDRVTG 295

Query: 301 TGRYSLSSLHSQTLAPNLVFGWNVNKASDNDYPSDFSHSVTASTQRLLTRDVSLAYSATY 360
           TGRYSLSS HSQ LAP L   WN+NKASDNDY SDFSHS+T ST RLL R  SL YS+TY
Sbjct: 296 TGRYSLSSRHSQILAPGLQLDWNLNKASDNDYASDFSHSITGSTTRLLPRTASLTYSSTY 355

Query: 361 WSAVGRVTKYQLLQDVNNPIQKPYDRVPQLTLTAARQDVGGFDFNFASEFARFSNTFTAG 420
           W+ VG+V+KYQLLQDVN PI KPYDRVPQ+T    R D+GGFDFN  ++F RFS+     
Sbjct: 356 WTLVGQVSKYQLLQDVNAPIVKPYDRVPQITFNGTRYDIGGFDFNTVADFTRFSS----- 410

Query: 421 TSAGSEMVGGDRMYANQSIAYPIIRPGYFITPKASLDATTYRLNNVSVGAPTNLTRVVPT 480
                 +VGGDR Y  QS++YPI+RPGYFITPK +LDAT+Y+LNN++ G  TNLTR VPT
Sbjct: 411 ----GALVGGDRAYLTQSVSYPIVRPGYFITPKVTLDATSYKLNNLAPGQQTNLTRTVPT 466

Query: 481 YSLDAGMTFERDTRLFGRDMTQTLEPRLFYVRTPYRDQSQYPNFDSGLADFNYAQIFSEN 540
            SLD+G+ FER++ L GR MTQTLEPRLFYVRTPYRDQ++ P FDSGLADFN+AQIFSEN
Sbjct: 467 LSLDSGLIFERESDLLGRSMTQTLEPRLFYVRTPYRDQTKLPIFDSGLADFNFAQIFSEN 526

Query: 541 RFVGHDRISDANQITGALVSRFIEEDGLERLRMAVGQRFYFADPKV--------VLDGTS 592
           RFVGHDRISDANQ+T A+ SR IEE+GLERLR  VGQR+YFADPKV            TS
Sbjct: 527 RFVGHDRISDANQLTAAVTSRLIEENGLERLRATVGQRYYFADPKVDLTSTSTTATTTTS 586

Query: 593 ATVNGSKSDLLLSLGGQITRQLSLDNTVQYSETLRQMVRSNFGARWQPAPKKVLNLTYRL 652
           +   GSKSDLLL+LGGQITR+ S DNTVQYSETLRQMVRSNFG RWQPAPK+VLNL YRL
Sbjct: 587 SGSLGSKSDLLLALGGQITREFSTDNTVQYSETLRQMVRSNFGIRWQPAPKRVLNLQYRL 646

Query: 653 DRTNA---TGI---LKQMDISGQWPVSQRWYAVSRINYSIPDKTVAEGLLGMEYKADCWV 706
           DRT     TG+   LKQ+D+SGQWP+SQR YAV RINYSIPD+TVAEGLLG+EYKADCWV
Sbjct: 647 DRTYVNPYTGVLDPLKQVDVSGQWPLSQRIYAVGRINYSIPDRTVAEGLLGLEYKADCWV 706

Query: 707 FRLVAQRIPTSSSKASTSFFIQLELNGFSKIGSNPLDALSASIPGYQIINKPD 759
           FR+VAQRIPTSS+KAST FF QLELNGFS+IGSNP+DA+ +S+PGYQ +N PD
Sbjct: 707 FRVVAQRIPTSSTKASTGFFFQLELNGFSQIGSNPMDAIKSSVPGYQKVNLPD 759


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 2,231,190
Number of extensions: 93675
Number of successful extensions: 196
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 191
Number of HSP's successfully gapped: 1
Length of query: 764
Length of database: 1,619,870
Length adjustment: 93
Effective length of query: 671
Effective length of database: 1,179,515
Effective search space: 791454565
Effective search space used: 791454565
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 134 (56.2 bits)