BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0045.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 462 e-131 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 97 1e-21 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 80 1e-16 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 80 2e-16 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 462 bits (1190), Expect = e-131, Method: Compositional matrix adjust. Identities = 225/257 (87%), Positives = 242/257 (94%) Query: 1 LLEVLQDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPDVL 60 LLEVL DAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMP+VL Sbjct: 36 LLEVLHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVL 95 Query: 61 STSHSRFPAVEGERDKVVGVLLAKDLLRYYAEESFDVRDMLRPAVFIPESKRLNVLLRDF 120 +TSHSRFPAV+GERDKVVG+LLAKDLLRYYAEESFDVRDMLRPA+FIPESKRLNVLLRDF Sbjct: 96 ATSHSRFPAVDGERDKVVGILLAKDLLRYYAEESFDVRDMLRPAIFIPESKRLNVLLRDF 155 Query: 121 RANRNHMAIVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEEEDNILAIKDGAHGPRWR 180 RANRNHMA+VVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDE EDN+LA+KDG HG RWR Sbjct: 156 RANRNHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWR 215 Query: 181 IKGLTEIAQFNETLETAFSDEDADTIGGLVANHLGRVPRKGDEFDIAGMRFEVLRADARQ 240 +K LTEI QFNE + + FSD+DADTIGGLVANH+GRVPRKGDEFDI G+ FEVLRADARQ Sbjct: 216 VKALTEIEQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQ 275 Query: 241 AHVLLVEKLPDTPAAEE 257 HVLLV +LP PA++E Sbjct: 276 VHVLLVSQLPPAPASDE 292 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 57/222 (25%), Positives = 113/222 (50%), Gaps = 9/222 (4%) Query: 20 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPDVLSTSHSRFPAVEGERDKVVG 79 S++ +F + ++V D+M PRSQ++ ++++ P++ + + + H++ P EGE + +VG Sbjct: 194 SILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGEINHIVG 253 Query: 80 VLLAKDLLRYYAEESF----DVRDMLRPAVFIPESKRLNVLLRDFRANRNHMAIVVDEYG 135 +L + + + E D+R++L ++P + L+ F+ N + +VVDEYG Sbjct: 254 ILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGVVVDEYG 313 Query: 136 GVAGLITIEDVLEQIVGDIEDEYDFDEEEDNILAIKDGAHGPRWRIKGLTEIAQFNETLE 195 V GL+T+ED++E+++G+ E KDGA ++G T + N+ L Sbjct: 314 EVQGLVTLEDIIEEMIGEFTTSSPGSSGE-GFGWDKDGA----CLLEGSTTLRDINKRLG 368 Query: 196 TAFSDEDADTIGGLVANHLGRVPRKGDEFDIAGMRFEVLRAD 237 + TI GL+ +L +P + G E+++ Sbjct: 369 LQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQ 410 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 67/237 (28%), Positives = 114/237 (48%), Gaps = 18/237 (7%) Query: 21 MIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPDVLSTSHSRFPAVEGERDKVVGV 80 +IE VF++ V M PR + ++ T ++ HSRF +G D V+G Sbjct: 202 LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSRFLVCDGHIDAVLGY 261 Query: 81 LLAKDLL-RYYAEESFDVRD--MLRPAVFIPESKRLNVLLRDFRANRNHMAIVVDEYGGV 137 + +KD+L R E F + D ++ P + IP+S L +L +A +AIVV+EY V Sbjct: 262 IDSKDVLKRLLKGEQFSLADKGLVHPPLSIPDSLNLWEVLERMKAMDEDLAIVVNEYALV 321 Query: 138 AGLITIEDVLEQIVGDIEDEYDFDEEEDNILAIKDGAHGPRWRIKGLTEIAQFNETLETA 197 G+I+ DV ++G++ EEE I+ + + W + GLT + N+ + Sbjct: 322 VGIISTTDVTGVLMGNL---LAITEEEQQIVRRDENS----WLMDGLTPL---NDVMSEL 371 Query: 198 FSDEDADTIG-----GLVANHLGRVPRKGDEFDIAGMRFEVLRADARQAHVLLVEKL 249 DE + +G G + L ++P+K D A +FEV+ + + + LLV + Sbjct: 372 DIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRINQLLVTRF 428 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 66/251 (26%), Positives = 120/251 (47%), Gaps = 25/251 (9%) Query: 17 DALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPDVLSTSHSRFPAVEGERDK 76 D +++ ++L V DIM P S++ + K +E + + +SR+P + ER + Sbjct: 205 DEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYRNRYSRYPYYDAERQQ 264 Query: 77 VVGVLLAKDLLRYYAEES----FDVRDMLRPAVFIPESKRLNVLLRDFRANRNHMAIVVD 132 V+G++ KD+ + A++ +++D LRP +I + LLR FR H A++ Sbjct: 265 VLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPALPALDLLRRFRTGSPHFAVIGK 322 Query: 133 EYGGVAGLITIEDVLEQIVGDIEDEY-----DFDEEEDNILAIKDGAHGPRWRIKGLTEI 187 + AG IT++++L +VG+I DE+ ++ ++D L KG I Sbjct: 323 KGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDDGTLL-----------GKGSLPI 371 Query: 188 AQFNETLETAFSDEDA-DTIGGLVANHLGRVPRKGDEFDIAGMRFEVLRADARQAHVLLV 246 L +D+ D++GGLV LG +P++G + D F+V+ ++LV Sbjct: 372 VTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPA--FDVVIKRMSGPKIVLV 429 Query: 247 EKLPDTPAAEE 257 + P A E Sbjct: 430 KVYPKLQEARE 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,960 Number of extensions: 28893 Number of successful extensions: 82 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 4 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)