BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0008.0021.p
         (446 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310451|ref|YP_003774543.1| 2-keto-4-pentenoate hydratase/2...   672   0.0  

>gi|300310451|ref|YP_003774543.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 448

 Score =  672 bits (1735), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 326/444 (73%), Positives = 365/444 (82%), Gaps = 2/444 (0%)

Query: 2   SITLNETHDAGLRSWVVSANAAGNDFPIQNLPYGVFRRKGRGSEEVFRAGVAIGDSILDL 61
           S+TLNETHD  LRSWV SANA G  FPIQNLPYGVFRR G  SEE FR GVAIGD ILDL
Sbjct: 5   SLTLNETHDTELRSWVPSANADGCPFPIQNLPYGVFRRAG--SEEDFRVGVAIGDQILDL 62

Query: 62  RAAFESGAFAGFGETVHKAIVAATAGSNLNPLMELDAAAWSALRLALSTLLREASVHQSQ 121
            AA  +GAF+GF E   +A  AA +GS LN LM L  A+WSALRLALS LLR AS HQ++
Sbjct: 63  PAAQSAGAFSGFDEATRQAAHAACSGSRLNALMALTPASWSALRLALSHLLRTASPHQTE 122

Query: 122 LEAALVPQTQAEHALPAQIGDYTDFYTSIHHATAIGKLFRPDNPLLPNYKWVPIGYHGRS 181
           L A LVPQ QAE+ +PA IGDYTDFYTSIHHAT +GKLFRPDNPLLPNYKWVPIGYHGR 
Sbjct: 123 LAACLVPQAQAEYDVPAAIGDYTDFYTSIHHATTVGKLFRPDNPLLPNYKWVPIGYHGRV 182

Query: 182 SSIAVSGQQFRRPLGQLKPPTSDTPVFGACKRLDYEMEVGVFMGHGNAIGDAIGIGAAES 241
           SSIAVSGQQFRRPLGQ K P +D P FG CKRLDYE+E+G+F+G GNA+G+ + +  AE 
Sbjct: 183 SSIAVSGQQFRRPLGQTKAPDADAPTFGPCKRLDYELEIGIFIGGGNAMGEPVPMEEAED 242

Query: 242 HVFGLCLLNDWSARDVQAWEYQPLGPFLAKNFASTISPWIVTLEALAPYRAAWTRDAADP 301
            VFGLCLLNDWSARDVQAWEYQPLGPFL+K+FASTISPWIVTLEALAPYR +W+R   DP
Sbjct: 243 KVFGLCLLNDWSARDVQAWEYQPLGPFLSKSFASTISPWIVTLEALAPYRCSWSRPDGDP 302

Query: 302 QPLAYLDAPDLREHGAFDIQLEVLLQTKAMRDAGTAPQRLSLGNFRDAYWTVSQLVAHHT 361
           QPL YLDAP LR  GAFD+QLEVL+QT AMR AG  PQRLSL N+RDAYWTV+QLV HHT
Sbjct: 303 QPLPYLDAPTLRTQGAFDVQLEVLIQTAAMRQAGQPPQRLSLSNYRDAYWTVAQLVTHHT 362

Query: 362 VGGCNLRAGDLLGSGTLSGPTPESAGSLLELTAGGKQSISLDNGETRIFIEDGDTVVMRG 421
           V GCNLR GD LGSGTLSGP P+ AGSLLELT GGK  ++L +GE R+F+EDGDT+++R 
Sbjct: 363 VNGCNLRPGDFLGSGTLSGPAPDEAGSLLELTTGGKSPLTLASGEKRVFLEDGDTIILRA 422

Query: 422 WAGREGLPRIGFGEVAGTVLPARA 445
            A R+GLP IGFGE AGTVLPAR+
Sbjct: 423 VARRDGLPLIGFGESAGTVLPARS 446


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,407,016
Number of extensions: 58615
Number of successful extensions: 163
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 163
Number of HSP's successfully gapped: 1
Length of query: 446
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 358
Effective length of database: 1,203,190
Effective search space: 430742020
Effective search space used: 430742020
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 132 (55.5 bits)