BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0019.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 671 0.0 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 245 5e-66 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 204 8e-54 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 110 2e-25 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 93 3e-20 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 671 bits (1731), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/428 (80%), Positives = 386/428 (90%) Query: 6 INVDQVVEQSRFGPFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVFG 65 ++VDQVVEQ RFG FQFGLLLLCGLCLIIDGFD+QAMGYVAPAII DW V+KASLGPVFG Sbjct: 12 VHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVFG 71 Query: 66 AGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAVCMLLTTRVQTIDELLILRFITGFGL 125 AGLFGMLLGSLVLTPVGD+YGRRPVLI+ST FFA+CML T V T+D+LL+LRFITGFGL Sbjct: 72 AGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFGL 131 Query: 126 GSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGGL 185 GSIMPNAMALVGEFSP+SSRVTRMMLVSCGFTVGAA GGFVSAALIPA+GW +VF VGG Sbjct: 132 GSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWVGGA 191 Query: 186 IPAALGVAMLLWLPESIQFLVLRKRPMKQVARWLNKVQPGLQVTESSTVVVKEARNDGMP 245 +P LG+AML+WLPESIQFLVL +RP QVARWL K+ P + + + + VVVKE + +GMP Sbjct: 192 VPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAEGMP 251 Query: 246 VAELFRDGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQVGGI 305 VA LFRDGRAGVT+LLWLISFMNLINLYFLSNWLPTLI+DAGYST AVLIGTSLQVGG+ Sbjct: 252 VAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQVGGV 311 Query: 306 VGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVVVAGFCVVGGQPA 365 +GTL+LG FI RFGFTRVLGSCF++AC+ IALIG +A P LLF+ V+VAGFC+VGGQPA Sbjct: 312 IGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAGFCIVGGQPA 371 Query: 366 VNALAGTFYPTQLRSTGIGWALGIGRVGSVLGPVIGGQLIAMQWANGSLFMAAAVPAIVS 425 VNALAGTFYPT LRSTGIGWALGIGR+GSV+GPVIGGQLIA+QW N SLF+AAAVPA++S Sbjct: 372 VNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVPALIS 431 Query: 426 ALMVARLH 433 AL +ARLH Sbjct: 432 ALTIARLH 439 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 245 bits (626), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 147/422 (34%), Positives = 246/422 (58%), Gaps = 3/422 (0%) Query: 6 INVDQVVEQSRFGPFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVFG 65 ++V Q+++ +RF F + ++ LC L L+ DG+D+ G V P ++ +W ++ G + Sbjct: 4 LDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGALGS 63 Query: 66 AGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAVCMLLTTRVQTIDELLILRFITGFGL 125 LFGM+ G+ + P+ D+ GR+ + IS F+V L+ + E + RF+ G G Sbjct: 64 YALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLGC 123 Query: 126 GSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGGL 185 G +MPNA+AL+ E++P R T + ++ G+++G + + ++P FGW+S+F Sbjct: 124 GGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFFAAA- 182 Query: 186 IPAALGVAMLLWLPESIQFLVLRKRPMKQVARWLNKVQPGLQVTESSTVVVKEARNDGMP 245 +P L +L LPES+ FL LR+ ++Q L +V P ++ + +V + + G P Sbjct: 183 VPLVLLPVILWKLPESVGFL-LRQGRLEQARAMLTQVDPQARIATDTQLVQADVKGAGAP 241 Query: 246 VAELFRDGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQVGGI 305 + LF+ RA TL++W+ F L+ +Y L +WLP L+ AGYS G+++ +L GG+ Sbjct: 242 MLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGGV 301 Query: 306 VGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVVVAGFCVVGGQPA 365 VG + GW +RF +V+ S F V V IAL+G + TPV L++ ++VAG +G Q Sbjct: 302 VGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPV-LYLLIIVAGATTIGTQIL 360 Query: 366 VNALAGTFYPTQLRSTGIGWALGIGRVGSVLGPVIGGQLIAMQWANGSLFMAAAVPAIVS 425 + A FYP +RSTG+GWA G+GR G+++GP++GG L+A F+ A+P +V+ Sbjct: 361 LYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPGLVA 420 Query: 426 AL 427 AL Sbjct: 421 AL 422 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 204 bits (520), Expect = 8e-54, Method: Compositional matrix adjust. Identities = 135/436 (30%), Positives = 216/436 (49%), Gaps = 5/436 (1%) Query: 5 VINVDQVVEQSRFGPFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVF 64 +++ ++++ F FQ ++ L L +++DGFD Q +G+ P++I +W + + + P Sbjct: 11 TVDIGRMLDDGVFSGFQKLVVCLAALSIVMDGFDGQLIGFAIPSLIKEWGITRNAFAPAV 70 Query: 65 GAGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAVCMLLTTRVQTIDELLILRFITGFG 124 AGL GM +GS D++GRR +I S F F V L + + ILRF G G Sbjct: 71 AAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGLG 130 Query: 125 LGSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGG 184 +G +P++ + EFSP R + +G + G + A++P +GWR +FL+GG Sbjct: 131 IGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIGG 190 Query: 185 LIPAALGVAMLLWLPESIQFLVLRKRPMKQVARWLNKVQPGLQVTESSTVVVKEARNDGM 244 L+P A V + +PES ++L ++ R L ++ + ++A Sbjct: 191 LLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADAREQAIETRA 250 Query: 245 PVAELFRDGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTSLQVGG 304 LF G+A T+ LW+ FMNL+ +Y +WLPT++ G S A T+ GG Sbjct: 251 GFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTAYNFGG 310 Query: 305 IVGTLTLGWFINRFGFTRVLGSCFVVA---CVFIALIGHVAATPVLLFICVVVAGFCVVG 361 + G L FI RFG + C + A +F+ + VA+ LL + + + G V Sbjct: 311 VFGALLCALFITRFGSRLPMLLCCLAATASALFLRSL-DVASQTTLLVVGIGLHGLFVNA 369 Query: 362 GQPAVNALAGTFYPTQLRSTGIGWALGIGRVGSVLGPVIGGQLIAMQWANGSLFM-AAAV 420 Q + AL YPTQ+R+TG AL GR+G++L G +I A G L M A A+ Sbjct: 370 VQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAFAGALVITQGGAAGYLAMLAGAM 429 Query: 421 PAIVSALMVARLHWYR 436 + AL+V R H R Sbjct: 430 LMVFVALLVVRKHIPR 445 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 97/394 (24%), Positives = 176/394 (44%), Gaps = 25/394 (6%) Query: 12 VEQSRFGPFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHVNKASLGPVFGAGLFGM 71 +E F + ++ G + D F ++ +V P ++G W ++ A +G + +G G Sbjct: 33 IENVPFSRWHTKARIIMGSATLFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQ 92 Query: 72 LLGSLVLTPVGDKYGRRPVLIISTFFFAVCMLLTTRVQTIDELLILRFITGFGLGSIMPN 131 ++G+L + ++YGR P ++ +V ++ + L + RF+ G G+G +P Sbjct: 93 VIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPV 152 Query: 132 AMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIPAFGWRSVFLVGGLIPAALG 191 A + E S R +L F +G V A L+P FGW +FLVGG IP L Sbjct: 153 AATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLVGG-IPGILV 211 Query: 192 VAMLLWLPESIQFLVLRKR--PMKQVARWLNKVQPGLQVTESSTVVVKEARNDGMPV--- 246 ++ LPES ++L+ R R +++ R L P + + E R + + Sbjct: 212 ALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAG 271 Query: 247 -------AELFRDGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAAVLIGTS 299 ELF G T+++WL+ + ++NWLP+L Y T + + S Sbjct: 272 KRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSL-----YKTVYHLPLQES 326 Query: 300 LQVGGIVGTLT------LGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVV 353 L++ + L+ I+R G R + F+++ +AL+G + A + + Sbjct: 327 LRMASVSNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLA 386 Query: 354 VAGFCVVGGQPAVNAL-AGTFYPTQLRSTGIGWA 386 + + V+G + L YPT++R+ G G A Sbjct: 387 SSAYAVMGTTTVLLYLYTPEIYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 103/416 (24%), Positives = 189/416 (45%), Gaps = 16/416 (3%) Query: 1 MQSAVINVDQVVEQSRFGPFQFGLLLLCGLCLIIDGFDIQAMGYVAPAIIGDWHV---NK 57 M + +V+Q ++ + G FQ+ L ++ GL + D + ++G+ AP I + + Sbjct: 1 MSTLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQA 60 Query: 58 ASLGPVFGAGLFGMLLGSLVLTPVGDKYGRRPVLIISTFFFAVCMLLTTRVQTIDELLIL 117 G +F GMLLG+ V + D+ GRRPVL+ + A + + LL L Sbjct: 61 LQTGTMF---FVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLAL 117 Query: 118 RFITGFGLGSIMPNAMALVGEFSPNSSRVTRMMLVSCGFTVGAAVGGFVSAALIP--AFG 175 R +TG G+G +P ++ EF P++ R ++++ + +G ++ + Sbjct: 118 RLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVSWGNDA 177 Query: 176 WRSVFLVGGLIPAALGVAMLLWLPESIQFLVLRKRPMKQVARWLNKVQPGLQVTESSTVV 235 WR +F V GL PA +GV + ++PES +L R + L +V +V SST + Sbjct: 178 WRVIFFVTGL-PALVGVVLRFYVPESPMYLN-RSGKSDAARQVLERVA---KVNGSSTPI 232 Query: 236 --VKEARNDGMPVAELFRDGRAGVTLLLWLISFMNLINLYFLSNWLPTLIKDAGYSTGAA 293 ++ + P++ LF +L L+L + I Y + +LP + G+ Sbjct: 233 PPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRG 292 Query: 294 VLIGTSLQVGGIVGTLTLGWFINRFGFTRVLGSCFVVACVFIALIGHVAATPVLLFICVV 353 + L + + G + + R+G L V++ V L + P+L+ + Sbjct: 293 QIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCMLY-SLGKDPMLVVGSTL 351 Query: 354 VAGFCVVGGQPAVNALAGTFYPTQLRSTGIGWALGIGRVGSVLGPVIGGQLIAMQW 409 + F ++G A+ A YPT LR++G+G A + R G + P I ++A + Sbjct: 352 LLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASHF 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.144 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,327,407 Number of extensions: 55095 Number of successful extensions: 471 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 457 Number of HSP's successfully gapped: 23 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)