BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0008.0017.p
         (284 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum serope...   235   3e-63
gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedi...   166   2e-42
gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa...    78   7e-16

>gi|300310447|ref|YP_003774539.1| esterase [Herbaspirillum
           seropedicae SmR1]
          Length = 277

 Score =  235 bits (599), Expect = 3e-63,   Method: Compositional matrix adjust.
 Identities = 136/285 (47%), Positives = 172/285 (60%), Gaps = 11/285 (3%)

Query: 1   MKHYLHYDRDQLDRQYNVRAGIPRFQDIFDSWNETARAYRQQHEVRTNLHYGTHRLQALD 60
           MK Y  Y  ++LD QYNVRA +P FQD FD +     A RQ H    +L YG   LQ++D
Sbjct: 1   MKIYRDYTSEELDAQYNVRARVPDFQDYFDRFARQGEAARQAHPHLADLPYGEETLQSID 60

Query: 61  YFPAAQADAPLLVFIHGGYWRSLDKDDFSHLAGPCHEAGIAVAMINYRLAPEVDMSEIVG 120
           +FPA     PLLVFIHGGYWRSLDK  FSHLA P  E  I VA+INYRLAP+V M +I  
Sbjct: 61  FFPAHSNGRPLLVFIHGGYWRSLDKHQFSHLALPYLEQDINVALINYRLAPQVRMGDIAA 120

Query: 121 DVRLAVSWLYRQAAQLGFNADDIYVAGHSAGGHLAAMVAATEWRDAGLPDNVVKGLCGVS 180
           D   A+  L+ +A  L  +A+ I++ GHSAG HLA ++AA       L    V+G+C +S
Sbjct: 121 DCGRALRLLHAKAPALRVDANAIWLMGHSAGAHLACVIAA-------LRAAPVRGVCALS 173

Query: 181 GLYDLEPIRLCYLNEALQLDQLQVRQFSPLHHLPRLP--IPVVLTAGGAESTEFHRQLAA 238
           GLYDLEPIRL YLNEAL L    V+Q SPL  L  +P  +  VL AGG ES EF RQ   
Sbjct: 174 GLYDLEPIRLSYLNEALHLSPADVQQASPL--LLAMPDGVRAVLCAGGQESEEFLRQRDV 231

Query: 239 YGLALQAAGFPVRQVRLADGHHLDVISKLADKDNELAQALVALIR 283
           Y   L+ +G  V  V  A  HHL +I   AD  +   + ++ +++
Sbjct: 232 YAANLRQSGHQVEVVEAAQAHHLSIIDVAADGQSAFTRTVLRMMQ 276


>gi|300312950|ref|YP_003777042.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 289

 Score =  166 bits (421), Expect = 2e-42,   Method: Compositional matrix adjust.
 Identities = 100/267 (37%), Positives = 148/267 (55%), Gaps = 13/267 (4%)

Query: 16  YNVRAGIPRFQDIFDSWNETARAYRQQHEVRTNLHYGTHRLQALDYFPAAQADAPLLVFI 75
           YN++A  P F  +   W +   A        +++ YG   LQ  D++ A  A  PLLVFI
Sbjct: 26  YNLKAAFPDFPQVLQQWQQATAAIADTLLTESDISYGDAPLQRFDFYRAEGAQRPLLVFI 85

Query: 76  HGGYWRSLDKDDFSHLAGPCHEAGIAVAMINYRLAPEVDMSEIVGDVRLAVSWLYRQAAQ 135
           HGGYW+  DK D   +A P  +AGI+VA+INY LAP+  + ++V +V+  +S + +QA +
Sbjct: 86  HGGYWQGGDKRDIGFIAAPYVKAGISVAVINYSLAPQARIEDMVKEVQACLSTIAQQAER 145

Query: 136 LGFNADDIYVAGHSAGGHLAAMVAATEWRDAGLPDNVVKGLCGVSGLYDLEPIRLCYLNE 195
           LG + D I + GHSAGGHLAA VAA   R   +P   V+ +  +SG++DL P+    LN+
Sbjct: 146 LGIDVDRISLMGHSAGGHLAAFVAAQPGR---MP---VQAVFAISGVFDLAPLIPTSLNK 199

Query: 196 ALQLDQLQVRQFSPLHHLPRLPIPVVLTAGGAESTEFHRQLAAYGLALQAAGFP--VRQV 253
           AL LDQ++    SP+         V    G  E+ +FH Q      A+ A  +P  V   
Sbjct: 200 ALTLDQVRADALSPVLQTGPAQTRVHTIIGELETLQFHLQ-----AAVMANCWPQVVAHH 254

Query: 254 RLADGHHLDVISKLADKDNELAQALVA 280
            + D HH  V+  LAD D+ + +A++A
Sbjct: 255 VVPDTHHYTVLFPLADADSSVCRAVIA 281


>gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase
           family protein [Herbaspirillum seropedicae SmR1]
          Length = 302

 Score = 78.2 bits (191), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 65/231 (28%), Positives = 98/231 (42%), Gaps = 10/231 (4%)

Query: 57  QALD-YFPAAQADAP-----LLVFIHGGYWRSLDKDDFSHLAGPCHEAGIAVAMINYRLA 110
           Q LD Y PA  A AP     ++VF +GG W    + D++ +       G+   + +YRL 
Sbjct: 55  QQLDIYRPADTASAPPQGRPVVVFFYGGSWNQGSRKDYAFVGEALASRGVIAVLPDYRLY 114

Query: 111 PEVDMSEIVGDVRLAVSWLYRQAAQLGFNADDIYVAGHSAGGHLAAMVAA-TEWRD-AGL 168
           PEV   + + D   AV+W  R    LG +   ++V GHSAG + AAM+A    W D AG 
Sbjct: 115 PEVSYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWLDAAGA 174

Query: 169 PDNVVKGLCGVSGLYDLEPIRLCYLNEALQLDQLQVRQFSPLHHLPRLPIPVVLTAGGAE 228
               ++G  G++G YD  PI    +               P+ H+     P +L A   +
Sbjct: 175 TPAQLRGWIGLAGPYDFLPIENPDVKPVFHFPD-SPPDSQPVRHVQASAPPALLIASHQD 233

Query: 229 S-TEFHRQLAAYGLALQAAGFPVRQVRLADGHHLDVISKLADKDNELAQAL 278
                 R        L+  G PV +    +  H  ++  LA     +A  L
Sbjct: 234 KLVSATRNTGGLAALLRQHGVPVVETYFDNTSHASLVGSLARPLRFIAPTL 284


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.138    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 924,792
Number of extensions: 38416
Number of successful extensions: 124
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 119
Number of HSP's successfully gapped: 3
Length of query: 284
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 200
Effective length of database: 1,222,130
Effective search space: 244426000
Effective search space used: 244426000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)