BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0010.p (448 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 329 4e-91 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 322 4e-89 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 306 2e-84 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 281 6e-77 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 274 1e-74 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 329 bits (843), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 180/423 (42%), Positives = 264/423 (62%), Gaps = 11/423 (2%) Query: 9 EKSAIRKVSLRLVPFVALMFFINFLDRTAISFAGPNGMTKDLALTAAQFGFAAGVFFIGY 68 E + KV+ RL+P + L + ++LDR + FA M +DL + +G AG+FF+GY Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQ-MLQDLKFSDTVYGLGAGIFFLGY 75 Query: 69 VLLEIPSNLALHKYGARRWLARIMVTWGIVALLFTWVSNVEGLYVLRFLLGVAEAGFFPG 128 + E+PSN+ LHK GAR W+ARIM+TWGI++ +V+ E Y++RFLLGVAEAGFFPG Sbjct: 76 FVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPG 135 Query: 129 AILFLSMWVPARHRSHILALFYLAQPLTIVIGAPVAALLIEAH-GLFGLEGWRVMFAGVA 187 IL+L+ W PA R I ALF L+ VIG P++ ++ A G++G GW+ MF A Sbjct: 136 VILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEA 195 Query: 188 IPAIVVGVIAWFYLADKPADAKWLNEAERNWLTAALASENKTKRITAQSNVTSALTNSRV 247 IP++++GV+ Y+ D+ DA WL+E E+ L A ++ E K ++ + N RV Sbjct: 196 IPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE---HLSLGAMFANGRV 252 Query: 248 WLLSLVYFGLIYGLYALAFFLPTIIGGFEAQFGSKFNVFQKGLITAIPYLPAAVALYLWS 307 WL +L+YF + GLY ++F+LPTII NV GL+TAIPY AA A+ L Sbjct: 253 WLGALIYFCFVMGLYGVSFWLPTIIKA--TGVSDPLNV---GLLTAIPYAFAAAAMVLIG 307 Query: 308 RDATHRGVRSWHVGVPALLGAVSIPMAL-YMNTPAATVAAITVTACAIFSALPTFWSIPG 366 R A R R WHV +PA +G + + ++ Y + +A++T+ + I + LP FWS+P Sbjct: 308 RSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPT 367 Query: 367 RFLSGAAAAAGIALINTVGNAAGFIAPFVTGAIKDATGSYQAPMFVVGALMLMSAVVIFA 426 FLSG AAAAGIALIN++GN AGF++PF+ G +KDAT S A M+V+ A +++ ++ Sbjct: 368 AFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLT 427 Query: 427 IGA 429 + A Sbjct: 428 LPA 430 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 322 bits (825), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 174/428 (40%), Positives = 262/428 (61%), Gaps = 7/428 (1%) Query: 9 EKSAIRKVSLRLVPFVALMFFINFLDRTAISFAGPNGMTKDLALTAAQFGFAAGVFFIGY 68 E + ++KV RL+PF+ L + +++LDR + FA M KDL LT +QFG AG+FFIGY Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALT-MNKDLGLTPSQFGLGAGLFFIGY 78 Query: 69 VLLEIPSNLALHKYGARRWLARIMVTWGIVALLFTWVSNVEGLYVLRFLLGVAEAGFFPG 128 EIPSNLALH++GAR W++RIM++WG++++ +V + + RFLLG+AEAGF PG Sbjct: 79 FFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPG 138 Query: 129 AILFLSMWVPARHRSHILALFYLAQPLTIVIGAPVAALLIEAHGLFGLEGWRVMFAGVAI 188 L+ + W P R A F + P+ +IG+P++ L+E HG++G +GW+V+ A+ Sbjct: 139 IYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEAL 198 Query: 189 PAIVVGVIAWFYLADKPADAKWLNEAERNWLTAALASENKTKRITAQSNVTSALTNSRVW 248 PA+++GV+ F L D+PA AKWL+ E+ WL L++E + + A TN RV+ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 249 LLSLVYFGLIYGLYALAFFLPTIIGGFEAQFGSKFNVFQKGLITAIPYLPAAVALYLWSR 308 L+ F I G ++ +LP II +FG + Q GL+ AIPYL AVA+ LW+R Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQII----REFGLPSH--QVGLVAAIPYLVGAVAMTLWAR 312 Query: 309 DATHRGVRSWHVGVPALLGAVSIPMALYMNTPAATVAAITVTACAIFSALPTFWSIPGRF 368 A R + V +L A+S+ ++ +++TP + AITV +I S TFW+IP F Sbjct: 313 LANRSERRLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGF 372 Query: 369 LSGAAAAAGIALINTVGNAAGFIAPFVTGAIKDATGSYQAPMFVVGALMLMSAVVIFAIG 428 L+G AAA G+ALI ++GN GF+ P V G I++AT + P+ V +L+ AV+ A+G Sbjct: 373 LTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALG 432 Query: 429 AGARQEQT 436 +R T Sbjct: 433 DPSRVPPT 440 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 306 bits (784), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 168/419 (40%), Positives = 265/419 (63%), Gaps = 10/419 (2%) Query: 14 RKVSLRLVPFVALMFFINFLDRTAISFAGPNGMTKDLALTAAQFGFAAGVFFIGYVLLEI 73 +KV R++PF+ L + I +LDR + FA M++DL + FG AG+FFIGY L E+ Sbjct: 23 KKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQ-MSQDLGFSETVFGLGAGLFFIGYFLFEV 81 Query: 74 PSNLALHKYGARRWLARIMVTWGIVALLFTWVSNVEGLYVLRFLLGVAEAGFFPGAILFL 133 PSN+ +HK GAR W+ARIM+TWGI++ F +V N Y+LRFLLG+AEAGF+PG IL+L Sbjct: 82 PSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYL 141 Query: 134 SMWVPARHRSHILALFYLAQPLTIVIGAPVAALLIEA-HGLFGLEGWRVMFAGVAIPAIV 192 + W P+ R+ ++A+F P+ ++G P++ +++A H GLEGW+ MF AIPA++ Sbjct: 142 TYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVL 201 Query: 193 VGVIAWFYLADKPADAKWLNEAERNWLTAALASENKTKRITAQSNVTSALTNSRVWLLSL 252 +GV YL + AKWLN+ E+ L A + + K K ++ + + ++RVWL+ L Sbjct: 202 IGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKE--SKHGFGAIVKDARVWLMCL 259 Query: 253 VYFGLIYGLYALAFFLPTIIGGFEAQFGSKFNVFQKGLITAIPYLPAAVALYLWSRDATH 312 +YF + G Y L ++PT++ G K N+ + GL++AIP+ A +A+ L R A Sbjct: 260 IYFSFVMGQYGLTLWMPTLV----KATGVKGNL-EIGLLSAIPFGCAIIAMNLIGRSADR 314 Query: 313 RGVRSWHVGVPALLGAVS-IPMALYMNTPAATVAAITVTACAIFSALPTFWSIPGRFLSG 371 R WH+ +PAL+G V + AL+ + A ++A++++ A + + P FWS+P FLSG Sbjct: 315 MRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSG 374 Query: 372 AAAAAGIALINTVGNAAGFIAPFVTGAIKDATGSYQAPMFVVGALMLMSAVVIFAIGAG 430 AAAA GIA IN+VGN AGF++P++ G +KD T + M+++ ++++ ++ A G Sbjct: 375 AAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPG 433 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 281 bits (720), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 154/426 (36%), Positives = 241/426 (56%), Gaps = 8/426 (1%) Query: 8 VEKSAIRKVSLRLVPFVALMFFINFLDRTAISFAGPNGMTKDLALTAAQFGFAAGVFFIG 67 +E+ +RKV+ RL+PF+ L + F+DR I A M DL L+ FGFA+ +FFI Sbjct: 10 LEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQ-MNHDLGLSPKMFGFASSLFFIA 68 Query: 68 YVLLEIPSNLALHKYGARRWLARIMVTWGIVALLFTWVSNVEGLYVLRFLLGVAEAGFFP 127 Y + E+PSNLAL K+GAR+WLARIM+TWG+++ V LY +RF+LG AEAGFFP Sbjct: 69 YFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFFP 128 Query: 128 GAILFLSMWVPARHRSHILALFYLAQPLTIVIGAPVAALLIEAHGLFGLEGWRVMFAGVA 187 G +L+L+ WVP+ +R+ I+ALF +A P + +PV+ALL++ G+ GL GW +F Sbjct: 129 GVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLEG 188 Query: 188 IPAIVVGVIAWFYLADKPADAKWLNEAERNWLTAALASENKTKRITAQSNVTSALTNSRV 247 +P +V+G++ +L D+P A+WL++ +R WLT + E ++ + + V Sbjct: 189 LPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHV 248 Query: 248 WLLSLVYFGLIYGLYALAFFLPTIIGGFEAQFGSKFNVFQKGLITAIPYLPAAVALYLWS 307 W ++LV L + P ++ F +V Q GL+ +IPYL A+V + W Sbjct: 249 WGMALVCACASAAGSVLNVWQPQLLKSF------GLSVMQTGLVNSIPYLIASVLMVWWG 302 Query: 308 RDATHRGVRSWHVGVPALLGAVSIPMALYMNTPAATVAAITVTACAIFSALPTFWSIPGR 367 R + G R WH +P +L + +A N+ A V +T+ +S FW++ Sbjct: 303 RHSDRTGERRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMASS 362 Query: 368 FLSGAAAAAGIALINTVGN-AAGFIAPFVTGAIKDATGSYQAPMFVVGALMLMSAVVIFA 426 +L+ +AAAG+A IN V N G + V G +KDATGSY + + L ++S ++ Sbjct: 363 WLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLF 422 Query: 427 IGAGAR 432 + +R Sbjct: 423 LSRKSR 428 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 171/419 (40%), Positives = 261/419 (62%), Gaps = 18/419 (4%) Query: 14 RKVSLRLVPFVALMFFINFLDRTAISFAGPNGMTKDLALTAAQFGFAAGVFFIGYVLLEI 73 RK++ RL+PF+ +F + ++DR + FA M +DL + A +G AG+FFIGY + E+ Sbjct: 26 RKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQ-MLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 74 PSNLALHKYGARRWLARIMVTWGIVALLFTWVSNVEGLYVLRFLLGVAEAGFFPGAILFL 133 PSNL L K GAR+ LARI + WG+ ++ +V+ Y+LRFLLG+ EAGFFPG +L+L Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYL 144 Query: 134 SMWVPARHRSHILALFYLAQPLTIVIGAPVAALLIEA-HGLFGLEGWRVMFAGVAIPAIV 192 + W PA+ R+ + LF + + +G P+A L++ G+ L W+ +F IP+I+ Sbjct: 145 TYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSIL 204 Query: 193 VG--VIAWFYLADKPADAKWLNEAERNWLTAALASENKTKRITAQSNVTSALTNSRVWLL 250 G V+AW L ++PA+AKWL+EAE+N +T LA+EN + A TN RVW+ Sbjct: 205 AGFAVLAW--LPERPANAKWLSEAEKNAVTQVLAAENSAP--GKHFSFKDACTNYRVWIC 260 Query: 251 SLVYFGLIYGLYALAFFLPTIIGGFEAQFGSKFNVFQKGLITAIPYLPAAVALYLWSRDA 310 + VYF ++ G +AF+ P+II + G K N+ Q GLI+AIP++ +A+ + Sbjct: 261 AAVYFCVVSGNATIAFWAPSII----KEIGVKGNL-QIGLISAIPFIAGTLAMIWNGFHS 315 Query: 311 THRGVRSWHVGVPALLGAVSIPMALYMNTPAATVA--AITVTACAIFSALPTFWSIPGRF 368 G R H + L+ + + +A M AT+A ++T+ A I +A P FWSIPG F Sbjct: 316 DKTGERRLHSAIATLIACIGL-VATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAF 374 Query: 369 LSGAAAAAGIALINTVGNAAGFIAPFVTGAIKDATGSYQAPMFVVGALMLMSA--VVIF 425 L+G AAA GIALIN++GN AGF+AP++ GA+K +TGS + ++ V AL L+++ VV+F Sbjct: 375 LAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVF 433 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,260,146 Number of extensions: 49966 Number of successful extensions: 346 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 20 Length of query: 448 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 360 Effective length of database: 1,203,190 Effective search space: 433148400 Effective search space used: 433148400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)