BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0007.p (275 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 115 3e-27 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 96 4e-21 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 68 7e-13 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 56 4e-09 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 55 8e-09 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 74/256 (28%), Positives = 123/256 (48%), Gaps = 6/256 (2%) Query: 12 LAALTGVGMLATDLYLPALPHLAHDLGSDVPAVQATISSFMIALALSQLLWGALADKLGM 71 LA LT +G LA D+YLP+ +A DLG VQ T+++F+I +A+ Q+ +G L+D+ G Sbjct: 20 LAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDRFGR 79 Query: 72 RNTLLAGVALLAVTGAACALATDVDALIVYRTVQGIGAGAATVVVPVLLRRRFADADAMR 131 + L G+AL V C A +++ LIV R QG+G A V+ ++R R + + Sbjct: 80 KPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSAK 139 Query: 132 AISWVSIAESVIPAIAPVIGTGILLASGWRSNFWLVAILALALLPFILKL------VPGK 185 A S + + + P +AP IG +L+ + WR F ++ + L + K V Sbjct: 140 AFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVDVSRA 199 Query: 186 QVLHGADETVSYAVLLRNRAYLRDAAVYGLTFGALVTFVASAPHLIEQWSSRGPGTFALM 245 + LH Y LLR+R ++ GL + ++A P ++ + P F + Sbjct: 200 EPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQYFGFV 259 Query: 246 QVCGVSSFIVAASRSA 261 I A+ +A Sbjct: 260 FASNAIGLIAASQVNA 275 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 95.5 bits (236), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 68/254 (26%), Positives = 117/254 (46%), Gaps = 23/254 (9%) Query: 5 PLLQIIPLAALTGVGMLATDLYLPALPHLAHDLGSDVPAVQATISSFMIALALSQLLWGA 64 P + + A G LA DLY+PALP +A D + A+Q++++ ++IA A QL++G Sbjct: 19 PRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGP 78 Query: 65 LADKLGMRNTLLAGVALLAVTGAACALATDVDALIVYRTVQGIGAGAATVVVPVLLRRRF 124 LAD G + L G+ L ++ A +++ + R +QG G A ++ ++ F Sbjct: 79 LADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSF 138 Query: 125 ADADAMRAISWVSIAESVIPAIAPVIGTGILLASGWRSNFWLVAILALALLPFILKLVPG 184 A + A ++++IA + P IAPVIG + A GWRS + + +L + Sbjct: 139 AGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMW--------- 189 Query: 185 KQVLHGADETVSYAVLLRNRAYLRDAAV-------------YGLTFGALVTFVASAPHLI 231 VL ET++ V L R+A + GL + L+ F P ++ Sbjct: 190 -AVLWRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMV 248 Query: 232 EQWSSRGPGTFALM 245 + + P F + Sbjct: 249 QNTLHQPPTFFGYL 262 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 68.2 bits (165), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 48/137 (35%), Positives = 68/137 (49%), Gaps = 3/137 (2%) Query: 30 LPHLAHDLGSDVPAVQATISSFMIALALSQLLWGALADKLGMRNTLLAGVALLAVTGAAC 89 LP +A DLG+ ++ +SS+++ A L GA AD+ G R LL G+AL A+T AC Sbjct: 59 LPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLAC 118 Query: 90 ALATDVDALIVYRTVQGIGAGAATVVVPVLLRRRFADADAMRAISWVSIAE--SVIPAIA 147 ALA AL R +QG+GA ++ F D D R +W + +A Sbjct: 119 ALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDED-QRERAWALWGGIMGLTMVLA 177 Query: 148 PVIGTGILLASGWRSNF 164 P+IG + GWR F Sbjct: 178 PLIGGVVNTLLGWRWAF 194 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 36/136 (26%), Positives = 69/136 (50%) Query: 29 ALPHLAHDLGSDVPAVQATISSFMIALALSQLLWGALADKLGMRNTLLAGVALLAVTGAA 88 +LP LA DL D ++ +++++ AL + + G +ADKLG R A + + Sbjct: 9 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 68 Query: 89 CALATDVDALIVYRTVQGIGAGAATVVVPVLLRRRFADADAMRAISWVSIAESVIPAIAP 148 CA++ + + R +QGIG V V++ R ++ ++A+S++++ + P + P Sbjct: 69 CAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 128 Query: 149 VIGTGILLASGWRSNF 164 ++G I WR F Sbjct: 129 LLGGFITTYFHWRWVF 144 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 54.7 bits (130), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 38/141 (26%), Positives = 70/141 (49%), Gaps = 3/141 (2%) Query: 29 ALPHLAHDLGSDVPAVQATISSFMIALALSQLLWGALADKLGMRNTLLAGVALLAVTGAA 88 ALP ++ L + +++++A A++ + AL+ ++G R AG+A+ + Sbjct: 51 ALPQISQALQTSAAVSIWVSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLG 110 Query: 89 CALATDVDALIVYRTVQGIGAGAATVVVPVLLRRRFADADAMRAISWVSIAESVIPAIAP 148 CAL+ V LI+ R +QG+G A + P + R F + R + ++ + A+ P Sbjct: 111 CALSGSVTLLILMRILQGLGGAAMLSIAPAIHRTIFPNRLLGRILGLNAVLVAASTAVGP 170 Query: 149 VIGTGILLASGWRSNFWLVAI 169 V+G +L GW W+ AI Sbjct: 171 VLGGTLLAMLGWE---WIFAI 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 677,719 Number of extensions: 22837 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 17 Length of query: 275 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 191 Effective length of database: 1,222,130 Effective search space: 233426830 Effective search space used: 233426830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)