BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0192.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 489 e-139 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 134 1e-32 gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 124 1e-29 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 101 1e-22 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 89 1e-18 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 489 bits (1260), Expect = e-139, Method: Compositional matrix adjust. Identities = 255/315 (80%), Positives = 274/315 (86%), Gaps = 10/315 (3%) Query: 122 YDVDRYKLLAFVLSAALTGLAGSTKALILGFETLTDVHWSGHAAFFGGAGYVAGQAIKAW 181 Y V K+L F L A L++G+ L GHAAF G AGY+ GQA+K W Sbjct: 22 YPVFLMKMLCFALFAC-------AFNLLIGYTGLLSF---GHAAFLGWAGYMCGQALKVW 71 Query: 182 GVPTEIGLILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDG 241 G+PTE+GLI+GTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDG Sbjct: 72 GLPTELGLIIGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDG 131 Query: 242 LQGIPRGKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAIS 301 LQG+PRGKLLGF++L SDMTLYYVVLAIA+AGFALIVRTIHSPFGQVLKAIKENEPRAIS Sbjct: 132 LQGVPRGKLLGFLNLESDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAIS 191 Query: 302 LGYDVDRYKLLAFVLSAALTGLAGSTKALILGFETLTDVHWSMSGLVVLMTLVGGLGTIV 361 LGYDV RYKLLAFVLSA L+GLAG+TK L+LGFETLTDVHWSMSGLVVLMTLVGGLGTI+ Sbjct: 192 LGYDVARYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTIL 251 Query: 362 GPIVGAVIIIMLENKLGDIGNWLASVTHIEWFNTLGDSVTIVTGFIFIVCVLAFRRGIVG 421 GPIVGAVIIIMLENKLGDIG WLASVTHI+WFNTLGDSVTIVTGFIFIVCVLAFR+GIVG Sbjct: 252 GPIVGAVIIIMLENKLGDIGAWLASVTHIDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVG 311 Query: 422 EILNLFNKRSASASH 436 EIL L K AS SH Sbjct: 312 EILALTRKGQASGSH 326 Score = 296 bits (759), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 147/161 (91%), Positives = 157/161 (97%) Query: 1 VPTEIGLILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGL 60 +PTE+GLI+GTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGL Sbjct: 73 LPTELGLIIGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGL 132 Query: 61 QGIPRGKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISL 120 QG+PRGKLLGF++L SDMTLYYVVLAIA+AGFALIVRTIHSPFGQVLKAIKENEPRAISL Sbjct: 133 QGVPRGKLLGFLNLESDMTLYYVVLAIAVAGFALIVRTIHSPFGQVLKAIKENEPRAISL 192 Query: 121 GYDVDRYKLLAFVLSAALTGLAGSTKALILGFETLTDVHWS 161 GYDV RYKLLAFVLSA L+GLAG+TK L+LGFETLTDVHWS Sbjct: 193 GYDVARYKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWS 233 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 134 bits (337), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 104/261 (39%), Positives = 152/261 (58%), Gaps = 22/261 (8%) Query: 162 GHAAFFGGAGYVAGQAIKAWGVPTEIGLI--LGTLTGALIGLVMGLLAIRRQGIYFTMIT 219 GHA FFG YV AI ++ T LI L L AL+GLV+G++A+R + +YF MIT Sbjct: 58 GHAVFFGLGAYVT--AIASFHGVTSAWLIWLLAALVTALVGLVIGVIALRTRAVYFIMIT 115 Query: 220 LALAQMLFFVALQAPFTGGEDGLQGIPRGKLLGF-IDLSSDMTLYYVVLAIAIAGFALIV 278 +ALAQ+ ++ + + GG+DGLQ R LLGF +D+S D Y+VVLA A F L+ Sbjct: 116 MALAQLFYYFFVGFRYYGGDDGLQVTAR-PLLGFGLDMSGDNAFYWVVLAWLAASFILLQ 174 Query: 279 RTIHSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVLSAALTGLAGSTKALILGFETLT 338 R + S FG L AI++NE RA ++GY RYKL AFV++A + G+AGS + F + Sbjct: 175 RLLASRFGLALNAIRQNERRAQAIGYPALRYKLAAFVIAAVIAGIAGSLIGMSNLFASPK 234 Query: 339 DVHWSMSGLVVLMTLVGGLGTIVGPIVGAVIIIMLENKLGDIGNWLASVTHIEWFNTLGD 398 +HWS SG++++M +GG+G G ++GA ++LE L TH E++ Sbjct: 235 LLHWSQSGILLVMVALGGVGYFYGGLLGAAAFLLLEEVLS---------THFEYWQ---- 281 Query: 399 SVTIVTGFIFIVCVLAFRRGI 419 I G I ++ V+ RG+ Sbjct: 282 ---IYVGLILLLVVMVAPRGV 299 Score = 113 bits (282), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 70/155 (45%), Positives = 101/155 (65%), Gaps = 2/155 (1%) Query: 8 ILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQGIPRGK 67 +L L AL+GLV+G++A+R + +YF MIT+ALAQ+ ++ + + GG+DGLQ R Sbjct: 86 LLAALVTALVGLVIGVIALRTRAVYFIMITMALAQLFYYFFVGFRYYGGDDGLQVTAR-P 144 Query: 68 LLGF-IDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVDR 126 LLGF +D+S D Y+VVLA A F L+ R + S FG L AI++NE RA ++GY R Sbjct: 145 LLGFGLDMSGDNAFYWVVLAWLAASFILLQRLLASRFGLALNAIRQNERRAQAIGYPALR 204 Query: 127 YKLLAFVLSAALTGLAGSTKALILGFETLTDVHWS 161 YKL AFV++A + G+AGS + F + +HWS Sbjct: 205 YKLAAFVIAAVIAGIAGSLIGMSNLFASPKLLHWS 239 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 72/187 (38%), Positives = 112/187 (59%), Gaps = 12/187 (6%) Query: 206 LAIRRQGIYFTMITLALAQMLFFVAL-QAPFTGGEDGLQGIPRGKL----LGFIDLSSDM 260 +IR++G YF M+TLA AQM +F+A TGG++GL IPR L L + +S Sbjct: 100 FSIRQRGTYFVMLTLAFAQMFYFLAYTMKDVTGGDNGLLDIPRPPLALFGLTLLPTTSSW 159 Query: 261 TLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVLSAAL 320 Y VV + + F L+ R + S G+ L A++ENE RA +LGYDV KL AFV+S A+ Sbjct: 160 QYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARASALGYDVRLLKLAAFVISGAV 219 Query: 321 TGLAGSTKALILGFETLTDVHWSMSGLVVLMTLVGGLGTIVGPIVGAVIIIMLENKLGDI 380 TGLAG A++ G L+++ + S +++MT++GG G + ++GA ++ + Sbjct: 220 TGLAGGLHAMMTGVAPLSNIEYHTSEAILVMTVIGGTGNLFASVLGASFYVL-------V 272 Query: 381 GNWLASV 387 GNWL+++ Sbjct: 273 GNWLSTL 279 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 61/147 (41%), Positives = 87/147 (59%), Gaps = 5/147 (3%) Query: 24 LAIRRQGIYFTMITLALAQMLFFVAL-QAPFTGGEDGLQGIPRGKL----LGFIDLSSDM 78 +IR++G YF M+TLA AQM +F+A TGG++GL IPR L L + +S Sbjct: 100 FSIRQRGTYFVMLTLAFAQMFYFLAYTMKDVTGGDNGLLDIPRPPLALFGLTLLPTTSSW 159 Query: 79 TLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVLSAAL 138 Y VV + + F L+ R + S G+ L A++ENE RA +LGYDV KL AFV+S A+ Sbjct: 160 QYYTVVAILFVLVFWLLQRVVDSVLGKTLLAVRENEARASALGYDVRLLKLAAFVISGAV 219 Query: 139 TGLAGSTKALILGFETLTDVHWSGHAA 165 TGLAG A++ G L+++ + A Sbjct: 220 TGLAGGLHAMMTGVAPLSNIEYHTSEA 246 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 101 bits (252), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 73/217 (33%), Positives = 110/217 (50%), Gaps = 10/217 (4%) Query: 162 GHAAFFGGAGYVAGQAIKAWGVPTEIGLILGTLTGALIGLVMGLLAIRRQGIYFTMITLA 221 GHAA+FG Y A P + ++ G T L L+ G +R G+Y M+TLA Sbjct: 377 GHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAMLAALLFGWFCVRLSGVYLAMLTLA 436 Query: 222 LAQMLFFVALQ-APFTGGEDGLQGIPRGKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRT 280 AQ+++ Q TGG +GL G+ L+S + YY+ L + G L+ R Sbjct: 437 FAQIVWAAIYQWDDLTGGSNGLVGLRTAA-----ALASPLAYYYLALLCSALGVYLLRRV 491 Query: 281 IHSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVLSAALTGLAGSTKALILG--FETLT 338 IH+PFG L+A ++ RA +LG DV + F L+ GLAG A G + Sbjct: 492 IHAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAGVLFAFSKGSISPEVA 551 Query: 339 DVHWSMSGLVVLMTLVGGLGTIVGPIVGAVIIIMLEN 375 V+ S+ GLV M L+GG+ ++ GP++GA + L++ Sbjct: 552 GVNRSVDGLV--MVLLGGVQSLAGPLLGASVFTWLQD 586 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 49/143 (34%), Positives = 73/143 (51%), Gaps = 6/143 (4%) Query: 2 PTEIGLILGTLTGALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQ-APFTGGEDGL 60 P + ++ G T L L+ G +R G+Y M+TLA AQ+++ Q TGG +GL Sbjct: 399 PMPLAMLAGAGTAMLAALLFGWFCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGL 458 Query: 61 QGIPRGKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISL 120 G+ L+S + YY+ L + G L+ R IH+PFG L+A ++ RA +L Sbjct: 459 VGLRTAA-----ALASPLAYYYLALLCSALGVYLLRRVIHAPFGLALRAARDASGRAQAL 513 Query: 121 GYDVDRYKLLAFVLSAALTGLAG 143 G DV + F L+ GLAG Sbjct: 514 GMDVRALQWGGFALAGLFCGLAG 536 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 61/219 (27%), Positives = 115/219 (52%), Gaps = 2/219 (0%) Query: 162 GHAAFFGGAGYVAGQAIKAWGVPTEIGLILGTLTGALIGLVMGLLAIRRQGIYFTMITLA 221 GHA FG Y AG G+P + + A+ G ++ L A+R G Y M+TLA Sbjct: 58 GHAGLFGIGSYAAGVLFFKLGMPIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVTLA 117 Query: 222 LAQMLFFVALQAPFTGGEDGLQGIPRGKLLGFIDLSSDMTLYYVVLAIAIAGFALIVRTI 281 ++ + + F +G GI K + D S++ +Y+V + + ++ R + Sbjct: 118 FGTIIQILINEMTFL--TEGPLGIKLQKPMIGGDKMSEVQFFYLVAVLMVLSMLVVHRIL 175 Query: 282 HSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVLSAALTGLAGSTKALILGFETLTDVH 341 S G+ +A++++ + +G V RYK+ AF++SAAL GL+G+ A + + + Sbjct: 176 KSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISPNTYN 235 Query: 342 WSMSGLVVLMTLVGGLGTIVGPIVGAVIIIMLENKLGDI 380 + ++ + +L ++GG + +G ++GA+I++ML + L DI Sbjct: 236 FELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSDI 274 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 39/133 (29%), Positives = 72/133 (54%), Gaps = 2/133 (1%) Query: 15 ALIGLVMGLLAIRRQGIYFTMITLALAQMLFFVALQAPFTGGEDGLQGIPRGKLLGFIDL 74 A+ G ++ L A+R G Y M+TLA ++ + + F +G GI K + D Sbjct: 93 AVFGAILALPALRVTGPYLAMVTLAFGTIIQILINEMTFL--TEGPLGIKLQKPMIGGDK 150 Query: 75 SSDMTLYYVVLAIAIAGFALIVRTIHSPFGQVLKAIKENEPRAISLGYDVDRYKLLAFVL 134 S++ +Y+V + + ++ R + S G+ +A++++ + +G V RYK+ AF++ Sbjct: 151 MSEVQFFYLVAVLMVLSMLVVHRILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFII 210 Query: 135 SAALTGLAGSTKA 147 SAAL GL+G+ A Sbjct: 211 SAALAGLSGALYA 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.144 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,073,119 Number of extensions: 39793 Number of successful extensions: 267 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 256 Number of HSP's successfully gapped: 36 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)