BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0186.p (365 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 116 3e-27 gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 99 4e-22 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 92 9e-20 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 89 4e-19 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 76 4e-15 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 96/342 (28%), Positives = 157/342 (45%), Gaps = 15/342 (4%) Query: 9 KAVVCHGPKDYRLQTIRCPQAGRNELVIRIGACGICASDCKCHSGAAMFWGGDGQDSWVK 68 + +V H P D R+Q + +L +++ A GIC SD + GG G +K Sbjct: 3 QGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYY-----HHGGFGV-VRIK 56 Query: 69 PPVVAGHEFFGYVAELGEGAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWMCEVHNIF 128 P+V GHE G +AE+G + + G R+ PC AC +C+ GK C + Sbjct: 57 EPMVLGHEVSGQIAEVGTAVTD---IAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFY 113 Query: 129 GF-QREVADGGMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHTVNRGDIQLDDVV 187 G R G + + H + + +AA+ EPLS A+H V R + V Sbjct: 114 GSAMRTPHVQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRV 173 Query: 188 VIAGAGPLGLMMTQVAKLKTPRKLVVVDLVPERLALAREFGADVVIDPAREDALAVIKGL 247 ++ G GP+G ++ A+ ++VV D+ L A + GAD I+ A + A +K Sbjct: 174 LVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMA--ETPAALKAY 231 Query: 248 TGGYGC-DVYIETTGVPAGVTQGLQLIRRLGRFVEFSVFGKETSADWSIIGDRKELDVRG 306 G DV E +G + L+ +R V+ + G ++G KEL+ RG Sbjct: 232 AADKGTFDVLFEASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVG--KELEWRG 289 Query: 307 AHLGPYCYPVAIDLLARGLVTSKGIVTHSFPLEKWEEAFALA 348 A + +A++L+ R LV K ++T ++P+ + EAF +A Sbjct: 290 AFRFHEEFAMAVELMNRKLVDVKPLITATYPIAQAVEAFEIA 331 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 99.4 bits (246), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 95/345 (27%), Positives = 155/345 (44%), Gaps = 16/345 (4%) Query: 8 MKAVVCHGPKDYRLQTIRCPQA-GRNELVIRIGACGICASDCKCHSGAAMFWGGDGQDSW 66 M+A+V ++ +L+ I PQ G ++ IRI GIC SD ++ G Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTH------GSIGPFK 54 Query: 67 VKPPVVAGHEFFGYVAELGEGAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWMCEVHN 126 V+ P+V GHE G V E+G A H V GDRV E +P G Y + Sbjct: 55 VEAPMVLGHEASGTVIEVGS-AVSHLKV--GDRVCMEPGIPRLDSPATLRGMYNLDPAVR 111 Query: 127 IFGFQREVADGGMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHTVNRGDIQLDDV 186 + G + + P+ A +++P ++ + AI+EPLS + + ++ D Sbjct: 112 FWA--TPPIHGCLTGSVVHPA-AFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDT 168 Query: 187 VVIAGAGPLGLMMTQVAKLKTPRKLVVVDLVPERLALAREFGADVVIDPAREDALAVIKG 246 V+ GAG +G M A ++++ D+V E+LA + A + +D RE V++ Sbjct: 169 AVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQ 228 Query: 247 LTGGYGCDVYIETTGVPAGVTQG-LQLIRRLGRFVEFSVFGKETSADWSIIGDRKELDVR 305 T G+G DV E +G AGV Q L L+ G V + + D + KE+ + Sbjct: 229 ATDGWGADVVFEASG-HAGVYQTLLDLVCPGGCAVLVGMPPAPVALDV-VAMQTKEVRLE 286 Query: 306 GAHLGPYCYPVAIDLLARGLVTSKGIVTHSFPLEKWEEAFALANS 350 +P A+ L++ G++ K ++ FP + AF A S Sbjct: 287 SVFRYANIFPRALALISSGMIDVKPFISRKFPFSQSIRAFEEAAS 331 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 91.7 bits (226), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 91/314 (28%), Positives = 142/314 (45%), Gaps = 44/314 (14%) Query: 10 AVVCHGPKDYRLQTIRCPQAGRNELVIRIGACGICASDCKCHSGAAMFWGGDGQDSWVKP 69 AVV K ++ + P +++++ A G+C +D G D VKP Sbjct: 8 AVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHG----------DWPVKP 57 Query: 70 --PVVAGHEFFGYVAELGEGAAEHFGVQQGDRVIAEQI-VPCEACLFCKTGKYWMCEVHN 126 P + GHE GYVA +G G V++GDRV + C C C+TG +C Sbjct: 58 TPPFIPGHEGTGYVAAVGAGVKH---VKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQ 114 Query: 127 IFGFQREVADGGMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHTVNRG----DIQ 182 G+ +G AEY L V +P ++ AA P+ CA TV +G +++ Sbjct: 115 NTGYS---VNGSFAEY-GLADPKFVGHLPDNLDFGPAA---PVLCAGVTVYKGLKETEVR 167 Query: 183 LDDVVVIAGAGPLGLMMTQVAKLKTPRKLVVVDLVPERLALAREFGADVVIDPAREDALA 242 + VVI+G G LG M Q AK +V D+ ++LALA++ GAD+ +D +A+A Sbjct: 168 PGEWVVISGIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVA 226 Query: 243 VIKGLTGG-YGCDVYIETTGVPAGVTQGLQLIRRLGRF---------VEFSVFG---KET 289 ++ + GG +G V T P + Q +R G + VF K Sbjct: 227 EVQRIIGGAHGALV---TAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRI 283 Query: 290 SADWSIIGDRKELD 303 + SI+G R++L+ Sbjct: 284 TVRGSIVGTRQDLE 297 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 75/280 (26%), Positives = 119/280 (42%), Gaps = 36/280 (12%) Query: 8 MKAVVCHGPKDYRLQTIRCPQAGR-NELVIRIGACGICASDCKCHSGAAMFWGGDGQDSW 66 M+A++ H D R++T+ P +++++++ A IC SD + G + Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYRG---------KIPA 51 Query: 67 VKPPVVAGHEFFGYVAELGEGAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWMCEVHN 126 K + GHEF G V E G +++GDRV+ ++ C C FC Y CE N Sbjct: 52 TKAGDILGHEFMGVVVEAGPAIKR---LKKGDRVVVPFVIACGQCFFCDQHLYAACETTN 108 Query: 127 -----------------IFGFQREVAD--GGMAEYMRLPSNAVVHKIPMDMPLEDAAII- 166 +FG+ GG AEY+R+P A V I +D L D ++ Sbjct: 109 PDRGALMNAKGIRSGAAMFGYSHLYGGMPGGQAEYVRVP-KADVGPIRIDSALADEQVLF 167 Query: 167 --EPLSCAIHTVNRGDIQLDDVVVIAGAGPLGLMMTQVAKLKTPRKLVVVDLVPERLALA 224 + L V ++ V I GAGP+G M A++ ++ +VD RL A Sbjct: 168 LSDILPTGYQAVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFA 227 Query: 225 REFGADVVIDPAREDALAVIKGLTGGYGCDVYIETTGVPA 264 +E + ++ + D I T G G D I+ G A Sbjct: 228 QEQYDVIPVNFDQVDPAEYIIANTAGRGVDGVIDAVGFEA 267 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 75.9 bits (185), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 96/362 (26%), Positives = 148/362 (40%), Gaps = 48/362 (13%) Query: 27 PQAGRNELVIRIGACGICASDCKCHSGAAMFWGGDGQDSWVKPPVVAGHEFFGYVAELGE 86 P+AG E+++ I A GIC +D SGA D P + GHE G V ++G Sbjct: 25 PKAG--EVLVEIKATGICHTDYYTLSGA---------DPEGIFPAILGHEGAGVVVDVGP 73 Query: 87 GAAEHFGVQQGDRVIAEQIVPCEACLFCKTGKYWMCEVHNIF--------GFQREVADG- 137 G +++ D VI C C FC + K +C+ R DG Sbjct: 74 GVKS---LKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPDATSRFSLDGK 130 Query: 138 ---------GMAEYMRLPSNAVVHKIPMDMPLEDAAIIEPLSCAIHT-----VNRGDIQL 183 + Y+ +P A+ KI D P + I C + T + ++ Sbjct: 131 PLFHYMGTSTFSNYIVVPEIALA-KIRSDAPFDKVCYI---GCGVTTGVGAVLFTAKVEA 186 Query: 184 DDVVVIAGAGPLGLMMTQVAKLKTPRKLVVVDLVPERLALAREFGADVVIDPAR-EDALA 242 VV+ G G +GL + Q AK+ K++ VDL P R A+AR+FG I+P + + Sbjct: 187 GANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKDVPNVVD 246 Query: 243 VIKGLTGGYGCDVYIETTGVPAGVTQGLQLIRR---LGRFVEFSVFGKETSAD--WSIIG 297 I G+T G G D E G + Q L+ + + + G+E S + G Sbjct: 247 AIIGMTDG-GADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQLVTG 305 Query: 298 DRKELDVRGAHLGPYCYPVAIDLLARGLVTSKGIVTHSFPLEKWEEAFALANSVASIKVL 357 + G G P +D G + ++TH PLE+ E F L S SI+ + Sbjct: 306 RVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGESIRSV 365 Query: 358 LK 359 ++ Sbjct: 366 VE 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,244,768 Number of extensions: 56681 Number of successful extensions: 209 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 6 Length of query: 365 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 279 Effective length of database: 1,212,660 Effective search space: 338332140 Effective search space used: 338332140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)