BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0185.p (243 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 182 2e-47 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 112 3e-26 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 109 2e-25 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 107 1e-24 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 106 2e-24 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 182 bits (463), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 101/238 (42%), Positives = 145/238 (60%) Query: 3 FDVAKRKYIVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLREEIACETLALDIAD 62 A + I+TGA GIG A LL GA V+A+ R LA L+EEI +A+D++D Sbjct: 1 MSFAGKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVIAVDLSD 60 Query: 63 DGAMAVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMI 122 A A+ + D L+N +GI+VLE+ L ++ E ++ V+ VN R + + A I Sbjct: 61 PAAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRI 120 Query: 123 REGRSGSIVNVSSQAALIALDDHLAYCASKAAMDAITRVMCAELGPHGIRVNSVNPTVTL 182 +G G++VN++S A + HL Y ASKA ++ TRV+ ELGPHGIRVN+V PT+TL Sbjct: 121 AQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITL 180 Query: 183 TPMALQAWADPEKRRRILEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGGY 240 T +A AW+DP K ++ P +FA+PEDVA I LLS AAM++G +P+DGG+ Sbjct: 181 TELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 238 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 112 bits (280), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 123/241 (51%), Gaps = 12/241 (4%) Query: 4 DVAKRKY-IVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLREEIACETLAL---D 59 D+ K K +VTG T GIG AR L + GA V+A G EE A + L + Sbjct: 6 DLLKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPP----FAHEEFAGSEVTLVEMN 61 Query: 60 IADDGAMAVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAA 119 +A + ++ AL + +D +VNC+GI ++ L +E F++V+ VN GT M A Sbjct: 62 VASEESVRAALAGLTQLDIVVNCAGI--IKRGAELETEVFEQVIAVNLTGT-MRVCAAAR 118 Query: 120 TMIREGRSGSIVNVSSQAALIALDDHLAYCASKAAMDAITRVMCAELGPHGIRVNSVNPT 179 ++R+ R G IVN +S + Y ASK + +T+ + GIRVN+V P Sbjct: 119 ELLRQSR-GCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 180 VTLTPMALQAWADPEKRRRILEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGG 239 TP+ DP + IL PL ++ PEDV + FL S AA+ ++G +P+DGG Sbjct: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 Query: 240 Y 240 Y Sbjct: 238 Y 238 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 127/241 (52%), Gaps = 16/241 (6%) Query: 11 IVTGAT--SGIGEVTARLLRSCGAAVVAVGRQKDKLALLREEIACETLAL--DIAD---- 62 ++TG +G+G TARL+ GA VV + + A I + L L D+ D Sbjct: 9 VITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADVTDKASC 68 Query: 63 DGAMAVALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMI 122 + A A LQ ID LVN +GI+ +L +T ++DR++ V+ RGT MS+ V M Sbjct: 69 ETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQAVLPAM- 127 Query: 123 REGRSGSIVNVSSQAAL----IALDDHLAYCASKAAMDAITRVMCAELGPHGIRVNSVNP 178 R ++GSI+ +SS +A I H Y A+KA + + R M E GP GIRVNS+ P Sbjct: 128 RAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFGPEGIRVNSITP 185 Query: 179 TVTLTPMALQAWADPEKRRRILEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDG 238 + T + ++ EK+ I E IPL + +D+ +FL SD + +G++L ++G Sbjct: 186 GLIGTDI-IKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGITLDVNG 244 Query: 239 G 239 G Sbjct: 245 G 245 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 81/236 (34%), Positives = 120/236 (50%), Gaps = 9/236 (3%) Query: 11 IVTGATSGIGEVTARLLRSCGAAVVAVGRQKDKLALLREEIACETLA---LDIADDGAMA 67 IVTGA SGIGE TAR GA V+ R L + + + E A D++ + Sbjct: 18 IVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDVSHHEQVR 77 Query: 68 ----VALQDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAMMSRHVAATMIR 123 A++ +D LV+ +G+ N ++ E++ RV N G +R + Sbjct: 78 QLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAREALPHL-- 135 Query: 124 EGRSGSIVNVSSQAALIALDDHLAYCASKAAMDAITRVMCAELGPHGIRVNSVNPTVTLT 183 E G IVNV+S + L A + AY ASK A+ +TR M + G G+R+N+V P++T T Sbjct: 136 EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPSLTHT 195 Query: 184 PMALQAWADPEKRRRILEAIPLRKFAAPEDVAAPILFLLSDAAAMISGVSLPIDGG 239 M DP + E I L + A P ++AA I FL S A+ ++GV+LP+DGG Sbjct: 196 AMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 77/248 (31%), Positives = 124/248 (50%), Gaps = 15/248 (6%) Query: 4 DVAKRKYIVTGATSGIGEVTARLLRSCGAAVV-------AVGRQKDKLALLREEIACETL 56 D R +VTG GIG ++ L + G V+ A + D L A L Sbjct: 5 DSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA--PL 62 Query: 57 ALDIADDGAMAVAL----QDVRGIDGLVNCSGISVLENSLALTSENFDRVMQVNARGTAM 112 +D+ ++A A +D D LVN +G++ + L EN+ + M VN G + Sbjct: 63 QMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLL 122 Query: 113 MSRHVAATMIREGRSGSIVNVSSQAALIALDDHLAYCASKAAMDAITRVMCAELGPHGIR 172 +H A M+++ + G IVN+SS + + A AY SKAA+ +TR M EL +GI Sbjct: 123 AGQHAARLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGIT 181 Query: 173 VNSVNPTVTLTPMALQAWADPEKRRRILEAIPLRKFAAPEDVAAPILFLLSDAAAMISGV 232 VNSV P TPM QA + R +P+R++ +++A+ + +L ++ +A I+G Sbjct: 182 VNSVAPGPVDTPMT-QAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGH 240 Query: 233 SLPIDGGY 240 +P+DGG+ Sbjct: 241 VIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 706,311 Number of extensions: 27086 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 47 Length of query: 243 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 160 Effective length of database: 1,226,865 Effective search space: 196298400 Effective search space used: 196298400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)