BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0178.p (208 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba... 281 3e-77 gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba... 76 2e-15 gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba... 63 1e-11 >gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 210 Score = 281 bits (718), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 160/196 (81%), Positives = 180/196 (91%) Query: 1 MQALFHMIGITDVWQLIAATVVFLMLPGPGTFCVLTCSAKHGLRGGFAAIAGLMLGDIVL 60 MQALFH +GITD+WQLIAAT+VFL+LPGPGTFCVLTC+AK G+RGGFAA AGLMLGD+VL Sbjct: 1 MQALFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVL 60 Query: 61 MFLAAIGVAALLHANPLLFKGMQYVGAAYLAYLGARLLFAKEEGGSSVVPFSNMADFRRG 120 MFLAA GVAALLHANPLLF G+QY+GAAYLAYLGARLLFA+ EGG +VVPFSN A+FRRG Sbjct: 61 MFLAAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAEFRRG 120 Query: 121 FFVTLMNPKAIVFYMAFFPLFIDPATQQGAVTFLTMGAVICTCTLIYGSLLVLAGNAAAK 180 F VTL NPKAIVFYMAFFPLF+DPATQQGA+TF+TMGAVI CT +YGS+LV+ GNAAA+ Sbjct: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180 Query: 181 RLARNRKIATLASRAA 196 R+ARNR IA LASRAA Sbjct: 181 RMARNRTIARLASRAA 196 >gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 208 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 54/179 (30%), Positives = 84/179 (46%), Gaps = 11/179 (6%) Query: 10 ITDVWQLIAATVVFLMLPGPGTFCVLTCSAKHGLRGGFAAIAGLMLGDIVLMFLAAIGVA 69 + D+ + A + + PGP VLT G + G A AG G + LA +G A Sbjct: 1 MPDLILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFA 60 Query: 70 ALLHANPLLFKGMQYVGAAYLAYLGARLL--------FAKEEGGSSVVPFSNMADFRRGF 121 AL+ A P + +QY GAAYLAYLG R L A + G++ VP + + + Sbjct: 61 ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASAGLALAADGQGAAAVPLRRI--YWQSV 118 Query: 122 FVTLMNPKAIVFYMAFFPLFIDPATQQGAVTFLTMGAV-ICTCTLIYGSLLVLAGNAAA 179 ++NPK +F++AF P F+ A+ L + V I I+ ++ + +G A Sbjct: 119 LANMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSGAVGA 177 >gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 219 Score = 63.2 bits (152), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 61/199 (30%), Positives = 91/199 (45%), Gaps = 25/199 (12%) Query: 18 AATVVFLMLPGPGTFCVLTCSAKHGLRGGFAAIAGLMLGDIVLMFLAAIGVAALLHANPL 77 AA V + PG G ++ +G+R A I GL + +V++ +A GV +LL A+ Sbjct: 11 AAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAGVGSLLVASET 70 Query: 78 LFKGMQYVGAAYLAYLG---ARLLFAKEEGGSSV-------VPFSNMADFRR---GFFVT 124 F ++ GA YL YLG R EE G ++ + ++R GF Sbjct: 71 AFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTGFLTN 130 Query: 125 LMNPKAIVFYMAFFPLFIDPATQQGAVTFLTMGAVICTCTLIYGSLLVLAGNA-AAKRLA 183 NPK IVF +A P FID L + ++ T++ L+V+ G A AA RL Sbjct: 131 ATNPKGIVFMVAVLPQFIDHDRP------LWLQLLVLAVTMVAVDLVVMHGYAFAASRLQ 184 Query: 184 RNRKIATLASRAAPRARAR 202 R + S+ A RA+ R Sbjct: 185 RFFR-----SQKAIRAQNR 198 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.142 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 507,705 Number of extensions: 18834 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 4 Length of query: 208 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 127 Effective length of database: 1,236,335 Effective search space: 157014545 Effective search space used: 157014545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 128 (53.9 bits)