BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0161.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 223 1e-59 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 99 5e-22 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 96 2e-21 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 87 2e-18 gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 78 7e-16 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 223 bits (568), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 123/256 (48%), Positives = 163/256 (63%) Query: 6 RKIAVITGASSGIGAVYADRFAKRGYDLVLVARRADRLQALAATISTTHGVSVETLVADL 65 R A++TGASSGIGA+YADR A+ GYDL+LVAR RL LA I+ G SVE L ADL Sbjct: 10 RGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGRSVEVLEADL 69 Query: 66 ATDDGQASVAQVLTTNPAVSVLVNNAGIARLAPIAGGALEDSTSQIALNITALTRLTHAA 125 A V + L ++ ++++LVNNAGI P+ +E T IALN+TA TRLT+AA Sbjct: 70 GQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVEHMTGMIALNVTAPTRLTYAA 129 Query: 126 LPGMKARNEGLIINISSALALHSLPISSVYSGTKAFVLSFSRGLQQELADTAVKVQVVLP 185 +PG AR +G +INISS + + ++ VY G+KAFVL+FS+ L ELA V+VQ VLP Sbjct: 130 VPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAVLP 189 Query: 186 AATATEVWDQSGVPLSALNPDTVMPTEAMVDAALAALDQGESITLPAVADAGAIERFESA 245 ATAT+ W G+P+ L+P VM E MVDAAL + E +T+P++ + A +E+A Sbjct: 190 GATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRRELVTIPSLHEEDAWTGYEAA 249 Query: 246 RAALFAGTQTGKVAPR 261 R AL A APR Sbjct: 250 RQALIARLSANTPAPR 265 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 98.6 bits (244), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 75/199 (37%), Positives = 109/199 (54%), Gaps = 5/199 (2%) Query: 1 MSTSTR-KIAVITGASSGIGAVYADRFAKRGYDLVLVARRADRLQALAATISTTHGVSVE 59 MS + + K+ VITGASSG+G A A G LVL ARR +RL+ L A I+ G ++ Sbjct: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAI- 59 Query: 60 TLVADLA-TDDGQASVAQVLTTNPAVSVLVNNAGIARLAPIAGGALEDSTSQIALNITAL 118 + D+A DD +A VA+ + VLVNNAGI LAP+A +E+ I +N+ + Sbjct: 60 AVTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGV 119 Query: 119 TRLTHAALPGMKARNEGLIINISSALALHSLP-ISSVYSGTKAFVLSFSRGLQQELADTA 177 AALP A++ G IIN+SS + + +VYS TK V + + GL+ E D Sbjct: 120 LYGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPD-G 178 Query: 178 VKVQVVLPAATATEVWDQS 196 V+ ++ P A +E+ S Sbjct: 179 VRTTIISPGAVDSELKTHS 197 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 96.3 bits (238), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 63/181 (34%), Positives = 91/181 (50%), Gaps = 5/181 (2%) Query: 8 IAVITGASSGIGAVYADRFAKRGYDLVLVARRADRLQALAATISTTHGVSVETLVADLAT 67 + +ITGA++G GA A +FA G+ ++ RR +RL L + V LV D+ + Sbjct: 2 VVLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD---KVLPLVLDVTS 58 Query: 68 DDG-QASVAQVLTTNPAVSVLVNNAGIA-RLAPIAGGALEDSTSQIALNITALTRLTHAA 125 + +A + V VL+NNAG+A L P AL D + I N+ L +THA Sbjct: 59 KSSIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHAL 118 Query: 126 LPGMKARNEGLIINISSALALHSLPISSVYSGTKAFVLSFSRGLQQELADTAVKVQVVLP 185 LP M R G IIN+ S + P +VY TKAFV F+ L+ +L T V+ + P Sbjct: 119 LPDMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 Query: 186 A 186 Sbjct: 179 G 179 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 63/181 (34%), Positives = 93/181 (51%), Gaps = 14/181 (7%) Query: 7 KIAVITGASSGIGAVYADRFAKRGYDLVLVAR--RADRLQALAATISTTHGVSVETLVAD 64 K ITG SSG G A F +R + ++ R R D L A + H + L D Sbjct: 2 KTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPA------SPH---LRILPLD 52 Query: 65 LATDDGQASVAQVLTTNPAVSVLVNNAGIARLAPIAGGALEDSTSQIALNITALTRLTHA 124 +A D + A V P V VLVNNAGI L + + N +T A Sbjct: 53 VA--DAHSIQAAVEAAGP-VDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQA 109 Query: 125 ALPGMKARNEGLIINISSALALHSLPISSVYSGTKAFVLSFSRGLQQELADTAVKVQVVL 184 +P M+AR +GL+IN++S++ L +LP+ +VY+ +KA + +F+ L ELA V+V++VL Sbjct: 110 FIPQMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVL 169 Query: 185 P 185 P Sbjct: 170 P 170 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 78.2 bits (191), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 72/232 (31%), Positives = 118/232 (50%), Gaps = 15/232 (6%) Query: 5 TRKIAVITGASSGIGAVYADRFAKRGYDLVLVARRADRLQALAATISTTHGVSVETLVAD 64 T ITG +SGIG A+ F K G +++ RR L + A ++ + VAD Sbjct: 4 TDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFD--VAD 61 Query: 65 LATDDGQASVAQVLTTNPAVSVLVNNAGIARLAPIAGGALEDSTSQIAL--NITALTRLT 122 A+ D A+ A + P ++VLVNNAGI A G ++D ++ L N+ RLT Sbjct: 62 PASID--AAAATLKARYPGLNVLVNNAGIMPFDD-ASGHIDDEVARRILDTNLLGPIRLT 118 Query: 123 HAALPGMKARNEGLIINISSALALHSLPISSVYSGTKAFVLSFSRGLQQELADTAVKVQV 182 A + +K + I++ +S LA L +++VYS +KA + S++ + L +T+V+VQ Sbjct: 119 SALIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQE 178 Query: 183 VLPAATATEVWDQSGVPLSALNPDTVMPTEAMVDAALAAL-DQGESITLPAV 233 + P T++ +SG P + MP +A + +A L + E I + AV Sbjct: 179 IAPPWVDTDLVKKSGDPRA-------MPLDAFITETMARLASEDEEIIVDAV 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 739,397 Number of extensions: 28247 Number of successful extensions: 258 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 42 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)