BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0160.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 140 2e-34 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 121 9e-29 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 119 3e-28 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 86 6e-18 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 85 8e-18 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 140 bits (353), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 113/360 (31%), Positives = 168/360 (46%), Gaps = 52/360 (14%) Query: 1 MKALVLKSYGGLDKVDFAEIPRPVIKPDEILVQVLAAGLNPIDYMIAKGTFKPILP---- 56 MKA+ ++GG + + + + P P ++P E+LV+V A GLNP D+ + +G +K + P Sbjct: 6 MKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREG-YKMLPPEWQP 64 Query: 57 -LRLPHTVGSDLAGIVVEAGSKVTRFKPGDAVFASI---FDLPGGS--LAEFAAVPEYAA 110 + P +G+D++G+V V F GD V++ + L GGS A++ +V Sbjct: 65 TVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEI 124 Query: 111 ALKPANLDFVQAASIPMVGLTSWQAL---------------HERAQLKPGQKVFIPAGSG 155 ALKP + QAA+ PM LT+WQ + HE LK + V + +G Sbjct: 125 ALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLK-DKTVVVNGAAG 183 Query: 156 GIGTFAIQLAKHFGATVGTTTSSVNVNLVRSLGADEVVDYKKQDVENVLRGYDAVLGTVR 215 G+G FA+QLAK GA V S + +R LGADE +DY + E+VL D V+ V Sbjct: 184 GVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVIDAVG 243 Query: 216 GDALEKSLAILKPGSKVVSLIGPPDAAFARAXFARGMNFLMKVVFSLLSAKIIRRAKRRD 275 G A + L LK G + + F G + A R Sbjct: 244 GPASGRFLRTLKRGGALFPI------------FPLGFSG-------------AEEAARLG 278 Query: 276 ASYSFLFVRPDGGQLAEIGDLLASGRIRPVIDRVFAFDQAKDGLVHLEQGRAKGKVVVQM 335 + S VR G QL ++ LL SG +R ID FA A QG +GK+V+ + Sbjct: 279 VNVSTTQVRSSGAQLQQLAGLLDSGVVRVAIDSTFALQDAGKAHERAAQGHIRGKIVLSV 338 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 121 bits (303), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 84/247 (34%), Positives = 125/247 (50%), Gaps = 17/247 (6%) Query: 1 MKALVLKSYGG---LDKVDFAEIPRPVIKPDEILVQVLAAGLNPIDYMIAKGTFKPILPL 57 M+A+ YG L D E P P P + V + AAG+ P+D + G + ++ L Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLP---PGHVRVAMQAAGVAPVDTKLRAGRLQHLVAL 57 Query: 58 RLPHTVGSDLAGIVVEAGSKVTRFKPGDAVFASIFDLP-----GGSLAEFAAVPEYAAAL 112 LP G D AG++ G V + G+AV +P G +LAE +P+ Sbjct: 58 DLPKVPGRDGAGVITALGEGVHTLQVGEAVCV----MPSMAGRGTTLAELV-LPQEQVVR 112 Query: 113 KPANLDFVQAASIPMVGLTSWQALHERAQLKPGQKVFIPAGSGGIGTFAIQLAKHFGATV 172 KP L QAA++ ++W AL + A + G KVFI AGSG +G+ +QLA H GA V Sbjct: 113 KPDVLSMQQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEV 172 Query: 173 GTTTSSVNVNLVRSLGADEVVDYKKQDVENVLRGYDAVLGTVRGDALEKSLAILKPGSKV 232 + S NV V +LGA V+ Y + ++ V R D V+ V G A ++S +L+ G ++ Sbjct: 173 TASCRSSNVEYVAALGASRVLAYDRDELGRV-RDQDVVIDLVGGAAHDQSYRMLRRGGQL 231 Query: 233 VSLIGPP 239 V L+ P Sbjct: 232 VWLVAAP 238 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 105/351 (29%), Positives = 164/351 (46%), Gaps = 42/351 (11%) Query: 1 MKALVLKSYGGLDKVDFAEIPRPVIKPDEILVQVLAAGLNPIDYMIAKGTFKPILP--LR 58 M+A+ +K +GG + + E P P +K E+L++V AAG+N D G + P+ P Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNY-PVPPGASD 59 Query: 59 LPHTVGSDLAGIVVEAGSKVTRFKPGDAVFASIFDLPGGSLAEFAAVPEYAAALKPANLD 118 LP G ++AG +V + FK GD V A + GG AEF A P P L Sbjct: 60 LP---GLEVAGEIVAGDLGDSGFKLGDLVCALV---QGGGYAEFCAAPLAQCLPVPKGLS 113 Query: 119 FVQAASIPMVGLTSWQALHERAQLKPGQKVFIPAGSGGIGTFAIQLAKHFGATV-GTTTS 177 ++AA++P T W + +R QL + + + GS GIG AIQ+A+ G V T S Sbjct: 114 ALEAAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGS 173 Query: 178 SVNVNLVRSLGADEVVDYKKQDVENVL------RGYDAVLGTVRGDALEKSLAILKPGSK 231 + LGA+ ++YK +D E V+ RG D +L V GD L + + L + Sbjct: 174 AEKCRACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGR 233 Query: 232 VVSLIGPPDAAFARAXFARGMNFLMKVVFSLLSAKIIRRAKRRDASYSFLFVRPDGGQLA 291 +V + A+ A+G L +++RR R + S L RP + A Sbjct: 234 LVFI--------AQLGGAKG---------QLDMGQVMRR--RLTITGSTLRPRPVAFKAA 274 Query: 292 -------EIGDLLASGRIRPVIDRVFAFDQAKDGLVHLEQGRAKGKVVVQM 335 + L+ +G+IRPVI F +QA +E GK+++++ Sbjct: 275 IAAKLREHVWPLIEAGKIRPVIHHRFPLEQAAQAHAMMEGSTHIGKIMLEL 325 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 85.5 bits (210), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 92/357 (25%), Positives = 154/357 (43%), Gaps = 49/357 (13%) Query: 1 MKALVLKSYGGL----DKVDFAEIPRPVIKPDEILVQVLAAGLNPIDYMIAKGTFKPILP 56 MKA++ + L D + +P P ++LV+V A +NP+D I Sbjct: 1 MKAILTQPQSPLISAPDALIETTLPDPTPGEHDLLVEVKAISVNPVDTKIRASA------ 54 Query: 57 LRLPHTVGSDLAGIVVEAGSKVTRFKPGDAVF-ASIFDLPGGSLAEFAAVPEYAAALKPA 115 + +G D++G+V G VT F PGD VF A PG + +E V E KP+ Sbjct: 55 -KEARVLGWDVSGVVRAVGPAVTLFAPGDEVFYAGSITRPGAN-SELHVVDERIVGRKPS 112 Query: 116 NLDFVQAASIPMVGLTSWQALHERAQLKP----GQKVFIPAGSGGIGTFAIQLAKHFG-- 169 +L AA+IP+ +T+W+ L +R + GQ + I +GG+G+ QLA+ Sbjct: 113 SLGHADAAAIPLTAITAWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQL 172 Query: 170 ATVGTTTSSVNVNLVRSLGADEVVDYK---KQDVENV-LRGYDAVLGTVRGDA-LEKSLA 224 +GT + + V LGA V+D+ K +E + + V+ D + + Sbjct: 173 TVIGTASRPETAHWVGELGAHHVIDHSQPLKPQLEKLGIPEVGIVISLTHTDQHYAQIID 232 Query: 225 ILKPGSKVVSLIGPPDAAFARAXFARGMNFLMKVVFSLLSAKIIRRAKRRDASYSFLFVR 284 +L P + ++LI P + A + ++ +++F+ +F Sbjct: 233 VLAPQGQ-LALIDDPASLDAMPLKRKSISLHWELMFT-----------------RSMFQT 274 Query: 285 PD----GGQLAEIGDLLASGRIRPVIDRVFA-FDQAKDGLVH--LEQGRAKGKVVVQ 334 PD L + LL G +R + D A H LE G+AKGK+V++ Sbjct: 275 PDMIRQHELLQRVSQLLDQGVLRTTTGEHYGRIDAANLRRAHGLLESGKAKGKIVLE 331 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 85.1 bits (209), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 78/266 (29%), Positives = 121/266 (45%), Gaps = 16/266 (6%) Query: 2 KALVLKSYGGLDKVDFAEIPRPVIKPDEILVQVLAAGLNPIDYMIAKGTFKPILPLRLPH 61 KA+ + GG + +++ ++ E+ ++ A GLN ID G + P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLY----PQPLPA 58 Query: 62 TVGSDLAGIVVEAGSKVTRFKPGDAVFASIFDLPGGSLAEFAAVPEYAAALKPANLDFVQ 121 +G + AG + G+ V+ FK GD V + P G+ AE +P P + F Sbjct: 59 GLGQEAAGTIEAVGAGVSGFKVGDRV--AYAGRPNGAYAEERVMPADILVKLPDAISFDT 116 Query: 122 AASIPMVGLTSWQALHERAQLKPGQKVFIPAGSGGIGTFAIQLAKHFGAT-VGTTTSSVN 180 AA++ + G+T L + ++PG + A +GGIG A Q A+ G +GT +S Sbjct: 117 AAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEK 176 Query: 181 VNLVRSLGADEVVDYKKQD-VENVL-----RGYDAVLGTVRGDALEKSLAILKPGSKVVS 234 L GA V++YK ++ VE V RG V ++ D SL L+P +VS Sbjct: 177 AALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVS 236 Query: 235 L--IGPPDAAFARAXFA-RGMNFLMK 257 P A F + A RG FL + Sbjct: 237 FGNASGPVAPFGVSELASRGSLFLTR 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 953,658 Number of extensions: 36574 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 6 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)