BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0139.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 375 e-105 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 323 1e-89 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 323 2e-89 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 254 8e-69 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 254 1e-68 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 375 bits (964), Expect = e-105, Method: Compositional matrix adjust. Identities = 198/418 (47%), Positives = 281/418 (67%), Gaps = 6/418 (1%) Query: 20 DVTFRRVVWRIVPFVTLIWFLAWVDRVNVGFAKLTMMSDLQWSDAVYGAGAGIFFIGYFI 79 +VT+R++ WR++PF+ ++ LAW+DRVNVGFAKL M+ DLQ+S+AVYG GAGIFFIGYFI Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 80 FEVPSNLALQKFGARKTIMRITIGWGITCVLMAWVQTATQFYFLRFLMGAFEAGLQPGVI 139 FEVPSNL L+K GARKT+ RITI WG+ + MA+V T T FY LRFL+G FEAG PGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 140 LYLTYWLPTHRRGKALGIFVSASAVSLVVGSPLAAYIMELSQGLGGFKGWQWLFVXAGGR 199 LYLTYW P RR + G+F+++ A++ VG P+A IM +G+G WQWLF+ G Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 200 ---AGAAAYFLLTDRPRDAHWLNDREKEHVETELAAEDQALGPREHSFFASLRSGRMWAL 256 AG A L +RP +A WL++ EK V LAAE+ A G + SF + + R+W Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPG-KHFSFKDACTNYRVWIC 260 Query: 257 IMVFFCIIAGNATLVYYGPSLVKEVGFTDIKTLGWVMGGIYCCGWLGMLLNGYLSDRNKE 316 V+FC+++GNAT+ ++ PS++KE+G +G + + G L M+ NG+ SD+ E Sbjct: 261 AAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGE 320 Query: 317 VRIHTAVAAAIGALGLLLAAYFLGEKSAAGVVFALALSSAGTMGAIPVFWQLPGRFLSGT 376 R+H+A+A I +GL+ LG + A + +L L++ G + A PVFW +PG FL+GT Sbjct: 321 RRLHSAIATLIACIGLVATGMTLGNATLA--LCSLTLAAIGILAAFPVFWSIPGAFLAGT 378 Query: 377 AIVVGLAVINSVANLAGYFSPQLLGYLKTTTGQYGKGLTTIAIVELMATVMIFAFISK 434 A G+A+INS+ NLAG+ +P ++G LKT+TG GL +A +EL+A+ ++ FI K Sbjct: 379 AAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 323 bits (829), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 173/404 (42%), Positives = 247/404 (61%), Gaps = 9/404 (2%) Query: 19 RDVTFRRVVWRIVPFVTLIWFLAWVDRVNVGFAKLTMMSDLQWSDAVYGAGAGIFFIGYF 78 D T+ +V WR++P + L + +++DRVNVGFAKL M+ DL++SD VYG GAGIFF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 79 IFEVPSNLALQKFGARKTIMRITIGWGITCVLMAWVQTATQFYFLRFLMGAFEAGLQPGV 138 +FEVPSN+ L K GAR I RI I WGI MA+V T FY +RFL+G EAG PGV Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 139 ILYLTYWLPTHRRGKALGIFVSASAVSLVVGSPLAAYIMELSQGLGGFKGWQWLFVXAGG 198 ILYLTYW P RRGK +F++ A+S V+G PL+ +IM G+ G GWQW+F+ Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 199 RA---GAAAYFLLTDRPRDAHWLNDREKEHVETELAAEDQALGPREH-SFFASLRSGRMW 254 + G + DR RDA WL++ EK +E ++ E+ +EH S A +GR+W Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQ---KEHLSLGAMFANGRVW 253 Query: 255 ALIMVFFCIIAGNATLVYYGPSLVKEVGFTDIKTLGWVMGGIYCCGWLGMLLNGYLSDRN 314 +++FC + G + ++ P+++K G +D +G + Y M+L G +D Sbjct: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 Query: 315 KEVRIHTAVAAAIGALGLLLAAYFLGEKSAAGVVFALALSSAGTMGAIPVFWQLPGRFLS 374 +E R H A+ A IG +GLLL+ + G + + +L L+S G + +P+FW LP FLS Sbjct: 314 RERRWHVAIPAFIGCIGLLLSTQY-GHNTTLAMA-SLTLASVGILTTLPLFWSLPTAFLS 371 Query: 375 GTAIVVGLAVINSVANLAGYFSPQLLGYLKTTTGQYGKGLTTIA 418 GTA G+A+INS+ NLAG+ SP L+G+LK T G+ +A Sbjct: 372 GTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLA 415 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 323 bits (827), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 174/431 (40%), Positives = 260/431 (60%), Gaps = 6/431 (1%) Query: 3 TIAAAAPANIDTAAEERDVTFRRVVWRIVPFVTLIWFLAWVDRVNVGFAKLTMMSDLQWS 62 T A+ +D + +D +++V WRI+PF+ L + +A++DRVNVGFAKL M DL +S Sbjct: 2 TTASTTGVGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFS 61 Query: 63 DAVYGAGAGIFFIGYFIFEVPSNLALQKFGARKTIMRITIGWGITCVLMAWVQTATQFYF 122 + V+G GAG+FFIGYF+FEVPSN+ + K GAR I RI I WGI +VQ ATQFY Sbjct: 62 ETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYI 121 Query: 123 LRFLMGAFEAGLQPGVILYLTYWLPTHRRGKALGIFVSASAVSLVVGSPLAAYIMELSQG 182 LRFL+G EAG PG+ILYLTYW P+HRR K + +F+S V+ ++G+PL+ +IM+ Sbjct: 122 LRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQ 181 Query: 183 LGGFKGWQWLFVXAGGRA---GAAAYFLLTDRPRDAHWLNDREKEHVETELAAEDQALGP 239 GG +GWQW+F+ A G A L + + A WLND EK ++ ++ + + Sbjct: 182 NGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKG-KE 240 Query: 240 REHSFFASLRSGRMWALIMVFFCIIAGNATLVYYGPSLVKEVGFTDIKTLGWVMGGIYCC 299 +H F A ++ R+W + +++F + G L + P+LVK G +G + + C Sbjct: 241 SKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGC 300 Query: 300 GWLGMLLNGYLSDRNKEVRIHTAVAAAIGALGLLLAAYFLGEKSAAGVVFALALSSAGTM 359 + M L G +DR +E R H + A +G +G + AA F + + + +L+L++AG + Sbjct: 301 AIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVS--IASLSLAAAGVL 358 Query: 360 GAIPVFWQLPGRFLSGTAIVVGLAVINSVANLAGYFSPQLLGYLKTTTGQYGKGLTTIAI 419 P+FW LP FLSG A VG+A INSV NLAG+ SP L+GYLK T G+ +A+ Sbjct: 359 TCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAV 418 Query: 420 VELMATVMIFA 430 + ++ ++ A Sbjct: 419 MLVVGSIATLA 429 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 254 bits (650), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 154/413 (37%), Positives = 223/413 (53%), Gaps = 31/413 (7%) Query: 15 AAEERDVTFRRVVWRIVPFVTLIWFLAWVDRVNVGFAKLTMMSDLQWSDAVYGAGAGIFF 74 A++ D +R+V RI+PF+ + + ++++DR+N+GFA+L M DL +SDA+YG GA IF+ Sbjct: 17 ASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFY 76 Query: 75 IGYFIFEVPSNLALQKFGARKTIMRITIGWGITCVLMAWVQTATQFYFLRFLMGAFEAGL 134 +GY +FEVPSN+AL +FGAR+T RI + WG+ V M V A FY LRFL+G FEAG Sbjct: 77 VGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGF 136 Query: 135 QPGVILYLTYWLPTHRRGKALGIFVSASAVSLVVGSPLAAYIMELSQGLGGFKGWQWLFV 194 PG++LYL+YW P RR + IF + AV+ V+G ++ +IM G+ G GWQW+F Sbjct: 137 FPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFA 196 Query: 195 XAGGRA---GAAAYFLLTDRPRDAHWLNDREKEHVETE---------------LAAEDQA 236 G A G A FLL D P A WL+ E+ ++ LAA QA Sbjct: 197 IEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQA 256 Query: 237 LGPREHSFFASLRSGRMWALIMVFFCIIAGNATLVYYGPSLVKEVGFTDIKTLGWVMGGI 296 L R FA ++F + G+ TL ++ P ++++ G D+ + Sbjct: 257 LSHRTTYLFA-----------FIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIP 305 Query: 297 YCCGWLGMLLNGYLSDRNKEVRIHTAVAAAIGALGLLLAAYFLGEKSAAGVVFALALSSA 356 G +G++L SDR E H AV GAL L S A + L++++ Sbjct: 306 NAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLH--PSSFAVSLGLLSVAAV 363 Query: 357 GTMGAIPVFWQLPGRFLSGTAIVVGLAVINSVANLAGYFSPQLLGYLKTTTGQ 409 A+P+FW LP L I G+A I+SV +G SP L+G +KT TG Sbjct: 364 LIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGN 416 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 254 bits (649), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 156/428 (36%), Positives = 234/428 (54%), Gaps = 14/428 (3%) Query: 19 RDVTFRRVVWRIVPFVTLIWFLAWVDRVNVGFAKLTMMSDLQWSDAVYGAGAGIFFIGYF 78 D T ++V+ R++PF+ L + ++++DR+NVGFA LTM DL + + +G GAG+FFIGYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 79 IFEVPSNLALQKFGARKTIMRITIGWGITCVLMAWVQTATQFYFLRFLMGAFEAGLQPGV 138 FE+PSNLAL +FGAR I RI I WG+ + A+V F RFL+G EAG PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 139 ILYLTYWLPTHRRGKALGIFVSASAVSLVVGSPLAAYIMELSQGLGGFKGWQWLFVXAGG 198 LY T W P RGKA F+ V+ ++GSPL+ +MEL G+ GFKGWQ L + Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL-HGMWGFKGWQVLLLLEAL 198 Query: 199 RA---GAAAYFLLTDRPRDAHWLNDREKEHVETELAAEDQALGPRE-HSFFASLRSGRMW 254 A G FLL DRP A WL+ EK+ +E EL+ E L R + + + R++ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 255 ALIMVFFCIIAGNATLVYYGPSLVKEVGFTDIKTLGWVMGGIYCCGWLGMLLNGYLSDRN 314 AL FC I G+ ++ + P +++E G +G V Y G + M L L++R+ Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPS-HQVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 315 KEVRIHTAVAAAIGALGLLLAAYFLGEKSAAGVVFALALSSAGTMGAIPVFWQLPGRFLS 374 + A A + AL L ++A+ + + A+ ++ A + FW +P FL+ Sbjct: 318 ERRLFFVAGAIVLAALSLGISAFL---HTPLLKMLAITVAVASILSFQATFWAIPSGFLT 374 Query: 375 GTAIVVGLAVINSVANLAGYFSPQLLGYLKTTTGQYGKGLTTIAIVELMATVMIFAF--- 431 G A GLA+I S+ NL G+ P ++G+++ T + L +A L+ V+ A Sbjct: 375 GRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDP 434 Query: 432 --ISKTSK 437 + TSK Sbjct: 435 SRVPPTSK 442 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,164,641 Number of extensions: 42279 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 244 Number of HSP's successfully gapped: 17 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)