BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0125.p (355 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 288 6e-79 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 128 6e-31 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 122 3e-29 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 114 9e-27 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 114 2e-26 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 288 bits (736), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 154/341 (45%), Positives = 208/341 (60%), Gaps = 13/341 (3%) Query: 10 SEEERIAGQAAQWIVQLSADDEAARLTAQRDFDAWKAADPRHADMAARLEGFIGRVQGLR 69 + E I AA WIV+LSA D A + F W+ +HA A L+G + +GL Sbjct: 10 ASSEDITAMAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLL 69 Query: 70 GAGGKRHQPARAALNATFTLQHQPGPRTKQIKQIKQIKQVKRIGAAVAIALALGLPAWVA 129 + P A L A +PG + + + + GLP W+ Sbjct: 70 HSAQGDPSPLLAGLEAAMAPSPRPGRGRRAVAALALACCI-------------GLPLWLG 116 Query: 130 IKAFPPAYLAADMRTATGKWETRVLADGSRITLNSASAVNLHFDAARRTLELVQGEILVE 189 ++A+P YL ADMRTATG W+ R LADGSR+ L+ +SA N HFDA RRT+ELVQGE+LVE Sbjct: 117 LQAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVE 176 Query: 190 VARDPARPFVVETAHGSVRALGTRFVVSREADATVLSMLESRVEVRTAQQDPNHPTVVAA 249 VARDPARPFV++TA GS+RALGTRF+V R+ T++SMLES+V ++ P V+ A Sbjct: 177 VARDPARPFVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQPGADTTQAPLVLEA 236 Query: 250 GQRLRFTADNIGAIDDIDARSITDAWKFHQLVVRNQPLPEVLDALDRYRPGAISYNREQI 309 GQR+R G ++ IDA ++ AW+ HQLV +PL EVLD L+R+RPG I Y++ Q+ Sbjct: 237 GQRVRLHGGAAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQL 296 Query: 310 AAINVSAVLPLDDTGRALQLLANSFPALRFRTLSPYLVRVD 350 A ++++ VLPLDDT RALQLL + P LR R +PYL +D Sbjct: 297 AGLHMTVVLPLDDTQRALQLLVDGVPGLRVRQFTPYLTYID 337 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 128 bits (322), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 96/256 (37%), Positives = 131/256 (51%), Gaps = 16/256 (6%) Query: 100 IKQIKQIKQVKRIGAAVAIALALGLPAWVAIKAFPPAYLAADMRTATGKWETRVLADGSR 159 +KQ+ + K I +A AL LG W+A A A + TA G+ L DGSR Sbjct: 74 LKQVGPAGRRKAI-KLLAYALTLGPAGWLAYHALSGPEQGARIATAIGERREVQLDDGSR 132 Query: 160 ITLNSASAVNLHFDAARRTLELVQGEILVEVARD---PARPFVVETAHGSVRALGTRFVV 216 + LN+ + + L F A+R L L +GEI+V A D P RPF+V T GS+R LGTRF V Sbjct: 133 LWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQGSLRPLGTRFTV 192 Query: 217 SREADATVLSMLESRVEVRTAQQDPNHPTVVAAGQRLRFTADNIGA---IDDIDARSITD 273 A +++L+ VE+ A TV+ +G + RFT A DD A Sbjct: 193 RERDQAVEVAVLQGAVEITPASS--AQQTVLPSGMQTRFTRSGSAAPRPCDDTQA----- 245 Query: 274 AWKFHQLVVRNQPLPEVLDALDRYRPGAISYNREQIAAINVSAVLPLDDTGRALQLLANS 333 AW L+ PL + + L RYR G + + IA + VS V PL DT R L+LLA + Sbjct: 246 AWSRGLLIAERMPLAQFIGELGRYRSGLLQAD-PAIAGLEVSGVFPLADTDRVLRLLAQT 304 Query: 334 FPALRFRTLSPYLVRV 349 P +R + L+ Y VRV Sbjct: 305 LP-VRVQQLTRYWVRV 319 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 109/343 (31%), Positives = 164/343 (47%), Gaps = 29/343 (8%) Query: 9 VSEEERIAGQAAQWIVQLSADDEAARLTAQRDFDAWKAADPRHADMAARLEGFIGRVQGL 68 + ++E+I QA +W++QL D A F+ WKAADPRHA A L + + Sbjct: 1 MGKDEKIVEQAIEWMLQLEQGDLPPGERAA--FEQWKAADPRHAMAYANLSQSVQQFDVP 58 Query: 69 RGAGGKRHQPARAALNATFTLQHQPGPRTKQIKQIKQIKQVKRIGAAVAIALALGLPAWV 128 R G A L++ ++K+ K +K+I + + A L Sbjct: 59 RQVGA-----------APVILRNTLQQKSKRRKALKRIAALAGLAVGAAALLD------- 100 Query: 129 AIKAFPPAYLAADMRTATGKWETRVLADGSRITLNSASAVNLHFDAARRTLELVQGEILV 188 + P A L AD+ T TG+ + LADGS ITLN+ SA++ R + L++GEI V Sbjct: 101 --RVTPVAALTADLHTGTGQRQRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQV 158 Query: 189 EVARDPARPFVVETAHGSVRALGTRFVVSREADATVLSMLESRVEVRTAQQDPNHPTVVA 248 ++A A PF +ET HGSV A ++ + T L ++ + T ++ H T +A Sbjct: 159 QIAAANALPFSIETVHGSVSATAGTLMLGLQPLRTDLVAQQAAALLST--RNGQHAT-LA 215 Query: 249 AGQRLRFTADNIGAIDDIDARSITDAWKFHQLVVRNQPLPEVLDALDRYRPGAISYNREQ 308 AGQR F D IGA A ++W + L EV+ AL YR G + + Sbjct: 216 AGQRTWFDQDEIGAPR--AAGGAENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLD-PA 272 Query: 309 IAAINVSAVLPLDDTGRALQLLANSFPALRFRTLSPYLVRVDA 351 +A + +S PLDDT RAL LA++ P + +SPY V + Sbjct: 273 VAQLRISGAFPLDDTDRALSALASALP-ITVNRVSPYWVSISG 314 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 114 bits (286), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 123/357 (34%), Positives = 165/357 (46%), Gaps = 30/357 (8%) Query: 5 PEFDVSEEERIAGQAAQWIVQLSA--DDEAARLTAQRDFDAWKAADPRHADMAARLEGFI 62 P + ++ + QA W+V+L A D A Q AW+AADPRH + + L Sbjct: 2 PSNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASE 61 Query: 63 GRVQGLRGAGGKRHQPARAALNATFTLQHQPGPRTKQIKQIKQIKQVKRIGAAVAI-ALA 121 + Q L AL + H P R Q +Q Q ++ A + + A+A Sbjct: 62 EQFQRL------------VALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIA 109 Query: 122 LGLPAWVAIKAFPPAYLAADMRTATGKWETRVLADGSRITLNSASAVNLHFDAARRTLEL 181 LG A F A A T TG L DG+ + LN++S+V L FD RR L L Sbjct: 110 LGAVAARQAGLFE-ALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLL 168 Query: 182 VQGEILVEVARDPA----RPFVVETAHGSVRALGTRFVVSREADATVLSMLESRVEVRTA 237 QGEI ++ D RPF V+TAH ++ALGTRF V + D T L++LE RV V A Sbjct: 169 RQGEIFIDTGADAQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVA 228 Query: 238 QQDPNHPTVVAAGQRLRFTADNIGAIDDIDAR-SITD--AWKFHQLVVRNQPLPEVLDAL 294 D P V G+ + D G + AR S D AW LVVR L +V+ L Sbjct: 229 GVD---PLVAQPGEIVDIHPD--GRLVRRAARASAMDPTAWLEQSLVVRQMRLADVVAQL 283 Query: 295 DRYRPGAISYNREQIAAINVSAVLPLDDTGRALQLLANSFPALRFRTLSPYLVRVDA 351 RYR G + + +A VS V L DT AL+ LA + P L + + VRV A Sbjct: 284 ARYRSGWLQCD-PAVADWQVSGVFQLQDTDVALEALAQALP-LTIERRTRFWVRVAA 338 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 100/323 (30%), Positives = 151/323 (46%), Gaps = 31/323 (9%) Query: 14 RIAGQAAQWIVQLSADDEAARLTAQRDFDAWKAADPRHADMAARLEGFIGRVQGLRGAGG 73 ++A +A +W+++L A + ++AW++A P H R+E GR+Q L Sbjct: 19 QVAERALEWLLELQTQPLAP--STLEAWEAWRSAHPDHERAWRRIESVRGRLQAL----- 71 Query: 74 KRHQPARAAL-NATFTLQHQPGPRTKQIKQIKQIKQVKRIGAAVAIALALGLPAWVAIKA 132 P AAL A T G R + IK + A+ + G AW ++ Sbjct: 72 --ASPVNAALAQAALT---PSGNRRQVIKTL-------------ALLVFGGGVAWGLTRS 113 Query: 133 FPPAYLAADMRTATGKWETRVLADGSRITLNSASAVNLHFDAARRTLELVQGEILVEVAR 192 P AD+RT G+ VL DGS++ LN+ SAVNL F A R L L+ GE+LV + Sbjct: 114 EPWQQWRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGK 173 Query: 193 -DPARPFVVETAHGSVRALGTRFVVSREADATVLSMLESRVEVRTAQQDPNHPTVVAAGQ 251 RPF+VETA G ALGTR+ V + A T +S+ E V + +Q V+ AGQ Sbjct: 174 AGGGRPFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRL-VPRQAAQQARVLQAGQ 232 Query: 252 RLRFTADNIGAIDDIDARSITDAWKFHQLVVRNQPLPEVLDALDRYRPGAISYNREQIAA 311 + F+A +G + D + AW +V R L L L RY +S ++ Sbjct: 233 QAAFSAQGVGDVTTADETRM--AWTDGVIVARGMRLDAFLAELGRYTTDRLSCA-PAVSG 289 Query: 312 INVSAVLPLDDTGRALQLLANSF 334 + +S P+ D+ + LA + Sbjct: 290 VRISGSYPVADSDAVIATLAAAL 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 987,721 Number of extensions: 34848 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 5 Length of query: 355 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 269 Effective length of database: 1,212,660 Effective search space: 326205540 Effective search space used: 326205540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)