BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0121.p (283 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 401 e-113 gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 276 1e-75 gi|300311595|ref|YP_003775687.1| two component response regulato... 69 3e-13 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 67 1e-12 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/290 (70%), Positives = 230/290 (79%), Gaps = 7/290 (2%) Query: 1 MELKSLVDQPNKLPTVPKVVQQLIESFNSEDISAIEIAHLIAGDPALSAKLLRLANSAYF 60 MELKSLVDQP KLPTVPKVVQQL+ SFN+EDISA EIA IA DPALSAK+LRLANSA+F Sbjct: 1 MELKSLVDQPGKLPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFF 60 Query: 61 HVSRTVGTVDDALRMLGFVMVRNLVLGNGMVAAFKNTKGMELQQFWRYNLYTACAARWLA 120 H SRT+GTVDDALR+LGF+MVRNLVLGNGMVAAFKNT+GM+L QFWR+ LYTA AARWLA Sbjct: 61 HASRTIGTVDDALRILGFIMVRNLVLGNGMVAAFKNTRGMDLHQFWRHTLYTAVAARWLA 120 Query: 121 HQGG----DNGDAAFTLGMMHGIGQLQLHAVAAAEVALLDKQLNVLDAGRAELEKEKLGF 176 G DNGDA FT+GMMHGIGQLQLHAVAA VA LD+ NVLD+ RAE EKE GF Sbjct: 121 MHAGMVGEDNGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGF 180 Query: 177 HFADVSAELAKIWNFPETLSSALKDIPAPLASTPFDRMAAWVHLGAWRARAEVLDHSDED 236 H+ADVSAELA IW+FP+ L +AL+ +P PL + FDR AAWVHLG W AR +L DE+ Sbjct: 181 HYADVSAELAAIWHFPDALVTALRSVPDPLGAGRFDRAAAWVHLGGWAARVAILSQDDEN 240 Query: 237 MAASYPAAVAKRLGLDTGWALASAK---DHADKAMPPLKELTAGLDAMLE 283 A+YPAAV K+LG+D W K D A++AMP L EL AGLDAMLE Sbjct: 241 AQATYPAAVGKKLGVDPAWMPLLVKHDPDKAERAMPSLGELAAGLDAMLE 290 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 276 bits (706), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 139/282 (49%), Positives = 190/282 (67%), Gaps = 9/282 (3%) Query: 1 MELKSLVDQPNKLPTVPKVVQQLIESFNSEDISAIEIAHLIAGDPALSAKLLRLANSAYF 60 M L++L Q LP++PKVVQ++IESFN+E +S EIA +A D LSAK+LRLANS+Y+ Sbjct: 1 MNLEALFQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYY 60 Query: 61 HVSRTVGTVDDALRMLGFVMVRNLVLGNGMVAAFKNTKGMELQQFWRYNLYTACAARWLA 120 HVSRT+GTVDDA+ MLGF+ VR LV+ GM +FK G++L+ FWRY+L TA A+WLA Sbjct: 61 HVSRTIGTVDDAVLMLGFMTVRTLVVSTGMAGSFKKLPGVDLKLFWRYSLDTALVAKWLA 120 Query: 121 HQGGDNGDAAFTLGMMHGIGQLQLHAVAAAEVALLDKQLNVLDAGRAELEKEKLGFHFAD 180 +G N D AFT+G+MH IGQL +HA + +DK LDA R ++E++ G+ FAD Sbjct: 121 KRGKLNSDFAFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDFAD 180 Query: 181 VSAELAKIWNFPETLSSALKDIPAPLASTPFDRMAAWVHLGAWRARAEVLDHSDEDMAAS 240 V AELA+ W FP+ + ++ P PL FD AA VHL AWRARA +SDE++AA+ Sbjct: 181 VGAELARRWRFPDEFAKVIEAFPQPLQGE-FDPFAAIVHLAAWRARAHENQYSDEELAAT 239 Query: 241 YPAAVAKRLGLDTGWALASAKDHADKAMPPLKELTAGLDAML 282 +PA VA+RLG+ + + MPPL+EL G+ ++ Sbjct: 240 FPAEVAERLGVRL--------ETFTQEMPPLEELGEGMQELI 273 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 59/210 (28%), Positives = 93/210 (44%), Gaps = 25/210 (11%) Query: 13 LPTVPKVVQQLIESFNSEDISAIEIAHLIAGDPALSAKLLRLANSAYFHVSRTVGTVD-D 71 LP V ++++ EDI+ E+ + GDP L+ +L+++AN + R V +V+ + Sbjct: 19 LPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQIANIPNINKGRMVASVNVE 78 Query: 72 ALRMLGFVMVRNLVLGNGMVAAFKNTKGMELQQ--FWRYNLYTACAARWLAHQGGDNGDA 129 L M+G VR + L + + + E FW + ACA + L GD Sbjct: 79 LLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTAMACAGQAL----GDLLQV 134 Query: 130 A-----FTLGMMHGIGQLQLHAVAAAEVALL------DKQLNVLDAGRAELEKEKLGFHF 178 A FT+G++ GIGQL L + L D QL V E+E GF Sbjct: 135 APPAEMFTIGLLAGIGQLGLASARPKRYGELLLAGAKDAQLRV-------QERESFGFDH 187 Query: 179 ADVSAELAKIWNFPETLSSALKDIPAPLAS 208 ++A + + W+FP A+ P AS Sbjct: 188 LALAAAMMQDWHFPRIFCEAVLHHRDPAAS 217 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 67.4 bits (163), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 55/197 (27%), Positives = 92/197 (46%), Gaps = 14/197 (7%) Query: 36 EIAHLIAGDPALSAKLLRLANSAYFHVSRTVGTVD-DALRMLGFVMVRNLVLGNGM--VA 92 E+ HL+ DPALS +++R+ N R + +V D+L ++G VR L L + Sbjct: 42 ELIHLVQADPALSGRIIRIVNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETR 101 Query: 93 AFKNTKGMELQQFWRYNLYTACAARWLAHQ--GGDNGDAAFTLGMMHGIGQLQL-HAVAA 149 A + L FW ++ AC+A+ + Q G+ FT G++ IG+L L A Sbjct: 102 APASCANFSLPAFWAHSFAMACSAQAVTSQLRVAPLGE-MFTTGLLANIGKLTLASAQPT 160 Query: 150 AEVALLDKQLNVLDAGRAELEKEKLGFHFADVSAELAKIWNFPETLSSALKDIPAP---- 205 ALL + L+ R +E+ GF+ +++A L + W P+ A+ P Sbjct: 161 GYSALLTRALDA--PARRAMEQSLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIG 218 Query: 206 -LASTPFDRMAAWVHLG 221 +A+T D + +HL Sbjct: 219 EIAATRQDEIVWTLHLA 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 844,519 Number of extensions: 31974 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 4 Length of query: 283 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 199 Effective length of database: 1,222,130 Effective search space: 243203870 Effective search space used: 243203870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)