BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0119.p (785 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate) synthe... 1178 0.0 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 76 1e-14 >gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 832 Score = 1178 bits (3047), Expect = 0.0, Method: Compositional matrix adjust. Identities = 596/799 (74%), Positives = 671/799 (83%), Gaps = 19/799 (2%) Query: 1 MATRKTP-AATPEDRAATPQA-APVMGIPQLGDVNAYLTDAMQRTYLFLDTLLDRGNNYL 58 M RK P +A P D A P PV GDV AYL D +QR++LFLDTLLDRGN+YL Sbjct: 1 MINRKKPVSAAPNDGHAHPDDDQPVAASASWGDVPAYLQDCLQRSFLFLDTLLDRGNDYL 60 Query: 59 EHLDQGTPPLLKFEHEVVMDGHDLPNPCNYALLRLQPPASMPVKQEARPVVVVDPRAGHG 118 EHL+QGTPPLLKF HEVV+DGHDLP CNYALLRLQPPA +PV +ARPVVVVDPRAGHG Sbjct: 61 EHLEQGTPPLLKFAHEVVLDGHDLPQACNYALLRLQPPAGLPVNPQARPVVVVDPRAGHG 120 Query: 119 PGIGGFKFDSEVGMAMRAGHTVYFVTFRPEPEDGQTLLTVMDAEALFIEEIIRRHPQCPA 178 PGIGGFKFDSEVGMAM+AGHTVYFVTFRPEPEDGQTL+TVMDAEA F+EE+IRRHPQC A Sbjct: 121 PGIGGFKFDSEVGMAMKAGHTVYFVTFRPEPEDGQTLVTVMDAEARFLEEVIRRHPQCSA 180 Query: 179 KPVVIGNCQAGWAMMALNARRPELFGPLMIVGAPVSYWAGSSTLNPMRYSGAQLGGSWLA 238 KPVVIGNCQAGWAMM L+A RPELFGPLM+VGAP SYWAGSSTLNPMRYSGA LGG+WLA Sbjct: 181 KPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLNPMRYSGANLGGTWLA 240 Query: 239 SMTSDLGADRFDGAHLVENFEKLNPANTLWNKYYNLWAGVDTEAERFLEFERWWGGYFRM 298 S+ +DLGADRFDGA+LVENFEKLNPANT WNKYYNLWAGVD EA RFLEFERWWGG+FRM Sbjct: 241 SLAADLGADRFDGAYLVENFEKLNPANTFWNKYYNLWAGVDQEAARFLEFERWWGGFFRM 300 Query: 299 TGSEIESIVENLFVGNKLARGTMMAGDKAIDLRNITSPIVVFASWGDNITPPPQALNWII 358 TG+EIE+I ENLFVGNKLARG + K ++LR I++PIVVFASWGDNITPPPQALNWII Sbjct: 301 TGAEIEAITENLFVGNKLARGELQVRGKPVNLREISAPIVVFASWGDNITPPPQALNWII 360 Query: 359 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSIALKEHDQLVTSLDVIESLPPGLYEM 418 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGS+ALKEHDQLV+SLDVIESLPPGLYEM Sbjct: 361 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSVALKEHDQLVSSLDVIESLPPGLYEM 420 Query: 419 KLEAKAGKDEQRWDQLEPGDYTVHYQHRTMEDIRKINPEGREEEEMFSTIAQWSEFNANL 478 KLE K G+ QRWD LEPGDYTVHY+HRTMED+RK+NPEGREEE +FSTI+QWS+ NA Sbjct: 421 KLERKDGRPTQRWDVLEPGDYTVHYEHRTMEDLRKLNPEGREEEAIFSTISQWSQMNAQF 480 Query: 479 YKTYVRPWIKMSATREMANAMLKMSPLRMQRQLFSDSFFMAPFIRQKAAEARAERATVAD 538 YKT+VRPW++M+ATRE ANA+++M+PLRMQRQLFSD+ A FIRQ+AA+ARA+R + Sbjct: 481 YKTWVRPWVRMTATRESANALMRMNPLRMQRQLFSDAHPAAAFIRQQAAQARAQRVQLDP 540 Query: 539 DHPLKQLEKKMAQQITDELNLYRDRRDERTVKMTRQLFGPKGMGAWLKPHEPDAVVAHER 598 HPLKQ E +MAQ+ITDELN +RD+RD RTV+MTRQ+FGP G+GAWL P EPDA +A Sbjct: 541 QHPLKQYEARMAQKITDELNAWRDQRDARTVRMTRQMFGPNGLGAWLPPREPDAELAQRW 600 Query: 599 ALRELEIYREKALAHIAEGGFAEAVCRIVVAGMISIGAFERRSLRLARLLAQLP-MHASV 657 A +EL YRE + IAEGGFAEAVCRIV+AGMISIG FERRSLRLARLLAQLP MHASV Sbjct: 601 AQQELGRYRETVIGQIAEGGFAEAVCRIVIAGMISIGVFERRSLRLARLLAQLPNMHASV 660 Query: 658 SSKTNWVQLLKDQARXGPAAVAPVEALNALEQLLPDTATRERALAVSAAVMMIEPTLANP 717 S++TNWVQLLK+QAR AVAPVEALNAL Q+LPD A+RERALA+SAAVMMIEPTLANP Sbjct: 661 SAQTNWVQLLKEQARI--TAVAPVEALNALGQMLPDAASRERALALSAAVMMIEPTLANP 718 Query: 718 RSEIIEFLIGTLGVDPQRVIGLARKLTDAL-----------EKPEAKPAAAKRATKTTAM 766 RSEIIE LIGTL V+P +VI LARKLT A+ + EA P RA+ Sbjct: 719 RSEIIELLIGTLDVNPDKVIALARKLTSAIGAGSDEVTVPAGELEAAPVKRPRASSP--- 775 Query: 767 KKAAAKPKTAKSKATAKAA 785 ++ AKP ++ KAA Sbjct: 776 RRTEAKPAPTPARTQRKAA 794 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 75.9 bits (185), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 62/237 (26%), Positives = 102/237 (43%), Gaps = 36/237 (15%) Query: 182 VIGNCQAGWAMMALNARRPELFGPLMIVGAPVSYWAGSSTLN------PMRYSGAQLGGS 235 ++G CQ GW + AR PE L++ G+P+ AG L P+R+ Sbjct: 159 LVGLCQGGWISAMIAARFPEKVARLVLAGSPIDAGAGEGPLKQMVQRLPLRFY------- 211 Query: 236 WLASMTSDL---GADRFDGAHLVENFEKLNPANTLWNKYYNLWAGVDTEA--ERFLEFER 290 DL G G+ +++ ++ ++P +N++ L +D A + F Sbjct: 212 ------EDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELLQHIDDPAYLAKRETFAA 265 Query: 291 WWGGYFRMTGSEIESIVENLFVGNKLARGTMMAGDKAIDLRNITSPIVVFASWGDNITPP 350 W+ + G + ++F N LARG +A + +DLR IT P+ + A D+IT P Sbjct: 266 WYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQQLDLRQITCPLYLLAGERDDITTP 325 Query: 351 PQALNWIIDTWGDERAIAAAGRTIIYVLHESV--GHLGIFVGGSIALKEHDQLVTSL 405 Q LN A A G + + V GH+G+F+G + Q+ T L Sbjct: 326 EQVLN----------AAALVGTPAAQITQKIVPGGHIGLFMGSRTLAEVWPQIATWL 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,465,936 Number of extensions: 108830 Number of successful extensions: 339 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 2 Length of query: 785 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 692 Effective length of database: 1,179,515 Effective search space: 816224380 Effective search space used: 816224380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)