BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0050.p (321 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 444 e-126 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 178 6e-46 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 167 1e-42 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 130 1e-31 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 444 bits (1143), Expect = e-126, Method: Compositional matrix adjust. Identities = 228/317 (71%), Positives = 266/317 (83%) Query: 3 ELPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNAL 62 ELP + DV+ AA RI AH+TPVL SRTAD E+GAQ+FFKCENFQRMGAFKFRGAMNAL Sbjct: 9 ELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNAL 68 Query: 63 ARFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVR 122 +F QR GV+TFSSGNHAQ ALAA LG+PA IVMP+DAP AKVAATRGYG +VV Sbjct: 69 TQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 Query: 123 FNRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLGGG 182 ++RYTE+RE I LA + +T+IPPYDHP V++GQGTA EL +ETGPLDALFVCLGGG Sbjct: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 Query: 183 GLLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVGHY 242 GLLSG+ LAA+ALSP C+ YGVEP+AG+D QSLRAG+++HIDTPKTIADGAQTQH+G Y Sbjct: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 Query: 243 TFEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRIGV 302 TF +IR V DI TVSD+ELVA MRF+AERMKM+VEPTGCL FAA RK+KDQL+GKR+GV Sbjct: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 Query: 303 IVSGGNVDLAKLSGYFA 319 I+SGGNVDL KL+ + A Sbjct: 309 IISGGNVDLGKLATFLA 325 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 178 bits (451), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 120/308 (38%), Positives = 168/308 (54%), Gaps = 3/308 (0%) Query: 4 LPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNALA 63 + ND+ AAA R+ G +TP + SRT +GAQVF K EN Q +FK RGA N L Sbjct: 1 MTTLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLV 60 Query: 64 RFTPEQRN-DGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVR 122 GVV S+GNHAQ +A A LG+PATIVMP P K+ TRG+G VV Sbjct: 61 DVAASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 Query: 123 FNRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLGGG 182 E LAEK GLT + PYD +I+GQGT A E+ + LD L V +GGG Sbjct: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 Query: 183 GLLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVGHY 242 GLL+G +AA+A+ P+ + GV+ + +++ + H TIA+G G Sbjct: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKGTE--HPVGASTIAEGIAVGTPGTL 238 Query: 243 TFEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRIGV 302 T II R+ ++L V + ++ + E K +VE G AA+ + D+ +GKR+G+ Sbjct: 239 TRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGL 298 Query: 303 IVSGGNVD 310 ++SGGN+D Sbjct: 299 VLSGGNID 306 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 167 bits (422), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 111/318 (34%), Positives = 172/318 (54%), Gaps = 11/318 (3%) Query: 6 DYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNALARF 65 DY + A R+ A +TP+ + T I +++FK E+ Q + +FK RGA N +A Sbjct: 4 DYLKKILTA-RVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHL 62 Query: 66 TPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVRF-N 124 TP Q GV+ S+GNHAQ +AL+A LG A IVMP P+ K+ A + GG+VV F + Sbjct: 63 TPAQLKRGVICASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGD 122 Query: 125 RYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEE-TGPLDALFVCLGGGG 183 +T+ E T L +K LT + P+D P VI+GQGT E+ + P+ A+FV +GGGG Sbjct: 123 SFTDAYEHALT-LEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGG 181 Query: 184 LLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVGHYT 243 L++G KA+ PD + GV+ D +SL+AG+ + + +DG + VG T Sbjct: 182 LIAGVASYVKAVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEET 241 Query: 244 FEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKM-------KDQLK 296 F I + V +++ V D + ++ + + IVEP G L+ A + K +K Sbjct: 242 FRIAKELVDEVIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIK 301 Query: 297 GKRIGVIVSGGNVDLAKL 314 G+ + I G N++ +L Sbjct: 302 GESLITIACGANMNFDRL 319 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 100/310 (32%), Positives = 153/310 (49%), Gaps = 6/310 (1%) Query: 4 LPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAM---N 60 LP D+ AAG + T L + +GA+V+ K EN GAFK RG M + Sbjct: 9 LPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLH 68 Query: 61 ALARFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKV 120 LAR P+ GVV+ + GNH Q++ LAA LG+ ATIV+P+ K AA R G ++ Sbjct: 69 HLARQQPQL--SGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVEL 126 Query: 121 VRFNRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLG 180 + + E LA + L MIP H D+++G + ELF LD + V +G Sbjct: 127 IEHGSEFQESREYAQQLAAQQQLHMIPSL-HRDLVAGVASYWMELFAAQPGLDVVLVPIG 185 Query: 181 GGGLLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVG 240 G + G++ A AL + GV S AG+ I IADG + Sbjct: 186 QGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPD 245 Query: 241 HYTFEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRI 300 + E++ +V +++ V+DDE++ M+ + E G + AA +++ +L+G ++ Sbjct: 246 QASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRGLKV 305 Query: 301 GVIVSGGNVD 310 GV +SGGNVD Sbjct: 306 GVTLSGGNVD 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 999,165 Number of extensions: 40130 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 4 Length of query: 321 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 236 Effective length of database: 1,217,395 Effective search space: 287305220 Effective search space used: 287305220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)