BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0014.p (742 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 982 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 362 e-101 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 347 2e-96 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 335 1e-92 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 328 9e-91 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 982 bits (2538), Expect = 0.0, Method: Compositional matrix adjust. Identities = 499/731 (68%), Positives = 588/731 (80%), Gaps = 2/731 (0%) Query: 14 GPAPLASDFGFRQAIGTDDRETITSVLENHGARIGSTLSMLIGTDQSVKASTQAYPSTDL 73 G LASDFGFRQAIGTDDRETI SVL NHGARIG++++MLIG D ++AST YP TDL Sbjct: 67 GARLLASDFGFRQAIGTDDRETIASVLSNHGARIGASMAMLIGNDGQIRASTVEYPDTDL 126 Query: 74 QRSALELVSKAVVTNGASDTVIVDDNLFQIVAVPVRAPVIIGWVVMGFPVDKKLISDMRA 133 QRS+LELV KA NGAS+ +V D LFQIVAVP++APV I WVVMGFP+D +L+SDMR Sbjct: 127 QRSSLELVRKAGNGNGASENAVVGDRLFQIVAVPIKAPVTIAWVVMGFPIDHQLVSDMRE 186 Query: 134 LSSLQVSVLVSTRAG-QWQQDVSTLAKPESAMLMSQLPQSPAANSFIPQLSIADNDYSAR 192 LSSLQVS+LVS R G W DVSTL P ++ML+ ++ Q + L I N Y AR Sbjct: 187 LSSLQVSILVSQRGGVGWNADVSTLTAPAASMLLKEVGQPAMRAGGLIDLQIDGNQYRAR 246 Query: 193 VLVLAKGQGSQSAVAVLQRSISEAVAPYRRLQLILLSISIIGVIMAIFASAFVARRITSP 252 +L LA+ Q AVAVLQRS+SEAV PY+RLQLILLSI+++GV+ A ASA +ARRITSP Sbjct: 247 LLPLAQASNGQQAVAVLQRSVSEAVEPYQRLQLILLSITVLGVLGAAVASAVLARRITSP 306 Query: 253 LRALADIAKRLGTGDYRARIDIKGGDEIGKLADAFESMRSGIANRELEIRRLAYWDPLTD 312 LR LAD+AKRLG GDY + I+ DEIG+L +AFE+MR IA+REL+I RLAY D LT Sbjct: 307 LRDLADVAKRLGEGDYSEGLFIRRQDEIGRLGNAFETMRLEIASRELKISRLAYQDQLTG 366 Query: 313 LPNRVQFATLLNTAI-ERARTTGGQCHILMMDLDRFQHVNDVLGHSFGDNLLRQVGRRLE 371 LPNRVQFA LL AI E ART G C+ILMMDLDRFQ VNDVLGHSFGD+LLRQV RL Sbjct: 367 LPNRVQFADLLRDAIAEAARTPGAACYILMMDLDRFQQVNDVLGHSFGDDLLRQVALRLS 426 Query: 372 QQLERENDKVARLGGDEYAILLPATDQVEAVRQAKRILSSLEVPLSIDEQTVDLGAGIGI 431 QL K++RLGGDE+AILLP DQ AV QA +IL +LE PLS+ +QTVDLGAGIGI Sbjct: 427 AQLLHPGAKLSRLGGDEFAILLPGVDQEGAVEQALQILRALEQPLSLHQQTVDLGAGIGI 486 Query: 432 AGYPENGENSETLLSNAEVAMYVAKRRGSGFTVYEPSIDKSSQESLSLLSELRRALDRNE 491 +GYP +G++S+ LLS+AEVAMY+AKR+GSG +Y+P+ID+SSQESLSLLSELRRAL+R+E Sbjct: 487 SGYPAHGQSSDVLLSHAEVAMYIAKRKGSGLVIYDPAIDQSSQESLSLLSELRRALERDE 546 Query: 492 FLLYVQPKVDLASGRVVGAEALVRWMHPEKGFIGPDNFIPFAETTGFIRMLTSWMLGRTA 551 F+LY+QPK++L SG+VVGAEALVRW+HPEKGFIGPDNFIPFAETTGFIRMLT WM+ + A Sbjct: 547 FMLYLQPKLELCSGQVVGAEALVRWIHPEKGFIGPDNFIPFAETTGFIRMLTLWMMEQVA 606 Query: 552 ILCSELQRRGISLKFSVNLSTRDLLDQDLPQHFADILERNSLAPSSMCLEITESAIMDDP 611 L +LQ++G+ LKFSVNLSTRDLLDQ+LP FA IL+R+ L P+++CLEITESAIMDDP Sbjct: 607 RLSEQLQQQGVQLKFSVNLSTRDLLDQELPTAFAAILQRHRLQPAALCLEITESAIMDDP 666 Query: 612 VRAQLTLERLHAMGVELAIDDFGTGYSSLAYLKRLPVDELKIDKSFVMNMEHDADDAKIV 671 RAQ TLERL MG L+IDDFGTGYSSLAYLKRLPVDELKIDKSFVM M D DDAKIV Sbjct: 667 ARAQNTLERLSNMGFALSIDDFGTGYSSLAYLKRLPVDELKIDKSFVMKMAQDQDDAKIV 726 Query: 672 KSTIDLGHNMGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPGDRLIEWLEQWTPP 731 +ST+DLGHN+GLRVVAEG+E+ VW LLRQMGCDQ QGYFM KPMP + W EQW P Sbjct: 727 RSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPVQHFLAWREQWRAP 786 Query: 732 EVPSQSENMAI 742 ++ QSENMA+ Sbjct: 787 DMLDQSENMAV 797 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 362 bits (928), Expect = e-101, Method: Compositional matrix adjust. Identities = 196/433 (45%), Positives = 265/433 (61%), Gaps = 4/433 (0%) Query: 298 ELEIRRLAYWDPLTDLPNRVQFATLLNTAIERARTTGGQCHILMMDLDRFQHVNDVLGHS 357 E E+ A D LT L NR L AI A ++ +DLDRF+ VND LGH Sbjct: 512 EAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHK 571 Query: 358 FGDNLLRQVGRRLEQQLERENDKVARLGGDEYAILLPATDQVEA-VRQAKRILSSLEVPL 416 GD LLR V RL + RE D VARLGGDE+ ++LP D++ R +RI+ ++ P+ Sbjct: 572 AGDTLLRTVAGRLRAAV-READTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPV 630 Query: 417 SIDEQTVDLGAGIGIAGYPENGENSETLLSNAEVAMYVAKRRG-SGFTVYEPSIDKSSQE 475 ++ T+ + +G+A YP +G + ETL+ +A++AMY K+ G + F Y P++++ + E Sbjct: 631 TLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALE 690 Query: 476 SLSLLSELRRALDRNEFLLYVQPKVDLASGRVVGAEALVRWMHPEKGFIGPDNFIPFAET 535 L + +LR ALD EFLL+ QPKVDLASGRV+G+EAL+RW HP+ G + P FI AE Sbjct: 691 RLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEE 750 Query: 536 TGFIRMLTSWMLGRTAILCSELQRRGIS-LKFSVNLSTRDLLDQDLPQHFADILERNSLA 594 TG I + +W+L C + Q G + + SVNLS L +DL AD+LE L Sbjct: 751 TGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLP 810 Query: 595 PSSMCLEITESAIMDDPVRAQLTLERLHAMGVELAIDDFGTGYSSLAYLKRLPVDELKID 654 P S+ LE+TES +M D A L L +GV L+IDDFGTGYSSLAYLKR P+D LKID Sbjct: 811 PESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKID 870 Query: 655 KSFVMNMEHDADDAKIVKSTIDLGHNMGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSK 714 +SFV ++ DADDA I S I L ++ LRV+AEG+ET + LR GCD+ QGY S Sbjct: 871 RSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSP 930 Query: 715 PMPGDRLIEWLEQ 727 P+P D+ + L Q Sbjct: 931 PVPADKFEQILRQ 943 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 347 bits (891), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 197/458 (43%), Positives = 279/458 (60%), Gaps = 12/458 (2%) Query: 278 DEIGKLADAFESMRSGIANR---ELEIRRLAYWDPLTDLPNRVQFATLLNTAIERARTTG 334 DE GKL+ +E I R E E+R A D LT LPNR L A+ AR G Sbjct: 237 DEAGKLS-CYEGTVEAITERKAHEAEMRHQATHDALTGLPNRNMLHEHLQRALRVARQKG 295 Query: 335 GQCHILMMDLDRFQHVNDVLGHSFGDNLLRQVGRRLEQQLERENDKVARLGGDEYAILLP 394 + +DLD+F+ +ND LGH GD LL+ V +RL+ L RE D VAR GGDE+ ++L Sbjct: 296 ALTAVAFVDLDQFKFINDSLGHQVGDELLKTVAQRLQACL-READMVARQGGDEFVLVLQ 354 Query: 395 ATDQVEA--VRQAKRILSSLEVPLSIDEQTVDLGAGIGIAGYPENGENSETLLSNAEVAM 452 + EA +RIL+++ P ++ + A IG++ YP +G++ E+LL +A+ AM Sbjct: 355 NQTEGEAGIAEVMQRILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAM 414 Query: 453 YVAKRRG-SGFTVYEPSIDKSSQESLSLLSELRRALDRNEFLLYVQPKVDLASGRVVGAE 511 Y AK +G + F + P +D L +L LRRALD++EF LY QPK+ L GR++GAE Sbjct: 415 YRAKEQGRNNFQFFAPWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAE 474 Query: 512 ALVRWMHPEKGFIGPDNFIPFAETTGFIRMLTSWMLGRTAILCSE-LQRRGIS-LKFSVN 569 AL+RW P++G + PD FIPFAE G I + W+L RTA ++ Q+ G+ + +VN Sbjct: 475 ALIRWESPQQGMVPPDRFIPFAEEAGLIVPIGEWVL-RTACRQNQAWQQAGLPPIPVAVN 533 Query: 570 LSTRDLLDQDLPQHFADILERNSLAPSSMCLEITESAIMDDPVRAQLTLERLHAMGVELA 629 LS R L +Q LP A +L ++ LA S + LEITE+ +M D ++ TL L +G++++ Sbjct: 534 LSPRQL-NQSLPDFVAGVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQIS 592 Query: 630 IDDFGTGYSSLAYLKRLPVDELKIDKSFVMNMEHDADDAKIVKSTIDLGHNMGLRVVAEG 689 +DDFGTGYSSL+YL+R PVD LKIDKSFV ++ DAD A IVK+ I L H + LRV+AEG Sbjct: 593 VDDFGTGYSSLSYLRRFPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEG 652 Query: 690 LETLAVWNLLRQMGCDQAQGYFMSKPMPGDRLIEWLEQ 727 +E L++ CD+ QGY+ KPM W+ + Sbjct: 653 VEDEEQHAFLKENACDEVQGYYFGKPMEVAVFTAWMSE 690 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 335 bits (858), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 186/443 (41%), Positives = 249/443 (56%), Gaps = 13/443 (2%) Query: 301 IRRLAYWDPLTDLPNRVQFATLLNTAIERARTTGGQCHILMMDLDRFQHVNDVLGHSFGD 360 I +LA++D LT LPNR AI A ++ +DLDRF+ VND LGH GD Sbjct: 554 IHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNCSTVAVMFIDLDRFKQVNDSLGHQAGD 613 Query: 361 NLLRQVGRRLEQQLERENDKVARLGGDEYAILLPATDQVEAVRQAKRILSSLEVPLSIDE 420 LLR V RL+ Q R +D V RL GDE+ ++L D KRI L +P I+ Sbjct: 614 ELLRTVATRLQSQ-ARRSDIVGRLSGDEFVVVLTQYDTQHLTEVVKRIQERLAMPCHING 672 Query: 421 QTVDLGAGIGIAGYPENGENSETLLSNAEVAMYVAKRRGSG-FTVYEPSIDKSSQESLSL 479 ++ A IG+A +P+NG + ETLL A++AMY AK G F+ + ++ +QE L+L Sbjct: 673 TPLNPSASIGVAIFPDNGNDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSMAQERLAL 732 Query: 480 LSELRRALDRNEFLLYVQPKVDLASGRVVGAEALVRWMHPEKGFIGPDNFIPFAETTGFI 539 S LR AL + L QP++ L + G EAL RW HP+ G I P FIP AE G I Sbjct: 733 ESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIPIAEECGLI 792 Query: 540 RMLTSWMLGRTAILCSELQRRGISL-KFSVNLSTRDLLDQDLPQHFADILERNSLAPSSM 598 L W L S+ +RRGI + SVNLS + D DLPQ +L R +L PS + Sbjct: 793 GELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFVTGLLRRYALEPSDL 852 Query: 599 CLEITESAIMDDPVRAQLTLERLHAMGVELAIDDFGTGYSSLAYLKRLPVDELKIDKSFV 658 LEITE+ +MD T+ +HA+G+ LA+DDFGTGYSSL YL++LPV ELK+D+SFV Sbjct: 853 TLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFV 912 Query: 659 MNMEHDADDAKIVKSTIDLGHNMGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPG 718 +M D + + + I +G ++ L VVAEG+E + LL GCD AQGY S+PM Sbjct: 913 RDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGYLFSRPMSA 972 Query: 719 DRLIEWLE----------QWTPP 731 D L W+ +W PP Sbjct: 973 DALENWVRDLHAGGSPDGRWAPP 995 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 328 bits (841), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 180/430 (41%), Positives = 255/430 (59%), Gaps = 3/430 (0%) Query: 300 EIRRLAYWDPLTDLPNRVQFATLLNTAIERARTTGGQCHILMMDLDRFQHVNDVLGHSFG 359 E+ ++ D LT LPNR LN AI +A G ++ MDLD F+ +ND LGH G Sbjct: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVG 320 Query: 360 DNLLRQVGRRLEQQLERENDKVARLGGDEYAILLPATDQVEAVRQAKRILSSLEVPLSID 419 D LL +V +RL R ND VARLGGDE+ IL+ +A+ A++++ ++ P +D Sbjct: 321 DRLLVEVAQRLTSS-SRANDTVARLGGDEFVILVDLNTPEDAMGVAEKLVEAVNQPFKVD 379 Query: 420 EQTVDLGAGIGIAGYPENGENSETLLSNAEVAMYVAKRRG-SGFTVYEPSIDKSSQESLS 478 + + + A +GIA YPE+G L+ NA+ AMY KR G +G+ +EPS++ ++Q+ L Sbjct: 380 QHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQQLQ 439 Query: 479 LLSELRRALDRNEFLLYVQPKVDLASGRVVGAEALVRWMHPEKGFIGPDNFIPFAETTGF 538 L +LR AL+R +F L QPK L +G V+GAEAL+RW HP G + PD FI AE +G Sbjct: 440 WLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERSGL 499 Query: 539 IRMLTSWMLGRTAILCSELQRRG-ISLKFSVNLSTRDLLDQDLPQHFADILERNSLAPSS 597 I + +W+L E G + SVNLS D L + LER+ L P Sbjct: 500 ILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPPPC 559 Query: 598 MCLEITESAIMDDPVRAQLTLERLHAMGVELAIDDFGTGYSSLAYLKRLPVDELKIDKSF 657 + LE+TES M D + TL ++ +GVE++IDDFGTGYSSL YLKRLP +ELKID+ F Sbjct: 560 LTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDRGF 619 Query: 658 VMNMEHDADDAKIVKSTIDLGHNMGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMP 717 V + D +DA IV + + LG + LR+VAEG+ET+ + L GCD QGY M +PM Sbjct: 620 VRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRPMT 679 Query: 718 GDRLIEWLEQ 727 + ++ + + Sbjct: 680 PELFLQTMRE 689 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,054,613 Number of extensions: 81753 Number of successful extensions: 518 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 437 Number of HSP's successfully gapped: 46 Length of query: 742 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 650 Effective length of database: 1,184,250 Effective search space: 769762500 Effective search space used: 769762500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)