BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0009.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 556 e-159 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 236 2e-63 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 187 1e-48 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 151 9e-38 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 149 6e-37 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 556 bits (1432), Expect = e-159, Method: Compositional matrix adjust. Identities = 267/373 (71%), Positives = 322/373 (86%) Query: 1 VTADASFTRNLFPETTIYPPPYGGSWYNEKKAFLNLGYELDLWGKNHAALAQAVSQQKAA 60 V A+AS TRN F TTIYPPPYGG+WYNEKKA LN+GYELDLW KN AALAQA+S +KAA Sbjct: 118 VNAEASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSEKAA 177 Query: 61 EAAAQETRLVLASSIASTYNQLATEYALHDIAQRTVNQRESLQRITADRVKTGLDTQIER 120 +A+ QE RL L +SI + Y+QLA +YALHDI Q TV+QR SL++ITA+R++TGLD+QIER Sbjct: 178 QASEQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIER 237 Query: 121 NQSQGSSAEARAQLVQSEGQITLTRQQLGVLIGKGPDRGLQIAAPRMATLITPELPAQLP 180 +QS+ SSA+ARAQL QSEGQI L RQQLG L GKGPD GLQ+A P + L TP LPA+LP Sbjct: 238 DQSRTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPAELP 297 Query: 181 LNLLGRRPDIVAARWQVEAATRDIDVAKARFYPDINLSAMIGFDSLLNSNPFTASSKSIA 240 LNL+GRRPDIVAARWQVEAA+R +DVAKARFYPDINLSAMIGFD+LL+SNPFTA+SKSIA Sbjct: 298 LNLMGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIA 357 Query: 241 FGPAISLPIFEGGALRANLKGSYASYDMAVASYNQTLNEAYGDVARQITGIRSIDRQLPI 300 FGPAI+LPIFEGGALRA LKG YASY++AVA+YN+TLN+AY DVARQI I + +RQLPI Sbjct: 358 FGPAITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPI 417 Query: 301 RREALASSQRAYDLARERYRIGLTSQLVVLNAETALLGQQQALINLEASKRNQQIGLFKA 360 R EAL +++RAY LARERYR+GL SQL +L+A+T +L Q+QA++ L+A +R+QQ+ L+KA Sbjct: 418 RSEALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKA 477 Query: 361 LGGGFDAQQAGLA 373 LGGGFDAQ+ GLA Sbjct: 478 LGGGFDAQRDGLA 490 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 236 bits (603), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 150/368 (40%), Positives = 209/368 (56%), Gaps = 1/368 (0%) Query: 1 VTADASFTRNLFPETTIYPPPYGGSWYNE-KKAFLNLGYELDLWGKNHAALAQAVSQQKA 59 V+A+AS R PP + YN +A L+ YE+D WGKN AALA A S+ +A Sbjct: 127 VSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAATSELEA 186 Query: 60 AEAAAQETRLVLASSIASTYNQLATEYALHDIAQRTVNQRESLQRITADRVKTGLDTQIE 119 + A A + R+ LA+SIAS Y +LA YA D + + R + R GL+T+ Sbjct: 187 SRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLETRGS 246 Query: 120 RNQSQGSSAEARAQLVQSEGQITLTRQQLGVLIGKGPDRGLQIAAPRMATLITPELPAQL 179 Q + A A+A L ++ I L R +L L+G GPDRGLQ+ P++ LPAQL Sbjct: 247 VKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFALPAQL 306 Query: 180 PLNLLGRRPDIVAARWQVEAATRDIDVAKARFYPDINLSAMIGFDSLLNSNPFTASSKSI 239 P+ LLGRRPDIVAAR + EAA + I VA+A FYP++NL+A GF SL A S Sbjct: 307 PVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTRAGSDIG 366 Query: 240 AFGPAISLPIFEGGALRANLKGSYASYDMAVASYNQTLNEAYGDVARQITGIRSIDRQLP 299 + GPAISLPIF GG LR + + ASYD AVA+Y+Q + +A DVA +++ +L Sbjct: 367 SIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLA 426 Query: 300 IRREALASSQRAYDLARERYRIGLTSQLVVLNAETALLGQQQALINLEASKRNQQIGLFK 359 + A +++ AY + RY GL + L VLNA+ L+ + L +L + + L + Sbjct: 427 DVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVR 486 Query: 360 ALGGGFDA 367 ALGGG+ A Sbjct: 487 ALGGGYRA 494 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 187 bits (475), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 134/365 (36%), Positives = 195/365 (53%), Gaps = 2/365 (0%) Query: 4 DASFTRNLFPETTIYPPPYGGSWYNEKKAFLNLGYELDLWGKNHAALAQAVSQQKAAEAA 63 D S R + + Y + A L L Y +DLW +N A +A + A A Sbjct: 113 DVSVGRKDWSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAAD 172 Query: 64 AQETRLVLASSIASTYNQLATEYALHDIAQRTVNQRESLQRITADRVKTGLDTQIERNQS 123 A+ +L L ++ TY QLA +Y L D + + + + + + R K GL TQ+E Q+ Sbjct: 173 ARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQA 232 Query: 124 QGSSAEARAQLVQSEGQITLTRQQLGVLIGKGPDRGLQIAAPRMATLITPELPAQLPLNL 183 + E R Q+ I L R QL L+G GP + P M + LP+ LP +L Sbjct: 233 ESPLPETRRQIEALHENIALIRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADL 292 Query: 184 LGRRPDIVAARWQVEAATRDIDVAKARFYPDINLSAMIGFDSLLNS--NPFTASSKSIAF 241 +GRRPDI AARW+VEAA + I VAKA FYP+INL A IG + + +A + + Sbjct: 293 VGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTY 352 Query: 242 GPAISLPIFEGGALRANLKGSYASYDMAVASYNQTLNEAYGDVARQITGIRSIDRQLPIR 301 GPAISLPIFEGG LR L + A YD+ V YN L++A ++ Q+ ++S+ RQ Sbjct: 353 GPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQA 412 Query: 302 REALASSQRAYDLARERYRIGLTSQLVVLNAETALLGQQQALINLEASKRNQQIGLFKAL 361 ++++ ++Q+ D+A Y+ GLT L VL+A+T L QQQ + L+A + L AL Sbjct: 413 QQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTAL 472 Query: 362 GGGFD 366 GGG + Sbjct: 473 GGGIE 477 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 151 bits (381), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 104/332 (31%), Positives = 177/332 (53%), Gaps = 2/332 (0%) Query: 38 YELDLWGKNHAALAQAVSQQKAAEAAAQETRLVLASSIASTYNQLATEYALHDIAQRTVN 97 YELD +G+ A+ + + T + L + +A+ + LA++ L +AQ T Sbjct: 157 YELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYA 216 Query: 98 QRESLQRITADRVKTGLDTQIERNQSQGSSAEARAQLVQSEGQITLTRQQLGVLIGKGPD 157 + ++ G + ++ + + ARA + + Q+ L +L+G D Sbjct: 217 SQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNALRLLVGADID 276 Query: 158 RGLQIAA-PRMATLITPELPAQLPLNLLGRRPDIVAARWQVEAATRDIDVAKARFYPDIN 216 L A P A+++ P PA LP +L RRPD++AA +++A DI A+A F+P I+ Sbjct: 277 AALLPAGLPDTASVL-PATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSIS 335 Query: 217 LSAMIGFDSLLNSNPFTASSKSIAFGPAISLPIFEGGALRANLKGSYASYDMAVASYNQT 276 L+A G S F A S S F P+I+LPIF G+LRA+L + + D+ VA+Y + Sbjct: 336 LTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKA 395 Query: 277 LNEAYGDVARQITGIRSIDRQLPIRREALASSQRAYDLARERYRIGLTSQLVVLNAETAL 336 + A+ +VA + ++D++L + +A A+S++A+ L+ RYR G+ S L L A+ L Sbjct: 396 IQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTL 455 Query: 337 LGQQQALINLEASKRNQQIGLFKALGGGFDAQ 368 QQ LI+L ++++ +I L+K LGGG+ Q Sbjct: 456 YSAQQTLISLRLTEQSNRITLYKVLGGGWSEQ 487 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 149 bits (375), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 108/338 (31%), Positives = 173/338 (51%), Gaps = 16/338 (4%) Query: 38 YELDLWGKNHAALAQAVSQQKAAEAAAQETRLVLASSIASTYNQLATEYALHDIAQRTVN 97 YELDL+G+ + QA+ + A + T++ L + +A+ + LA++ +AQ T+ Sbjct: 150 YELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLE 209 Query: 98 QRESLQRITADRVKTGLDTQIERNQSQGSSAEARAQLVQSEGQITLTRQQLGVLIGKG-- 155 + S +T + G + + Q+Q S AR + Q+ R L +L+GK Sbjct: 210 SQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGKDLP 269 Query: 156 --------PDRGLQIAAPRMATLITPELPAQLPLNLLGRRPDIVAARWQVEAATRDIDVA 207 PD L A+P +AT+ P LP +LL RR DIV A + AA +I A Sbjct: 270 TALLPQGLPDTRLAAASP-LATI-----PPGLPSDLLQRRADIVQAERDLRAANANIGAA 323 Query: 208 KARFYPDINLSAMIGFDSLLNSNPFTASSKSIAFGPAISLPIFEGGALRANLKGSYASYD 267 +A FYP I L+ G S + F S S +F P ISLPIF+GGA +ANL + S D Sbjct: 324 RAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATVSRD 383 Query: 268 MAVASYNQTLNEAYGDVARQITGIRSIDRQLPIRREALASSQRAYDLARERYRIGLTSQL 327 ++VA Y + + A+ +V+ + ++ RQL + + +S A+ L++ RY G+ S L Sbjct: 384 ISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQARYHSGVDSYL 443 Query: 328 VVLNAETALLGQQQALINLEASKRNQQIGLFKALGGGF 365 VL+++ L Q+ LI + S+ + +K LGGG+ Sbjct: 444 TVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGW 481 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,058,983 Number of extensions: 40492 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 18 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)