BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0007.p (430 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 475 e-135 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 240 2e-64 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 56 7e-09 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 475 bits (1223), Expect = e-135, Method: Compositional matrix adjust. Identities = 264/301 (87%), Positives = 288/301 (95%) Query: 19 NGNGNGKRKRQLLLLTLVLIIVAIGLFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTV 78 N NGNGKRKRQL+LLTLVL+++A+ FLYWFLHARFFEETDDAYVGGNVVQISAQVGGTV Sbjct: 13 NNNGNGKRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTV 72 Query: 79 TAIKADDTQVVKAGQQLVSLDAADTKLALAQAEAALAQAVRQTRQTFLNNDTLAANVTAA 138 A+KADDTQVVKAGQQLV+LDAADT+LAL QA+AALAQAVRQTRQ FLNNDTLAANV AA Sbjct: 73 VAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAA 132 Query: 139 ETNLARTRDDLQRRQAGIASGAVSQEDLAHAQDAVKNANASLEQARASLAANRALTDHTS 198 ++NLAR R+DLQRRQAG++SGAVSQED++HA+DAVK+A A+L+QARA+ AANRALTDHTS Sbjct: 133 DSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQARAAAAANRALTDHTS 192 Query: 199 VTEHPNVLQAATQVRNAYLNYARVNIVAPVSGFVSKRSVQLGQRIAPGNPLMAIVPLEQI 258 VTEHPNVLQAAT VRNAYLNYARVNIVAPVSGFVSKRSVQ+GQRIA GNPLMAIVPLEQI Sbjct: 193 VTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQI 252 Query: 259 WIDANFKEGQLKHIRIGQPVEVIADVYGSSVKYKGTVVGFSAGTGGAFSLLPAQNATGNW 318 WIDANFKE QL+HIRIGQPVEVIADVYGSSVKYKGTV+GFSAGTGGAFSLLPAQNATGNW Sbjct: 253 WIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNW 312 Query: 319 I 319 I Sbjct: 313 I 313 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 240 bits (612), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 135/323 (41%), Positives = 193/323 (59%), Gaps = 28/323 (8%) Query: 25 KRKRQLLLLTLVLIIVAIGLFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVTAIKAD 84 KRK+ + V+ I AIG YW+L + ETD+AY + ++ + G V A+ Sbjct: 31 KRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTATEIATVTPAINGIVAAVDVV 90 Query: 85 DTQVVKAGQQLVSLDAADTKLALAQAEAALAQAVRQTRQTFLNNDTLAANVTA------- 137 DTQ VK G LV +D AD +LA+ QA A L + R+ + F N+ LAA V A Sbjct: 91 DTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQKR 150 Query: 138 -------AETNLARTRDDLQRRQAGIASGAVSQEDLAHAQDAVKNANASLEQARASLAAN 190 A+ +L R DLQRR+A SG+VS E+L++A+ A+ A A+L+ A A+ + Sbjct: 151 ASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 210 Query: 191 RA--------------LTDHTSVTEHPNVLQAATQVRNAYLNYARVNIVAPVSGFVSKRS 236 RA LT +T+V ++P V+ A ++ A L+ R + APV G +++R Sbjct: 211 RANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKLDLERTVLRAPVDGVIARRQ 270 Query: 237 VQLGQRIAPGNPLMAIVPLEQIWIDANFKEGQLKHIRIGQPVEVIADVYGSSVKYKGTVV 296 VQ+GQR+ G L+++VPL+Q+ +DANFKEGQL +RIGQPV + AD+YG SV+Y G V Sbjct: 271 VQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVT 330 Query: 297 GFSAGTGGAFSLLPAQNATGNWI 319 G S GTG AF+++PAQNATGNWI Sbjct: 331 GLSGGTGSAFAVIPAQNATGNWI 353 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 55.8 bits (133), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 69/254 (27%), Positives = 112/254 (44%), Gaps = 35/254 (13%) Query: 31 LLLTLVLIIVAIGLFLYWFLHARFFEE--TDDAYVGGNVVQISAQVGGTVTAIKADDTQV 88 +L+T + ++AI L W L + T D V VV I+ V G V A+ D Q+ Sbjct: 9 VLITTAIFLLAI--LLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALPVRDNQL 66 Query: 89 VKAGQQLVSLDAADTKLALAQAEAALAQAVRQTRQTFLNNDTLAANVTAAETNLARTRDD 148 VK G L+ +D A LAL QAEAA VTA + L R + Sbjct: 67 VKKGDLLMEIDPARYTLALQQAEAA---------------------VTARKAELDVRRAE 105 Query: 149 LQRRQAGI------ASGAVSQEDLAHAQDAVKNANASLEQARASLAANRALTDHTSVTEH 202 ++ R+A + A + L +++A ++A + + ++A L A +A Sbjct: 106 VEARRADLNMRRAQARRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQYQ 165 Query: 203 PNVLQAATQVRNAYLNYARVNIVAPVSGFVSKRSVQLGQRIAPGNPLMAIVPLEQIWIDA 262 + Q T A LN R + +PV G+++ +V+ G A G +A++ W+ A Sbjct: 166 AALSQRDT----ARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYA 221 Query: 263 NFKEGQLKHIRIGQ 276 F+E +L + IG Sbjct: 222 YFEETKLPLLSIGD 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.128 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 977,752 Number of extensions: 31607 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 7 Length of query: 430 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 342 Effective length of database: 1,203,190 Effective search space: 411490980 Effective search space used: 411490980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)