BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0006.p (521 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 875 0.0 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 419 e-118 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 214 2e-56 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 146 5e-36 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 132 8e-32 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 875 bits (2262), Expect = 0.0, Method: Compositional matrix adjust. Identities = 449/524 (85%), Positives = 491/524 (93%), Gaps = 3/524 (0%) Query: 1 MASSP---QTYTPPPPLTGGKLVLGTLCVSLAVFMNVLDSSIANVAIPTISGNLGVSIDE 57 MA+SP Q YTPPPPLTG LVLGTL VSLAVFMNVLDSSIANVAIPTISGNLGVS+DE Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 58 GTWVITSFAAANAISIPLTGWLTQRIGAVRLFVSSVLLFVLASWLCGLAPTLPILLGARV 117 GTWVITSFAAANAISIPLTGWLTQR+GAVRLFV+SVLLFVLASWLCGLAPTLPILL ARV Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 Query: 118 LQGLVAGPMVPLSQSLLLGAYPKSKASFALALWGMTAVVAPVAGPALGGWITDAYTWPWI 177 LQGLVAGPMVPLSQSLLLGAYPKSK+SFALALWGMTAVVAPVAGPALGGWITD+YTWPWI Sbjct: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 Query: 178 FYINIPVGLLAAGMTMAIYKDRESPTHKLPIDTIGLLLLITWVAAFQIMLDKGKDLDWFA 237 FYINIPVGL+AAG+T+AIY+ RE+PTHKLPID IGL LL+TWVAAFQIMLDKGKDLDWFA Sbjct: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 Query: 238 SPTIVTLGIIAAVAFAYFVIWELNNDHPVVDLRLFGRRNFFGGTVAISIGYGVFFGNLVL 297 SPTIVTLGIIA +AFAYFV+WELNNDHPVVDLRLFGRRNF GGT+AISIGYGVFFGNLV+ Sbjct: 241 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 Query: 298 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGKFLPRMDARKVATASFVGFACVFFM 357 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIG+FLP +DARKVATASF+GFA VF++ Sbjct: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 Query: 358 RSRYNLEVDQWTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 417 RSRYNL+VDQ TVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA Sbjct: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 Query: 418 IGTSVATTAWNNRTIVHHAQLTEHATPYSTALQQMFSNLTGNFGMSTEQATGLIERNLNA 477 +GTS++TTAWNNR+I+HHAQLTEHATPYS LQ+ +NL+ G++ EQ G++ER + Sbjct: 421 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 480 Query: 478 QAAMIGINDIFWLSGVIFLVIIPVIWIIKPAKGNAGAEAAAAAH 521 QAAM+GINDIF++S VIF+VIIP+IWIIKP+KG AGA A+AAH Sbjct: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSKGGAGAAEASAAH 524 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 221/497 (44%), Positives = 313/497 (62%), Gaps = 6/497 (1%) Query: 14 LTGGKLVLGTLCVSLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISI 73 L+GG+LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+VITS+A A AI++ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 74 PLTGWLTQRIGAVRLFVSSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSL 133 PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+ RVLQGL GP++PLSQ+L Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 134 LLGAYPKSKASFALALWGMTAVVAPVAGPALGGWITDAYTWPWIFYINIPVGLLAAGMTM 193 LL +PK KA A+ LW MT +VAP+AGP LGG + D Y+WP+IF+IN+PV L+ + Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 194 AIYKDRESPTHKLPIDTIGLLLLITWVAAFQIMLDKGKDLDWFASPTIVTLGIIAAVAFA 253 + K E+ KLPID +GL+L++ WVAA Q+MLD+GK+ DWFAS IV L + A V F Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 254 YFVIWELNNDHPVVDLRLFGRRNFFGGTVAISIGYGVFFGNLVLLPQWLQQYLGYRSIDS 313 F+IWEL +P+VDLR+F R F I + +G FFG++VL P WLQ Y+GY + S Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 314 GLSTAPIGIFAVILMPLIGKFLPRMDARKVATASFVGFACVFFMRSRYNLEVDQWTVILP 373 G +A GI AV+ P++ K +DARK+ + F+R+ ++ + + P Sbjct: 326 GCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWP 385 Query: 374 TLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAIGTSVATTAWNNRTIV 433 LQGI +FFVPLT L LS + E +AAGL SF R GAI TS+ T+W N Sbjct: 386 LFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 Query: 434 HHAQLTEHATPYSTALQQMFSNLTGNFGMSTEQATGLIERNLNAQAAMIGINDIFWLSGV 493 HA+L+ A+ M ++ GMS EQAT + + + QA M+ N IF ++ V Sbjct: 446 FHAELSGLVDRGGDAIHTMMAS-----GMSAEQATASLAQTVQNQAVMLSTNHIFMMAAV 500 Query: 494 IFLVIIPVIWII-KPAK 509 F + +W+ KP + Sbjct: 501 SFSLAALAVWLAPKPTR 517 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 214 bits (544), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 144/497 (28%), Positives = 250/497 (50%), Gaps = 7/497 (1%) Query: 27 SLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRIGAV 86 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + +G Sbjct: 23 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 82 Query: 87 RLFVSSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKASFA 146 L +++ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 83 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 142 Query: 147 LALWGMTAVVAPVAGPALGGWITDAYTWPWIFYINIPVG-LLAAGMTMAIYKDRESPTHK 205 A++G TA++ PV GP +GGW+T+ +W + F+IN+PVG L + +AI +R Sbjct: 143 NAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQL 202 Query: 206 LPIDTIGLLLLITWVAAFQIMLDKGKDLDWFASPTIVTLGIIAAVAFAYFVIWELNNDHP 265 D G+ L + ++L++G+ WF+SP I L + + F I ++ HP Sbjct: 203 AEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHP 262 Query: 266 VVDLRLFGRRNFFGGTVAISIGYGV-FFGNLVLLPQWLQQYLGYRSIDSGLSTAPIGIFA 324 V+ LRL R FG V +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 263 VIQLRLLRDRQ-FGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPM 321 Query: 325 VILMPLIGKFLPRMDARKVATASFVGFACVFFMRSRYNLEVDQWTVILPTLLQGIPTAMF 384 +++MP + +D R F+ A F+ + + + + L++G+ T + Sbjct: 322 ILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLMRGVGTVLA 381 Query: 385 FVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAIGTSVATTAWNNRTIVHHAQLTEHATP 444 + L + +PP + A+GL S R G++ + + R +H +L E Sbjct: 382 MLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESLNA 441 Query: 445 YSTALQQMFSNLTGNFGMSTEQATGLIERNLNAQAAMIGINDIFWLSGVIFLVIIPVIWI 504 S +Q + G+ + A L+ + +QA + D+FW+ GV L + P++ Sbjct: 442 NSDLVQSSMTA-QGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLF 500 Query: 505 IKPAKGNAGAEAAAAAH 521 ++P +A A A+H Sbjct: 501 LRPLPTHA---APVASH 514 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 146 bits (368), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 127/420 (30%), Positives = 193/420 (45%), Gaps = 37/420 (8%) Query: 26 VSLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRIGA 85 V+ FM LD++I N A+P ++ +LG + V+ ++ A P +GWL R G Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 86 VRLFVSSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKASF 145 R++ +++LLF L S LC +A +LP+LL ARV+QGL ++P+ + +L A P ++ Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 146 ALALWGMTAVVAPVAGPALGGWITDAYTWPWIFYINIPVGLLAAGMTMAIYKDRESPTHK 205 ALA + + PV GP LGGW+ ++ +W WIF IN+PVG+L G + D +S Sbjct: 178 ALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPD-DSLAGT 236 Query: 206 LPIDTIGLLLLITWVAAFQIMLDKGKDLDWFASPTIVTLGIIAAVAFAYFVIWELNNDH- 264 D +G +LL + AF + LD D + T ++ + A W + H Sbjct: 237 PRFDLLGCVLLSACMVAFSLALDVPFD----SHRTGWSIALFVVAALTVLGYWRHASVHP 292 Query: 265 -PVVDLRLFGRRNFFGGTVAISIGYGVFFGNLVL----------LPQWLQQYLGYRSIDS 313 P+ L LFG NF SIG GNLV LP LQ LGY + + Sbjct: 293 RPLFRLGLFGEPNF-------SIGLA---GNLVCRIGSSAVPFLLPLLLQVRLGYSPLHA 342 Query: 314 GLSTAPIGIFAVILMPLIGKFLPRM--DARKVATASFVGFACVFFMRSRYNLEVDQW--- 368 G+ P I I P I + R D + + VG A + F L W Sbjct: 343 GMVLLPAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSF-----ALITPDWPLT 397 Query: 369 TVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAIGTSVATTAWN 428 I+ + G +M F + + L GL + L S V++ +G ++ T N Sbjct: 398 AAIVQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 132 bits (331), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 93/315 (29%), Positives = 157/315 (49%), Gaps = 14/315 (4%) Query: 35 LDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRIGAVRLFVSSVL 94 +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R G++ +F S++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 95 LFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKASFALALWGMTA 154 +F+L S LCG++ TLP + AR LQG+ MVP+ + ++L + K AL+ + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 155 VVAPVAGPALGGWITDAYTWPWIFYINIPVGLLAAGMTMAIYKDRESPTHKLPIDTIGLL 214 + PV GP LGG+IT + W WIF+INIP+ +L + D ++ P+D G L Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKA-EQVAPLDLKGFL 179 Query: 215 LLITWVAAFQIMLDKGKDLDWFASPTIVTLGIIAAVAFAYFVIWELNN-DHPVVDLRLFG 273 L + + +M+ S + ++A + F +W P++DL L Sbjct: 180 L--SALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLK 237 Query: 274 ----RRNFFGGTVAISIGYGVFFGNL-VLLPQWLQQYLGYRSIDSGLSTAPIGIFAVILM 328 R FGG+ Y V G + LLP LQ GY SG+ T A+ + Sbjct: 238 IPTLRAGVFGGSF-----YRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMK 292 Query: 329 PLIGKFLPRMDARKV 343 + +FL R R+V Sbjct: 293 TIAARFLRRYGFRRV 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,516,782 Number of extensions: 61778 Number of successful extensions: 404 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 392 Number of HSP's successfully gapped: 15 Length of query: 521 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 431 Effective length of database: 1,193,720 Effective search space: 514493320 Effective search space used: 514493320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)