BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0003.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 744 0.0 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 234 1e-62 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 100 2e-22 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 99 7e-22 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 71 2e-13 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 744 bits (1922), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/368 (98%), Positives = 365/368 (99%) Query: 22 MKTKAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL 81 MKT+AAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL Sbjct: 1 MKTRAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL 60 Query: 82 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRSTQGRGLMPD 141 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIR+TQGRGLMPD Sbjct: 61 GHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRATQGRGLMPD 120 Query: 142 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGCGVTTGVGAVLF 201 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIR DAPFDKVCYIGCGVTTGVGAVLF Sbjct: 121 ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVTTGVGAVLF 180 Query: 202 TAKVEAGANVVVFGLGGIGLNVIQAARMVGANKIIGVDLNPGREAMARKFGMTDFINPKD 261 TAKVEAGANVVVFGLGGIGLNVIQAA+MVGANKIIGVDLNP REAMARKFGMTDFINPKD Sbjct: 181 TAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGMTDFINPKD 240 Query: 262 VSNVVDAIIGLTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 321 V NVVDAIIG+TDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF Sbjct: 241 VPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 300 Query: 322 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE 381 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE Sbjct: 301 QLVTGRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSGE 360 Query: 382 SIRSVVEF 389 SIRSVVEF Sbjct: 361 SIRSVVEF 368 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 234 bits (596), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 134/377 (35%), Positives = 200/377 (53%), Gaps = 12/377 (3%) Query: 22 MKTKAAV--------AWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP 73 MK +AAV + +PL IEE++L P GEVLV +KA G+CH+D ++G P Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 74 EGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRST 133 + P LGHE + VV+VG GV L+ D V+ ++ P C C C+ + LC+ +T Sbjct: 61 RPV-PMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 Query: 134 QGRGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGCGVT 193 G + R +P+ H++G S F+++ VV + KI D + GC V Sbjct: 120 NTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 Query: 194 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAARMVGANKIIGVDLNPGREAMARKFGM 253 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ + A+AR G Sbjct: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGA 239 Query: 254 TDFINPKDVSNVVDAIIGLTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAG 313 T +N +D + V+ + LT GG DY+FE G+ M + +G G ++ G+ A G Sbjct: 240 TATVNARD-PDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPG 297 Query: 314 QEISTRPFQLVT-GRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINE 372 Q + + LV R KGS G D+P+ + + GKL +D L+ +L L IN Sbjct: 298 QHWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINR 357 Query: 373 GFDLMKSGESIRSVVEF 389 GFD + GE +R ++ F Sbjct: 358 GFDRLHGGEGLRDLIVF 374 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 100/361 (27%), Positives = 169/361 (46%), Gaps = 39/361 (10%) Query: 25 KAAVAWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP-EGIFPAILGH 83 KAAV + GKPL+IE+V + P G++LV+ +A+G+CHTD + G P + P I GH Sbjct: 6 KAAVVREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPGH 65 Query: 84 EGAGVVVDVGPGVKSLKKDDHV-IPLYTPECRECKFCLSQKTNLCQAIRSTQGRGLMPDA 142 EG G V VG GVK +K+ D V +P C C C + LC ++T Sbjct: 66 EGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNTG-------- 117 Query: 143 TSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGC-GVTTGVGAVLF 201 +S++G +F+ Y + + + ++ F + C GVT G L Sbjct: 118 ---YSVNG----------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKG--LK 162 Query: 202 TAKVEAGANVVVFGLGGIGLNVIQAARMVGANKIIGVDLNPGREAMARKFGMTDFINPKD 261 +V G VV+ G+GG+G +Q A+ +G + ++ D++ + A+A+K G ++ ++ Sbjct: 163 ETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRN 221 Query: 262 VSNVVDAIIGLTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPF 321 + V + + GGA + + M Q+ + G ++G+ +IS F Sbjct: 222 HNAVAE--VQRIIGGAHGALVTAVSPKAMEQAFGFL-RARGTMALVGLPPG--DISLPVF 276 Query: 322 QLVTGRVW-KGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMKSG 380 V R+ +GS G R D+ + + + EGK+ L+ IN F M+ G Sbjct: 277 NTVLKRITVRGSIVG---TRQDLEESLVFAAEGKVAAHFTWDK---LDNINAIFARMEEG 330 Query: 381 E 381 + Sbjct: 331 K 331 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 98.6 bits (244), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 108/370 (29%), Positives = 164/370 (44%), Gaps = 39/370 (10%) Query: 47 KAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVI 106 +A +V++++ AT IC +D + G P ILGHE GVVV+ GP +K LKK D V+ Sbjct: 24 EADDVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVV 83 Query: 107 PLYTPECRECKFCLSQKTNLCQAIRSTQGRGLMPDATSRFSLDGKPLFHYMGT-----ST 161 + C +C FC C+ + RG + +A S G +F Y Sbjct: 84 VPFVIACGQCFFCDQHLYAACET--TNPDRGALMNAKGIRS--GAAMFGYSHLYGGMPGG 139 Query: 162 FSNYIVVPEIALAKIREDAPF--DKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGI 219 + Y+ VP+ + IR D+ ++V ++ + TG AV A V G++V ++G G + Sbjct: 140 QAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVK-NAGVREGSSVAIYGAGPV 198 Query: 220 GLNVIQAARMVGANKIIGVDLNPGREAMARKFGMTDFINPK-DVSNVVDAIIGLTDG-GA 277 G +ARM+GA +I VD + R A++ D I D + + II T G G Sbjct: 199 GQMAAASARMLGAERIFMVDHHAYRLRFAQE--QYDVIPVNFDQVDPAEYIIANTAGRGV 256 Query: 278 D-----YSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAA--GQEISTRPFQLVTGRVWK 330 D FE G+ + GQ++ +A+ G IS V Sbjct: 257 DGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPG---VYAGFIH 313 Query: 331 GSAFGG--------ARGRTDV----PKIVDWYMEGKLNIDDLITHKLPLERINEGFDLMK 378 G FG G+T V P+++ EG+L D +ITH+LPLE G+ + Sbjct: 314 GFLFGDIFEKGVTVKTGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGYQIFN 373 Query: 379 SG-ESIRSVV 387 E R VV Sbjct: 374 DKLEDCRKVV 383 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 89/346 (25%), Positives = 141/346 (40%), Gaps = 33/346 (9%) Query: 36 LTIEEVELGGPKAGEVLVEIKATGICHTD--YYTLSGADPEGIF-PAILGHEGAGVVVDV 92 L I+ ++ G + ++ V++KA GIC +D YY G I P +LGHE +G + +V Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 93 GPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRSTQGRGLMPDATSRFSLDGKP 152 G V + + + C CK+C K N C +R P Sbjct: 73 GTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTP------------ 120 Query: 153 LFHYMGTSTFSNYIVVPEIALAKIREDAPFDKVCYIGCGVTTGVGAVLFTAKVEAGANVV 212 H G F I++ E + A + D D + ++ + AV + G V+ Sbjct: 121 --HVQG--AFRQEIII-ERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPL-VGKRVL 174 Query: 213 VFGLGGIGLNVIQAARMVGANKIIGVDLNPGREAMARKFGMTDFINPKDVSNVVDAIIGL 272 V G G IG V+ AAR GA +++ D+ A + G IN + + A Sbjct: 175 VTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAA- 233 Query: 273 TDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAGQEISTRPFQLVTGR--VWK 330 G D FE GN + +LE Q+II+ V G P L+ G+ W+ Sbjct: 234 DKGTFDVLFEASGNERALVGALEALRP---QAIIVQVGLGGD--IQFPANLLVGKELEWR 288 Query: 331 GSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINEGFDL 376 G AF R + V+ +++ LIT P+ + E F++ Sbjct: 289 G-AF---RFHEEFAMAVELMNRKLVDVKPLITATYPIAQAVEAFEI 330 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,226,243 Number of extensions: 53121 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 6 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)