BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0047.p (133 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 158 2e-40 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 64 3e-12 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 158 bits (399), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 77/119 (64%), Positives = 91/119 (76%) Query: 11 QADAAGLHILVVEDNLDSLQLVCELLAVLGHRAEGVGSAEEALAELEQHDFDVLFTDVSL 70 Q DA GL +LVVEDN+DS QLVCELL LGH+A GV S EEAL LE+ FDVLFTDV+L Sbjct: 1158 QEDAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNL 1217 Query: 71 PGMSGLELAKRTSEKTPALKIIFASGYGAQISKNIGPGTFSLPKPYDIDQLQDILDNIG 129 PGMSG+ELAK + P L +IFASGYG +++N+G +LPKPYDIDQLQ+ILD I Sbjct: 1218 PGMSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAHALPKPYDIDQLQEILDKIA 1276 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 64.3 bits (155), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 40/108 (37%), Positives = 62/108 (57%), Gaps = 4/108 (3%) Query: 18 HILVVEDNLDSLQLVCELLAVLGHRAEGVGSAEEALAELEQ-HDFDVLFTDVSLPG-MSG 75 HILVV+D L+ +L +L LG+R V S EAL + D+LFTD+ +PG M+G Sbjct: 430 HILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTDLIMPGSMNG 489 Query: 76 LELAKRTSEKTPALKIIFASGYGAQ-ISKNIGPGTFS-LPKPYDIDQL 121 + LA+ P +K++ +GY ++ + ++ G F L KPY D+L Sbjct: 490 VVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDEL 537 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 386,169 Number of extensions: 14734 Number of successful extensions: 137 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 5 Length of query: 133 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 58 Effective length of database: 1,264,745 Effective search space: 73355210 Effective search space used: 73355210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 125 (52.8 bits)