BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0038.p (1119 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311640|ref|YP_003775732.1| alpha-amylase [Herbaspirillum s... 1451 0.0 >gi|300311640|ref|YP_003775732.1| alpha-amylase [Herbaspirillum seropedicae SmR1] Length = 1132 Score = 1451 bits (3757), Expect = 0.0, Method: Compositional matrix adjust. Identities = 738/1161 (63%), Positives = 863/1161 (74%), Gaps = 71/1161 (6%) Query: 1 LENHAAAAFSPRIYCIDPTLVGGGGWSDLFARCAGMGFDHVLLCGDGLIAGLFAGEDSHV 60 +EN FSPRIYC+DP+LV W DL RCA MGFDHVLLCGD L+ FA Sbjct: 1 MENLPLTDFSPRIYCVDPSLVASPDWDDLLGRCAAMGFDHVLLCGD-LVDASFA------ 53 Query: 61 DNVESPIADYGMASLDQLEELAHLATEHRLRLLLDLSLTSVHGSDQLLRSHPDWFAVAGA 120 E+A L HRLRLL DLSLT + D +L SH DWFA + Sbjct: 54 -------------------EVAKLCKGHRLRLLADLSLTRFYAGDPVLTSHADWFAPQSS 94 Query: 121 A----SDASAHGDHLPDPRRP-------------QPRFHAPEIAEALLECWHMRLAMLID 163 A S D LPDPR P + RF P A+A+L+ W RL L+D Sbjct: 95 AAVGLSSQDLASDPLPDPRYPSVMDERDDELVTRRARFDDPVAADAMLDWWRARLMTLVD 154 Query: 164 AGVAGFLCHDPAGLPGNFWCRLVERTREQRSCCQFLAWTPGCSHPQLESLADCGFDAVFS 223 AGVAGF C +PA +P +FW RL+ R+Q++ C+FLAWTPGC+H QL+ LA GFDAVFS Sbjct: 155 AGVAGFRCQEPASIPADFWRRLIAGIRQQQADCRFLAWTPGCTHDQLDGLAGIGFDAVFS 214 Query: 224 SGAWWDFRAPWYFREHEKLVAIAPVLGFPEDPFGPRAARVHGWHDKETRRLGAIRALRFA 283 SGAWWDFR PWYFRE+ KL AIAPVL FPEDP GPR ARVHG+ D +TRRLGA+RAL+FA Sbjct: 215 SGAWWDFRDPWYFREYRKLAAIAPVLAFPEDPSGPRLARVHGFRDPQTRRLGALRALQFA 274 Query: 284 ALSGSGWLMPMGFEFGLTEALGIAHNAAASTRLTTPQQFAQACAESDFNLDAEVRAANQA 343 LS GW+MPMGFEFGLT AL +AH A ++TRL+ P+ F +ACA++D + E+ AN+ Sbjct: 275 TLSAEGWMMPMGFEFGLTAALRVAHRAGSATRLSDPEAFKRACADADLDFTREITEANRT 334 Query: 344 MS--------AGGFRLRAVSAPEAPVLLLAR---DVGVHQLIAINPRLGQVARLADGDWQ 392 ++ + RL +SAP++P LL+ R +V LIA+NP L + R+ D WQ Sbjct: 335 LAQRRQSPPKSLALRLTPLSAPDSPWLLMERHGVNVEDGLLIAVNPALDRPVRITDDGWQ 394 Query: 393 QRLDGRIVDWPPEREFILQPGEIALIELQTPPPVAADLSRRKKPVAEKVRTGRIAIEQVS 452 QRL + W E +L+PG + ++ L+ V+ +L + P +R+ RIAIE+VS Sbjct: 395 QRLQESVAPWQ-EDGLVLEPGAVKVLPLKPLQGVSGELDQ-ATPALGDMRSRRIAIERVS 452 Query: 453 PAVDGGRFAVKRTVGQSFTISADIFMDGHDHLAAAVLVRAADEKQWQRIPLQHVGNDRWQ 512 PAVD G FA KR VG+ F I+ADIFMDGHDHLAA VLVRAADE QW+RIP+ NDRWQ Sbjct: 453 PAVDDGAFAAKRIVGEHFVIAADIFMDGHDHLAAEVLVRAADEAQWRRIPMHPDVNDRWQ 512 Query: 513 ASLSLPRLGRHYFCIEAWSDVFETYRDGLKKKSAAGLNVDLELEEGRLLVANLAEQALEN 572 A +SL RLGRHYFCIEAWSDVF+T+RDGL+KK AG +V LE+EEGRLL+A L ++A+E Sbjct: 513 ARVSLTRLGRHYFCIEAWSDVFDTFRDGLQKKLMAGQDVSLEMEEGRLLIARLVQRAVEQ 572 Query: 573 GIDGAVLEGGGDLLKSL-----------SGKNGNHNGNRLAVLLADDTARQIDRLTELAG 621 ++ +VLE LLK+L +GK + + RLA L A++T + +++LT LAG Sbjct: 573 ELEPSVLESSRALLKALGGPPSKTRRSAAGKQADTD-KRLAFLCAEETRQLVEQLTGLAG 631 Query: 622 ARAFLMRSATEYPLETERPAATFSSWYELFPRSQSGDAAVHGTFNDVIARLPAIRAMGFD 681 RAFL R+ EYPLE ER +A FSSWYELFPRSQSG A VHG F+DVIARLPAIRAMGFD Sbjct: 632 ERAFLSRTDAEYPLEAERRSAAFSSWYELFPRSQSGSADVHGNFDDVIARLPAIRAMGFD 691 Query: 682 TLYFPPIHPIGKKNRKGKNNSLTPGDDDPGSPYAIGSEEGGHDAIHPQLGTLDDFLRLRD 741 TLYFPPIHPIGKK+RKGKNNS+T G DDPGSPYAIGSEEGGHDAIHPQLGT +DF RLRD Sbjct: 692 TLYFPPIHPIGKKHRKGKNNSVTAGPDDPGSPYAIGSEEGGHDAIHPQLGTFEDFARLRD 751 Query: 742 AAAAHGLELALDFAIQCAPDHPWLKEHPGWFSWRPDGSMRYAENPPKKYQDIVNVDFYAE 801 AAAAHGLELALDFAIQC+PDHPWLK HPGWF+WRPDGSMRYAENPPKKYQDIVNVDFYAE Sbjct: 752 AAAAHGLELALDFAIQCSPDHPWLKTHPGWFAWRPDGSMRYAENPPKKYQDIVNVDFYAE 811 Query: 802 DAIPDLWIALRDIVLYWVQQGVNVFRVDNPHTKPFPFWEWMIAAVRSRYPQVIFLSEAFT 861 DAIP+LW+ALRDIV++WVQQGV+VFRVDNPHTKPFPFWEWMIA+VRSRYPQVIFLSEAFT Sbjct: 812 DAIPELWLALRDIVVFWVQQGVSVFRVDNPHTKPFPFWEWMIASVRSRYPQVIFLSEAFT 871 Query: 862 RPKPMYRLAKVGFSQSYTYFTWRHDKQEFIDYLEELTGSPDGDTRPRDFFRPHFFVNTPD 921 RPKPMYRLAKVGFSQSYTYFTWRH KQE IDYL ELT +PRDFFRPHFFVNTPD Sbjct: 872 RPKPMYRLAKVGFSQSYTYFTWRHGKQELIDYLTELTTDTGHAAQPRDFFRPHFFVNTPD 931 Query: 922 INPYFLQRSGRAGFVIRAALAATLSGLWGVYSGFELCEAQPVPGKEEYLDSEKYQVRAWD 981 INPYFLQRSGRAGF IRAALAATLSGLWGVYSGFEL EA VPGKEEYLDSEKY++R+WD Sbjct: 932 INPYFLQRSGRAGFQIRAALAATLSGLWGVYSGFELGEANAVPGKEEYLDSEKYEIRSWD 991 Query: 982 WQRPGNIIEDIARLNRIRRENPALQTHLGVHFLPSSNDKVLYFVKSTAPAYSVVDAVDDR 1041 WQRPGNIIEDI RLNRIR ENPALQTHLGV FLP SND++LYFVKST A S AV+ Sbjct: 992 WQRPGNIIEDITRLNRIRTENPALQTHLGVRFLPVSNDQILYFVKSTPAAPSGRAAVEGG 1051 Query: 1042 LRFGDNVILVAINLDPFNEQSGDIDIPLYGFGL---GDDSRVQVEDLLTDRRFNWTGRRH 1098 LRFGDN ILVAINLDPF Q G+I++PL GFGL + + ++V DLL + RF W GR Sbjct: 1052 LRFGDNTILVAINLDPFQSQQGEIELPLAGFGLPEGAEPAHLEVVDLLGEDRFKWYGRHQ 1111 Query: 1099 QLSLNPDKNPYGIWRLRAMET 1119 ++L+P + PY IWR+RA ET Sbjct: 1112 SITLDPRERPYRIWRIRAGET 1132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,828,750 Number of extensions: 173305 Number of successful extensions: 475 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 463 Number of HSP's successfully gapped: 1 Length of query: 1119 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 1024 Effective length of database: 1,170,045 Effective search space: 1198126080 Effective search space used: 1198126080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 136 (57.0 bits)