BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0034.p (599 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 960 0.0 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 872 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 87 5e-18 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 960 bits (2482), Expect = 0.0, Method: Compositional matrix adjust. Identities = 463/593 (78%), Positives = 510/593 (86%), Gaps = 9/593 (1%) Query: 1 VIGDFNQWDGRRHPMRLRQSAGVWELFIPRLRDGTLYKYEIIGPDGRLQPSKADPMARXL 60 VIGDFNQWDGRR+PMRLRQSAGVWELF+PRL DG LYKYEIIGP G L P KADPMAR Sbjct: 233 VIGDFNQWDGRRNPMRLRQSAGVWELFVPRLGDGALYKYEIIGPHGELLPGKADPMAR-- 290 Query: 61 RATELPPATASRVIDDR---DGWRWRDDEWMAQRAQKQSLQAPISIYEVHAASWIRVLEE 117 ATE+PPATASRVIDD G RW+D W+ QRAQ Q+ QAP+SIYEVHAASW+R+ E Sbjct: 291 -ATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQTRQAPMSIYEVHAASWLRLPGE 349 Query: 118 EGRSLTWNELAERLIPYAKGMGFTHLELLPVMEHPFGGSWGYQPLSQFAPSARFGSAADF 177 R+L WNELA+RLIPY KGMGFTH+ELLPVMEHPFGGSWGYQPLSQFAPSARFG A+F Sbjct: 350 GNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGSWGYQPLSQFAPSARFGPPAEF 409 Query: 178 AGFVDSCHREGLGVILDWVPAHFPSDGHGLAYFDGTPLYEHSDPREGFHQDWNTLIYNLG 237 A FVD+CHR G+GV+LDWVPAHFPSD HGLAYFDGTPLYEH DPREG HQDWNT IYNLG Sbjct: 410 ATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLYEHGDPREGIHQDWNTHIYNLG 469 Query: 238 RNEVRGFMIASALEWLEHFHVDGLRVDAVASMLYRDYSREPGQWVPNIHGGRENLESVDF 297 R+EVRGF+IASALEWLEHFHVDGLRVDAVASMLYRDYSR GQWVPNIHGGRENLE+VDF Sbjct: 470 RHEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRSSGQWVPNIHGGRENLEAVDF 529 Query: 298 LRELNQTVLERCPGALMIAEESTSWAGVTAPVKEGGLGFTYKWNMGWMHDTLRYMEEDSI 357 LRELNQTV ERCPGALMIAEEST+W GVTAPV EGGLGFTYKWNMGWMHDTLRY+E DSI Sbjct: 530 LRELNQTVAERCPGALMIAEESTAWPGVTAPVSEGGLGFTYKWNMGWMHDTLRYVELDSI 589 Query: 358 YRRYRHHDFTFGMIYAYSEAFVLPISHDEVVHGKRTLLGKMPGDYWQKLANLRAYLGFMW 417 YRRY HHD TFGMIYA+SE+FVLPISHDEVVHGK TLL KM GD WQ+ ANLRAYLGFMW Sbjct: 590 YRRYHHHDITFGMIYAFSESFVLPISHDEVVHGKGTLLQKMAGDAWQRFANLRAYLGFMW 649 Query: 418 THPGKKLLFMGCEFGQYQEFDHDKSPQWDLLDDPRHRSIQRLVRDLNGLYTSLPALHLND 477 THPGKKLLFMGCEF + +EFD D+SP WDLLDD HR +QRLVRDLN LY PAL+ +D Sbjct: 650 THPGKKLLFMGCEFAETREFDPDRSPHWDLLDDAHHRGVQRLVRDLNRLYAGTPALYRHD 709 Query: 478 TRAEGFSWVVGNDNVNSVFAYFRHGNEGEGDEPLLVAVNMTPVPRHNYRIGVPVGAANEF 537 T A+GFSWVVGND +NSVFAY R ++ + P+LV +NMTPVPRH+YRIGVP G + Sbjct: 710 TEAKGFSWVVGNDELNSVFAYLRQTDD---EAPVLVVINMTPVPRHHYRIGVPPGQTQQD 766 Query: 538 QWKEILNTDAAIYGGSNLGNAGSVTAQPQEWHGHPASLLLTLPPLSTIILRRG 590 +W+EILNTDA YGGS+LGN G + AQ Q HGH +SL+LTLPPLST++LRRG Sbjct: 767 RWEEILNTDADCYGGSHLGNGGQLRAQAQACHGHASSLVLTLPPLSTLVLRRG 819 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 872 bits (2252), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/599 (71%), Positives = 488/599 (81%), Gaps = 17/599 (2%) Query: 1 VIGDFNQWDGRRHPMRLRQSAGVWELFIPRLRDGTLYKYEIIGPDGRLQPSKADPMARXL 60 V+GDFNQWDGRRH MRLR GVWELFIPR+ G +YKYEI G G L P KADP+AR Sbjct: 211 VVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKYEIRGRGGVLLPLKADPVAR-- 268 Query: 61 RATELPPATASRVIDDRDGWRWRDDEWMAQRAQKQSLQAPISIYEVHAASWIRVLEEEGR 120 ATE PPATAS V D R + W+D +WMA+RAQ+QSL AP+SIYEVH ASW RV E++ R Sbjct: 269 -ATEPPPATASVVADARP-YVWQDQQWMAERAQRQSLSAPMSIYEVHPASWRRVPEQDHR 326 Query: 121 SLTWNELAERLIPYAKGMGFTHLELLPVMEHPFGGSWGYQPLSQFAPSARFGSAADFAGF 180 +L W+ELA++LIPY GMGFTH+EL+PVMEHPFGGSWGYQP+S FAPSARFG FA F Sbjct: 327 NLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAF 386 Query: 181 VDSCHREGLGVILDWVPAHFPSDGHGLAYFDGTPLYEHSDPREGFHQDWNTLIYNLGRNE 240 VD CH+ G+GV+LDWVPAHFPSD HGLA FDGT LYEH+DPREGFHQDWNTLI+NLGRNE Sbjct: 387 VDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREGFHQDWNTLIFNLGRNE 446 Query: 241 VRGFMIASALEWLEHFHVDGLRVDAVASMLYRDYSREPGQWVPNIHGGRENLESVDFLRE 300 VRGF+IASALEWLEHFHVDGLRVDAVASMLYRDYSR+ G+WVPNIHGGRENLE+V FLRE Sbjct: 447 VRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKEGEWVPNIHGGRENLEAVAFLRE 506 Query: 301 LNQTVLERCPGALMIAEESTSWAGVTAPVKEG----GLGFTYKWNMGWMHDTLRYMEEDS 356 LN V +RCPGALMIAEESTSW GVTAPVKE GLGF+YKWNMGWM+DTLRYM+ D Sbjct: 507 LNTVVAQRCPGALMIAEESTSWPGVTAPVKEEGDHHGLGFSYKWNMGWMNDTLRYMQHDP 566 Query: 357 IYRRYRHHDFTFGMIYAYSEAFVLPISHDEVVHGKRTLLGKMPGDYWQKLANLRAYLGFM 416 ++RRY HHD TFG++Y +SE F+LPISHDEVVHGKR LL KMPGD W++ AN+RAY GFM Sbjct: 567 LFRRYHHHDMTFGLLYGFSENFILPISHDEVVHGKRALLNKMPGDQWRRFANVRAYFGFM 626 Query: 417 WTHPGKKLLFMGCEFGQYQEFDHDKSPQWDLLDD-----PRHRSIQRLVRDLNGLYTSLP 471 WTHPGKKLLFMG EFGQ EFDHDKSP W LLD+ HR +++LV DLN LY S P Sbjct: 627 WTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDEHAGHPAEHRQLRQLVGDLNRLYCSEP 686 Query: 472 ALHLNDTRAEGFSWVVGNDNVNSVFAYFRHGNEGEGDEPLLVAVNMTPVPRHNYRIGVP- 530 ALH D RA+GF+W VG+D+VNSV AYFR+ +EG P+LV VN+TPVPR NYRIGVP Sbjct: 687 ALHQRDCRADGFAWAVGDDSVNSVLAYFRYADEG---PPVLVVVNLTPVPRVNYRIGVPS 743 Query: 531 VGAANEFQWKEILNTDAAIYGGSNLGNAGSVTAQPQEWHGHPASLLLTLPPLSTIILRR 589 + + W+E+LNTDA +YGGSN GN G+V Q WHGH AS+ LTLPPLST+ILRR Sbjct: 744 IAPGHHGVWREVLNTDAGLYGGSNNGNLGAVQVQELAWHGHAASIELTLPPLSTLILRR 802 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 86.7 bits (213), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 73/264 (27%), Positives = 116/264 (43%), Gaps = 43/264 (16%) Query: 35 TLYKYEIIGPDGRLQPSKADPMARXLRATELPPATASRVIDDRDGWRWRDDEWMAQRAQK 94 T+ + E++ PD P +R P+ ++ D + W+ +W + ++ Sbjct: 71 TISQGELVAPD---------PASRGQAGDVFDPS----LVVDAQAYVWQYPQWRGRPWEE 117 Query: 95 QSLQAPISIYEVHAASWIRVLEEEGRSLTWNELAERLIPYAKGMGFTHLELLPVMEHPFG 154 L YE+H G + + +RL A+ +G T +EL+PV E P Sbjct: 118 TVL------YELH----------PGAMGGFAGIRQRLAELAE-LGITAIELMPVAEFPGA 160 Query: 155 GSWGYQPLSQFAPSARFGSAADFAGFVDSCHREGLGVILDWVPAHFPSDGHGLAYFDGTP 214 +WGY + FAP A +G+ +D+ H G+ V LD V HF DG+ Sbjct: 161 HNWGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGN--------- 211 Query: 215 LYEHSDPREGFHQDWNTL---IYNLGRNEVRGFMIASALEWLEHFHVDGLRVDAVASMLY 271 Y H+ E F QD TL + R EV F +AL WL+ + DGLR+DAV ++ Sbjct: 212 -YLHAYAPEFFRQDMQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFDGLRLDAVHAICE 270 Query: 272 RDYSREPGQWVPNIHGGRENLESV 295 + GQ + GG+ ++ V Sbjct: 271 PAWLERLGQRIRTEIGGQRHVHLV 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,138,704 Number of extensions: 97309 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 3 Length of query: 599 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 508 Effective length of database: 1,188,985 Effective search space: 604004380 Effective search space used: 604004380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)