BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0031.p (770 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 751 0.0 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 98 2e-21 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 86 1e-17 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 81 3e-16 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 751 bits (1939), Expect = 0.0, Method: Compositional matrix adjust. Identities = 394/590 (66%), Positives = 454/590 (76%), Gaps = 26/590 (4%) Query: 13 NVRFHYRLPFGAEYLGNGRTSFRLWAPLAKTASVLIDGQRKVPMAAVDEGWFEVETDCEA 72 + RF + LPFGA+ GR FR+WAP A++ +V I G+ ++PM A +GWF +ET C+ Sbjct: 2 SARFSWNLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPMDAEPQGWFALETACDP 61 Query: 73 RSQYRYVLMSQTNGEISVPDPASRAQAGDVHADSVVIDASAYEWQTLDWRGRPWHETVLY 132 + YRYV + + GE+ PDPASR QAGDV S+V+DA AY WQ WRGRPW ETVLY Sbjct: 62 GATYRYVFDTISQGELVAPDPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEETVLY 121 Query: 133 ELHVGALGGFAGVLQRLPELAELGITAVELMPVAEFPGAYNWGYDGVLPYAPDASYGTPE 192 ELH GA+GGFAG+ QRL ELAELGITA+ELMPVAEFPGA+NWGYDGVLP+APDASYGTPE Sbjct: 122 ELHPGAMGGFAGIRQRLAELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASYGTPE 181 Query: 193 QLKQLIDTAHGLGMMVFLDVVYNHFGPDGNYLNSYAAPFFRTDSTTPWGETIDFRRPEVA 252 QLK LIDTAHGLGMMVFLDVVYNHFGPDGNYL++YA FFR D T WG TIDFRRPEV Sbjct: 182 QLKALIDTAHGLGMMVFLDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRRPEVD 241 Query: 253 DFFARNALYWLQEYRFDGLRLDAVHAISEQGWLRELARKVRRAIEQEDADIGLSRRHVHL 312 DFF +NALYWLQEYRFDGLRLDAVHAI E WL L +++R +IG +RHVHL Sbjct: 242 DFFTQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIR-------TEIG-GQRHVHL 293 Query: 313 VLEHDGNAASLLDGGTGKPGDGDK----YDAQWNDDGHHVLHVLLTGETGGYYSDYAVQP 368 VLEHDGNAA LL G G P D + YDAQWNDD HHVLHVLLTGE GYY+ YA +P Sbjct: 294 VLEHDGNAAHLL--GHGHPPDDGRSSHVYDAQWNDDAHHVLHVLLTGEDSGYYAAYAEEP 351 Query: 369 AEKLARCLREGFIYQGEPSPYRDNEVRGESSVELPPTAFVLFLQNHDQIGNRAQGERLTA 428 A KLARCL++GF+YQGE SPY E RGE S +LPPTAFV FLQNHDQIGNRA GERLT Sbjct: 352 AAKLARCLQQGFVYQGEVSPY-SGEPRGEPSADLPPTAFVNFLQNHDQIGNRAFGERLTV 410 Query: 429 LAHPDALRAAQALLLLSPQIPMLFMGEEYGASQPFYYFTSHADEKLADAVRNGRRNEFSR 488 LAHP AL AAQAL+LL+PQIPMLFMGEE+ A QPF YFTSH DE L AVR GRR EF+R Sbjct: 411 LAHPLALHAAQALVLLAPQIPMLFMGEEFCAVQPFLYFTSHRDEALVKAVREGRRREFAR 470 Query: 489 FPEFSDPEALDALPDPNSFSTFMASIPEPVSPDEDKSAGQWMGWCYR----LLLIRREAI 544 FP+F+DP L+ +PDPNSF+TF++SIPEP P +A C R LL IR+ I Sbjct: 471 FPQFADPALLEQIPDPNSFATFVSSIPEP-GPAPGSAA------CLRRVAELLHIRKLHI 523 Query: 545 TPYLPGAVALDAQAIGLKAVYARWRLNNGAVLNITVNLGEQPLRESLETL 594 PYL GA AL+A+AIG KAV+ARWRL+NG+VLNITVNL EQ ++ESL+ L Sbjct: 524 APYLRGARALEARAIGPKAVHARWRLSNGSVLNITVNLAEQAVQESLDQL 573 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 98.2 bits (243), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 89/291 (30%), Positives = 130/291 (44%), Gaps = 39/291 (13%) Query: 32 TSFRLWAPLAKTASVLID-----GQRKVPMAAVDEGWFEVETD-CEARSQYRYVLMSQTN 85 T F +WAP A+ SV+ D G+R V + G +E+ Y+Y + + Sbjct: 197 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKYEI--RGR 254 Query: 86 GEISVP---DPASRAQAGDVHADSVVIDASAYEWQTLDWRG-RPWHETV-----LYELHV 136 G + +P DP +RA SVV DA Y WQ W R +++ +YE+H Sbjct: 255 GGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQRQSLSAPMSIYEVHP 314 Query: 137 GA-----------LGGFAGVLQRLPELAELGITAVELMPVAEFPGAYNWGYDGVLPYAPD 185 + L Q +P + +G T +ELMPV E P +WGY + +AP Sbjct: 315 ASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFAPS 374 Query: 186 ASYGTPEQLKQLIDTAHGLGMMVFLDVVYNHFGP--------DGNYLNSYAAPFFRTDST 237 A +G PE+ +D H G+ V LD V HF DG L +A P R Sbjct: 375 ARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADP--REGFH 432 Query: 238 TPWGETI-DFRRPEVADFFARNALYWLQEYRFDGLRLDAVHAISEQGWLRE 287 W I + R EV F +AL WL+ + DGLR+DAV ++ + + R+ Sbjct: 433 QDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRK 483 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 85.9 bits (211), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 83/282 (29%), Positives = 120/282 (42%), Gaps = 37/282 (13%) Query: 34 FRLWAPLAKTASVLIDGQ----RKVPMAAVDEG--WFEVETDCEARSQYRYVLMSQTNGE 87 F +WAP A+ SV+ D R+ PM W + Y+Y ++ +GE Sbjct: 221 FAVWAPNAQRVSVIGDFNQWDGRRNPMRLRQSAGVWELFVPRLGDGALYKYEIIG-PHGE 279 Query: 88 I--SVPDPASRAQAGDVHADSVVID----ASAYEWQTLDW-RGRPWHET-----VLYELH 135 + DP +RA S VID S WQ W + R +T +YE+H Sbjct: 280 LLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQTRQAPMSIYEVH 339 Query: 136 VGALGGFAGVLQR-----------LPELAELGITAVELMPVAEFPGAYNWGYDGVLPYAP 184 + G R +P + +G T VEL+PV E P +WGY + +AP Sbjct: 340 AASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGSWGYQPLSQFAP 399 Query: 185 DASYGTPEQLKQLIDTAHGLGMMVFLDVVYNHFGPDGNYLNSY-AAPFF-----RTDSTT 238 A +G P + +D H G+ V LD V HF D + L + P + R Sbjct: 400 SARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLYEHGDPREGIHQ 459 Query: 239 PWGETI-DFRRPEVADFFARNALYWLQEYRFDGLRLDAVHAI 279 W I + R EV F +AL WL+ + DGLR+DAV ++ Sbjct: 460 DWNTHIYNLGRHEVRGFLIASALEWLEHFHVDGLRVDAVASM 501 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 81.3 bits (199), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 61/207 (29%), Positives = 89/207 (42%), Gaps = 40/207 (19%) Query: 108 VIDASAYEWQTLDWRGRPWHETVLYELHVGAL------------GGFAGVLQ--RLPELA 153 ++ A+ W+ PW++TV+YE HV + G FA + + L Sbjct: 144 MVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLREDLLPHERGTFAALADPAFIDHLL 203 Query: 154 ELGITAVELMPVAEFP--------GAYN-WGYDGVLPYAPDASY---GTPEQLKQLIDTA 201 LG+T +ELMPV F G +N WGY + +AP+ Y G P +L+ + Sbjct: 204 RLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAVRRL 263 Query: 202 HGLGMMVFLDVVYNH------FGPDGNYLNSYAAPFFRT--------DSTTPWGETIDFR 247 H G+ V LDVVYNH GP ++ A +FR + T G T++ Sbjct: 264 HSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTLNLS 323 Query: 248 RPEVADFFARNALYWLQEYRFDGLRLD 274 P V + YW Q + DG R D Sbjct: 324 HPRVLQMVLDSLRYWAQSFHIDGFRFD 350 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,424,851 Number of extensions: 103199 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 4 Length of query: 770 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 677 Effective length of database: 1,179,515 Effective search space: 798531655 Effective search space used: 798531655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)