BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0022.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 626 e-180 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 76 5e-15 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 55 8e-09 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 626 bits (1614), Expect = e-180, Method: Compositional matrix adjust. Identities = 309/456 (67%), Positives = 364/456 (79%), Gaps = 18/456 (3%) Query: 1 LQENNPDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGT 60 LQEN+P+LLFRDVAAY+Q I P QA V HQAIAQAY QRGVAH++IPADVIG K G Sbjct: 112 LQENSPELLFRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSG- 170 Query: 61 PSVASLNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGARSSIADIAVLAEKLQAP 120 SV SL+TLR + ++PP ++I AA LINAA SVAIF GNG R +I ++ LA KLQAP Sbjct: 171 -SVPSLHTLRERTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAP 229 Query: 121 ILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLMLGTDYPYSEFLPTRTQTV 180 ++HTFR KD+ YDHPHWIGGVGLIGGAPG DA+ A ++LMLG+DYPYSEFLPT+++ + Sbjct: 230 VMHTFRGKDLASYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVI 289 Query: 181 QIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQGKTDNAFLRKVGEHRKQWNETLDKH 240 QIDERGFVLGRR PV LGITGSV PAV QLL +V+GKTD AFL++ HR++WN LD Sbjct: 290 QIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSA 349 Query: 241 AVLPADAGKGPVKPQYLARRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQRILASFNN 300 A + K +KPQ +AR +SD A +DA FVVDTGVVTLWCGNWIRQ G QRILASFNN Sbjct: 350 AEIKRHHKK--IKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNN 407 Query: 301 AAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVVFNNREWGL 360 AAVGTSLGQ NGIQALDR+RQV+VAVGDGGFTMLLGEFMT+VEH+LPVKVVVFNNREWGL Sbjct: 408 AAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGL 467 Query: 361 VHLEMEEAGLPAFE--------------KXGAQGFTAKTPAQLEEVLEPFLAAPGPAILN 406 VHLEMEEAG+PAFE G QGF+ +TP +LE L+ LAAPGPA+L+ Sbjct: 468 VHLEMEEAGMPAFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLD 527 Query: 407 VFIDPSELPIMPHIKLDQIWHFGMAKVKEAMISMGG 442 VFIDP ELP+MPHIK++QIW FG+AKV+E++I+M G Sbjct: 528 VFIDPDELPVMPHIKMEQIWRFGLAKVRESLIAMKG 563 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 76.3 bits (186), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 84/403 (20%), Positives = 154/403 (38%), Gaps = 33/403 (8%) Query: 46 ISIPADV-IGVKLPGTPSVASLNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGA- 103 + IP D+ + P + + +P + +I A QL+ +A+ I+ G G Sbjct: 161 VDIPKDITMHTCAYAYPKAVEMRSYKPVDK--GHSGQIRKALQLLLSAERPMIYTGGGII 218 Query: 104 -RSSIADIAVLAEKLQAPILHTFRAKDMLPYDHPHWIGGVGLIGGAPGMDAMRDAGLLLM 162 ++ ++ L ++L P +T ++G G+ G AM+ +L+ Sbjct: 219 LANAAPELNKLVDRLGYPCTNTLMGLGAYRSSSDKFVGMPGMHGTYEANMAMQHCDVLIA 278 Query: 163 LGTDYPY------SEFLPTRTQTVQIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQG 216 +G + F + + ID + +R+ VD+ I G+V + +LL Q+ Sbjct: 279 IGARFDDRVIGNPKHFASHARKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQL-- 336 Query: 217 KTDNAFLRKVGEHRKQWNETLD---KHAVLPADAGKGPVKPQYLARRLSDRAAEDAVFVV 273 D A R+ G W + ++ K L + +KPQ + +++ + DA Sbjct: 337 --DAAETRQNGAALDAWWKQINEWRKRDCLKFATSEEFIKPQSVVQKVWEVTKGDAFITS 394 Query: 274 DTGVVTLWCGNWIRQSGRQRILASFNNAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTM 333 D G +W + +R + S +G L G+Q + D V G+ M Sbjct: 395 DVGQHQMWAAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQMANPDATVACITGEASIQM 454 Query: 334 LLGEFMTAVEHKLPVKVVVFNNREWGLV-----------HLEMEEAGLPAF----EKXGA 378 + E T ++ L K+++ NNR G+V + E LP F E G Sbjct: 455 CIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGH 514 Query: 379 QGFTAKTPAQLEEVLEPFLAAPGPAILNVFIDPSELPIMPHIK 421 G + P ++ L A + FI + P +K Sbjct: 515 VGMKIEKPGDVDGALREAFAMKDRLVFMNFITDQTENVWPMVK 557 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 55.5 bits (132), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 91/445 (20%), Positives = 173/445 (38%), Gaps = 60/445 (13%) Query: 6 PDLLFRDVAAYTQTIIDPAQAVQVAHQAIAQAYHQRGVAHISIPADVIGVKLPGTPSVAS 65 P+ L + Q + DPAQ G +++P DV + S Sbjct: 166 PEQLLTALPRAIQVLTDPAQC---------------GPVTLALPQDVQTMAYDYPLDFFS 210 Query: 66 LNTLRPQPEVTPPESEIAHAAQLINAAKSVAIFVGNGAR--SSIADIAVLAEKLQAPILH 123 + P+ + P E+ A L+ A+ + G G ++ + AE+ P+ Sbjct: 211 PRVIVPRAQ-APAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRFAEQHGVPVAE 269 Query: 124 TFRAKDMLPYDHPHWIGGVGLIGGAPGMDAM-RDAGLLLMLGT---DY---PYSEFLPTR 176 T K LP+ H +G +G + G+P +A+ ++A +++ +GT D+ ++ F R Sbjct: 270 TQAGKSALPWSHALQMGAIG-VTGSPAANALAQEADVVIAVGTRLQDFTTGSHTLFAQAR 328 Query: 177 TQTVQIDERGFVLGRRMPVDLGITGSVGPAVTQLLPQVQG-KTDNAFLRKVGEHRKQWNE 235 + ++ R L + G + L ++G ++ + + + W E Sbjct: 329 LLNLNVNAMDAHKWR----GLALQADAGLGLEALSHALEGWRSAPEWHARAHTLAQDWRE 384 Query: 236 TLDKHAVLPADAGKGPVKPQYLA---RRLSDRAAEDAVFVVDTGVVTLWCGNWIRQSGRQ 292 + + G+ P + + R ++D ++D + V G + R Sbjct: 385 RVAQITGQTDTGGRLPYDGEVIGAIQRSVADSPSQD-IVVCAAGTLPAELHKLWRAGRPG 443 Query: 293 RILASFNNAAVGTSLGQGNGIQALDRDRQVIVAVGDGGFTMLLGEFMTAVEHKLPVKVVV 352 + + +G + G G++ R+VIV VGDG + M+ E T+V + VVV Sbjct: 444 AYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSVMLGAKLIVVV 503 Query: 353 FNNREWGLVHLEMEEA------------------GLPAFE------KXGAQGFTAKTPAQ 388 +NR +G ++ +++A G PA + GA G KT + Sbjct: 504 LDNRGYGCIN-RLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGALGENVKTITE 562 Query: 389 LEEVLEPFLAAPGPAILNVFIDPSE 413 LE L+ AA ++ + D S Sbjct: 563 LEAALQRARAADRSYLVCIDTDASR 587 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,437,304 Number of extensions: 62844 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 3 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)