BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0014.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [He... 303 1e-83 gi|300311788|ref|YP_003775880.1| methyl-accepting chemotaxis pro... 178 7e-46 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 94 2e-20 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 93 3e-20 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 91 2e-19 >gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 808 Score = 303 bits (777), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 143/202 (70%), Positives = 171/202 (84%) Query: 172 GTEQVADILASVTDSLPALASELGKAAPAVAIEDNGYVIHTHASPLLKNVFMHLIRNAMD 231 GTE + ++LA VTDSLPALA++LGKAAP V IEDNGYV+H+ A+PLL+NVFMHLIRNAMD Sbjct: 583 GTETIQEVLAPVTDSLPALAADLGKAAPVVQIEDNGYVVHSFAAPLLQNVFMHLIRNAMD 642 Query: 232 HGLETPEERQAKGKQAAGTIRLQLGVMDNMLHMALSDDGRGLALAHIRRTAVDKQLIAAD 291 HGLE+ EER AKGK AG I+L++GVM++M MALSDDGRGLAL +R+ ++K LI D Sbjct: 643 HGLESSEERLAKGKPEAGVIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVD 702 Query: 292 AVPSDAELARLIMRAGFSTRSEVTDVSGRGVGMDAVLDFVTQEHGRIEIEFLDHAEGADF 351 SDAE+ARLI++ GFSTRSEVT+VSGRGVGMDAVLDFVT+EHG+IEI F D EGA F Sbjct: 703 QALSDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPF 762 Query: 352 RPFQINVLLPENIAVEIDGIDV 373 R FQI V+LPEN+AVE+DGIDV Sbjct: 763 RSFQIIVMLPENVAVEVDGIDV 784 >gi|300311788|ref|YP_003775880.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 178 bits (452), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 88/162 (54%), Positives = 119/162 (73%) Query: 13 MLNQILFVLLGALLSGVPLALMLKRQKMRMRRMVDVDQVDALKRNVQLTLDEYQQNIDAR 72 M+N IL V LGA+L+G+PLAL+ RQ+ R +R++D Q++ +++ Q L +Q + A Sbjct: 1 MVNAILLVALGAVLAGLPLALLWLRQQRRAQRLIDAGQLELVRQQAQEALARQEQQMQAH 60 Query: 73 QQELEAQRRDNDVRLAQTRRDTEKVVAQLSSQNSQSVARVLENCDASQETISNLLGLMRT 132 +Q+ +R+ L + R ++E ++ QL SQ S + +RV ENCDASQ+TI+ LLGL+RT Sbjct: 61 EQQARQERQSAQQLLEEMRLESETMIGQLQSQQSDNFSRVKENCDASQDTIAKLLGLVRT 120 Query: 133 FERWHDDMNVLITHNREMHRKNDEFALIVNQVIIVALNAGTE 174 FERWHDDMNVLI HNR+MHR NDEFALIVNQVIIVALNA E Sbjct: 121 FERWHDDMNVLIAHNRDMHRMNDEFALIVNQVIIVALNASIE 162 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 94.0 bits (232), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 63/183 (34%), Positives = 102/183 (55%), Gaps = 17/183 (9%) Query: 190 LASELGKAAPAVAI----EDNGYVIHTHASPLLKNVFMHLIRNAMDHGLETPEERQAKGK 245 ++ +LGK V + E + +I A PL+ H++RN++DHGLE PE+R+A GK Sbjct: 535 ISRKLGKEVQLVLVGEETEADKNIIEALADPLI-----HIVRNSLDHGLEGPEQRRASGK 589 Query: 246 QAAGTIRLQLGVMDNMLHMALSDDGRGLALAHIRRTAVDKQLIAADAVP--SDAELARLI 303 A G++ + + + + + DDG+G+ A I+R A +K LI + SD + L+ Sbjct: 590 PALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQDAVNLV 649 Query: 304 MRAGFSTRSEVTDVSGRGVGMDAVLDFVTQEHGRIEIEFLDHAEGADFRPFQINVLLPEN 363 +GFST V+D+SGRGVGMD V + V + +G + + H+ QI+ LP + Sbjct: 650 FSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLAL----HSVSGKGTRLQIS--LPLS 703 Query: 364 IAV 366 +AV Sbjct: 704 MAV 706 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 51/124 (41%), Positives = 73/124 (58%) Query: 218 LKNVFMHLIRNAMDHGLETPEERQAKGKQAAGTIRLQLGVMDNMLHMALSDDGRGLALAH 277 L + MH++RNA+DHG+E ER A GK A +++L + + + DDGRG+ Sbjct: 437 LADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREA 496 Query: 278 IRRTAVDKQLIAADAVPSDAELARLIMRAGFSTRSEVTDVSGRGVGMDAVLDFVTQEHGR 337 I AV + L + DA SDAE+ R + GFST +VT++SGRGVGMD V + HG Sbjct: 497 ILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGE 556 Query: 338 IEIE 341 + I+ Sbjct: 557 VSID 560 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 58/145 (40%), Positives = 79/145 (54%), Gaps = 16/145 (11%) Query: 190 LASELGKAAPAVAIEDNGYVIHTHASPLLKNV-------FMHLIRNAMDHGLETPEERQA 242 LA +LGK +V H A+ L K + HL+RN++DHG+E P+ R+A Sbjct: 386 LAHKLGKQVE--------FVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARRA 437 Query: 243 KGKQAAGTIRLQLGVMDNMLHMALSDDGRGLALAHIRRTAVDKQLIAADAVPSDAELARL 302 GK AGT+ L + + ++DDG GL I A + + D + SDAE+ +L Sbjct: 438 SGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQGMPVTDNL-SDAEVWQL 496 Query: 303 IMRAGFSTRSEVTDVSGRGVGMDAV 327 I GFST +VTDVSGRGVGMD V Sbjct: 497 IFEPGFSTAEQVTDVSGRGVGMDVV 521 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,047,175 Number of extensions: 40777 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 8 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)