BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0298.p (434 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 598 e-172 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 582 e-167 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 294 1e-80 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 274 1e-74 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 271 6e-74 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 598 bits (1542), Expect = e-172, Method: Compositional matrix adjust. Identities = 292/417 (70%), Positives = 348/417 (83%) Query: 11 TVSSPPSKIGAVLRVTSGNFLEQFDFFLFGFYAHYISKVFFPVDSEFASLMLTFAVFGAG 70 T ++ SK VLRVTSGNF+E FDFFLFGFYA +ISK FFP D+EFASLMLTF FGAG Sbjct: 3 TSTASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAG 62 Query: 71 FLMRPLGAIILGAYIDKVGRRKGLIVTLSIMASGTVLIACVPGYTSIGLLAPALVLIGRL 130 FLMRPLGAIILGAY+D+VGRR+GLIVTL++MA GT+LIA VP Y +IG LAP LVLIGRL Sbjct: 63 FLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRL 122 Query: 131 LQGFSAGAELGGVSVYLAEIATPGHKGFYTAWQSASQQVAIVMAAALGYGLNQLLLPAEL 190 LQGFSAG ELGGVSVYLAE+ATPG+KGFY +WQSASQQVAI++AAA+GY L+ L PAE+ Sbjct: 123 LQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEV 182 Query: 191 AAWGWRIPFLIGCVIVPFIFILRRSLQETEEFQARRHHPDIREVFDSMLKNWRTVLTGML 250 + WGWRIPF IGC+IVP +F++RRSLQETEEF R+H P ++F SM++NW+ V+ GM+ Sbjct: 183 SGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMM 242 Query: 251 FVAMTTTTFYLITVYTPTFGRTVLHLSAADSLLVTLLVGLSNFAWLPIGGALSDRIGRKP 310 V+MTT +FYLITVYTPTFG+ VL LS SL+VTL VGLSNF WLPI GALSDR+GR+P Sbjct: 243 LVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRP 302 Query: 311 ILVGISLLALLTAYPAMSWLVANPSFGHLLTVLLVFSFYFGMYNGAMVVALTEVMPINVR 370 +LV ++L +LTAYPA++WLV + SF +L V L SF + YNGAMVVALTEVMP +VR Sbjct: 303 LLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVR 362 Query: 371 VVGFSLAFSLSTAVFGGFTPAISTYLIDLTANKAAPGLWMSFAALCGLAATLILYRR 427 GFSLA+SL+TAVFGGFTPAI+T LI+ T +KAAPG+WMS AA+CGL ATLILYR Sbjct: 363 TAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRN 419 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 582 bits (1499), Expect = e-167, Method: Compositional matrix adjust. Identities = 290/421 (68%), Positives = 347/421 (82%) Query: 11 TVSSPPSKIGAVLRVTSGNFLEQFDFFLFGFYAHYISKVFFPVDSEFASLMLTFAVFGAG 70 T ++ SK+ VLRVTSGNFLE FDFFL+GFYA YISK FFP D+EF SL+LTF FGAG Sbjct: 5 TTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAG 64 Query: 71 FLMRPLGAIILGAYIDKVGRRKGLIVTLSIMASGTVLIACVPGYTSIGLLAPALVLIGRL 130 FLMRPLGAIILG+YID+VGRR+GLIVTL+IMA GT+LIA VPGY +IG+ AP LVL GRL Sbjct: 65 FLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRL 124 Query: 131 LQGFSAGAELGGVSVYLAEIATPGHKGFYTAWQSASQQVAIVMAAALGYGLNQLLLPAEL 190 LQGFSAG ELGGVSVYLAE+ATPG+KGFY +WQSASQQ AI+ +A +GY ++Q L P ++ Sbjct: 125 LQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQI 184 Query: 191 AAWGWRIPFLIGCVIVPFIFILRRSLQETEEFQARRHHPDIREVFDSMLKNWRTVLTGML 250 + WGWRIPF IGC+IVP IFI+RRSLQET+EF AR+ HP E+ S+ +NW+ V+ GM+ Sbjct: 185 SDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMM 244 Query: 251 FVAMTTTTFYLITVYTPTFGRTVLHLSAADSLLVTLLVGLSNFAWLPIGGALSDRIGRKP 310 V MTT +FYLITVYTPTFG+ VL LSA+DSL+VT VGLSNF WLPI GALSDR+GR+P Sbjct: 245 MVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRP 304 Query: 311 ILVGISLLALLTAYPAMSWLVANPSFGHLLTVLLVFSFYFGMYNGAMVVALTEVMPINVR 370 +LV +LL LLTAYP MSWLV++ SF +LL L SF + YNGA VVALTEVMP +VR Sbjct: 305 VLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVR 364 Query: 371 VVGFSLAFSLSTAVFGGFTPAISTYLIDLTANKAAPGLWMSFAALCGLAATLILYRRGGA 430 VGFSLA+SL+TA+FGGFTP +ST+LI+ + +KAAPG WMSFAA+CGL ATL+LYR A Sbjct: 365 TVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGKSA 424 Query: 431 A 431 A Sbjct: 425 A 425 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 294 bits (752), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 167/424 (39%), Positives = 253/424 (59%), Gaps = 8/424 (1%) Query: 1 MTAKTTTVGDTVSSPPSKIGAVLRVTSGNFLEQFDFFLFGFYAHYISKVFFPVDSEFASL 60 M++ T G ++ P ++ A++ + GN LE FD ++G +A I+K FFP + SL Sbjct: 1 MSSNTAAPGADITHPQAR-RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSL 59 Query: 61 MLTFAVFGAGFLMRPLGAIILGAYIDKVGRRKGLIVTLSIMASGTVLIACVPGYTSIGLL 120 +LTFA FG F MRPLGA++LGAY D+ GR+ L++++ +M GT +IA +P Y SIGLL Sbjct: 60 LLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLL 119 Query: 121 APALVLIGRLLQGFSAGAELGGVSVYLAEIATPGHKGFYTAWQSASQQVAIVMAAALGYG 180 APA + +G+++QGFSAG E G + +L E A P +GF+++WQ ASQ +++++AA G Sbjct: 120 APAGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALFGAV 178 Query: 181 LNQLLLPAELAAWGWRIPFLIGCVIVPFIFILRRSLQETEEFQ--ARRHHPDIREVFDSM 238 LN +L P +LA+WGWR+PF+ G +I P +RR L E EF+ + + + +R+ F Sbjct: 179 LNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAH- 237 Query: 239 LKNWRTVLTGMLFVAMTTTTFYLITVYTPTFGRTVLHLSAADSLLVTLLVGLSNFAWLPI 298 + R +L G V M T + YL ++Y PT+ L L A S + GL F P+ Sbjct: 238 -QKMR-LLIGAGSVIMATVSVYL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPL 294 Query: 299 GGALSDRIGRKPILVGISLLALLTAYPAMSWLVANPSFGHLLTVLLVFSFYFGMYNGAMV 358 GALSD+IGR P ++ S L ++ YP +L +P F LL + V MY AM Sbjct: 295 VGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMP 354 Query: 359 VALTEVMPINVRVVGFSLAFSLSTAVFGGFTPAISTYLIDLTANKAAPGLWMSFAALCGL 418 L ++ P+ R G SLA++++ +FGGF I T+LID T +K + ++ F A+ L Sbjct: 355 ALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSL 414 Query: 419 AATL 422 A+L Sbjct: 415 IASL 418 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 148/419 (35%), Positives = 235/419 (56%), Gaps = 2/419 (0%) Query: 17 SKIGAVLRVTSGNFLEQFDFFLFGFYAHYISKVFFPVDSEFASLMLTFAVFGAGFLMRPL 76 +K V+ GN LE +DF ++GF++ I+++FFP D+E+ SL++ A FG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 77 GAIILGAYIDKVGRRKGLIVTLSIMASGTVLIACVPGYTSIGLLAPALVLIGRLLQGFSA 136 G ++LG Y D+ GR+ + V + +M +IA P Y +IG+ AP ++++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 137 GAELGGVSVYLAEIATPGHKGFYTAWQSASQQVAIVMAAALGYGLNQLLLPAELAAWGWR 196 G E + +L E A +G Y +WQ Q +A+ A +G + + L P L +WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 197 IPFLIGCVIVPFIFILRRSLQETEEFQARRHHPDIR--EVFDSMLKNWRTVLTGMLFVAM 254 +PF++G +I P +RR + ET++F + P+ ++ D + N R VL M V Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 255 TTTTFYLITVYTPTFGRTVLHLSAADSLLVTLLVGLSNFAWLPIGGALSDRIGRKPILVG 314 T FY++ V PTF L L + +V ++ A +P GALSDR+GR+ +L+ Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 315 ISLLALLTAYPAMSWLVANPSFGHLLTVLLVFSFYFGMYNGAMVVALTEVMPINVRVVGF 374 SL LL YP W+ A PS LL + ++ G G M AL+E P VR G Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 375 SLAFSLSTAVFGGFTPAISTYLIDLTANKAAPGLWMSFAALCGLAATLILYRRGGAARD 433 ++A++L+ +FGGF P I T+L + + AP ++ FAA G++A + + AA++ Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRDQSPAAKN 424 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 271 bits (694), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 162/425 (38%), Positives = 238/425 (56%), Gaps = 8/425 (1%) Query: 1 MTAKTTTVGDTVS-SPPSKIGAVLRVTSGNFLEQFDFFLFGFYAHYISKVFFPVDSEFAS 59 M A T VS S + A++ GN LE FDF ++ F+A I+K+FFP ++ S Sbjct: 1 MQASTLPADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTS 60 Query: 60 LMLTFAVFGAGFLMRPLGAIILGAYIDKVGRRKGLIVTLSIMASGTVLIACVPGYTSIGL 119 L+L A FG GF MRP+G I+LG Y D+VGR+ L VT+ +MA GT +I P Y SIGL Sbjct: 61 LLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGL 120 Query: 120 LAPALVLIGRLLQGFSAGAELGGVSVYLAEIATPGHKGFYTAWQSASQQVAIVMAAALGY 179 AP ++++ RLLQGFSAG E+G + +L E A + +Y++W AS VA+++ AA+G Sbjct: 121 FAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGT 180 Query: 180 GLNQLLLPAELAAWGWRIPFLIGCVIVPFIFILRRSLQETEEFQARRHHPD--IREVFDS 237 + L A LA+WGWR+PFL+G +I P + +R L+ET F + D ++EV Sbjct: 181 FVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEV--- 237 Query: 238 MLKNW-RTVLTGMLFVAMTTTTFYLITVYTPTFGRTVLHLSAADSLLVTLLVGLSNFAWL 296 ++N+ R V + T Y++ Y PT+ VL L + ++ GL Sbjct: 238 -IRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLA 296 Query: 297 PIGGALSDRIGRKPILVGISLLALLTAYPAMSWLVANPSFGHLLTVLLVFSFYFGMYNGA 356 PI G L+DRIGR+P+L G + L L+ AYP +++ + PS LL LVF Y G Sbjct: 297 PIVGRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGP 356 Query: 357 MVVALTEVMPINVRVVGFSLAFSLSTAVFGGFTPAISTYLIDLTANKAAPGLWMSFAALC 416 ++ A +E+ P V G S+A++L+ +FGGF T+LI T + AP ++ AA Sbjct: 357 ILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAI 416 Query: 417 GLAAT 421 T Sbjct: 417 SFVGT 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,298,243 Number of extensions: 53047 Number of successful extensions: 385 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 364 Number of HSP's successfully gapped: 15 Length of query: 434 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 346 Effective length of database: 1,203,190 Effective search space: 416303740 Effective search space used: 416303740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)