BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0270.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 400 e-113 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 384 e-107 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 376 e-105 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 363 e-101 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 316 2e-87 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 400 bits (1029), Expect = e-113, Method: Compositional matrix adjust. Identities = 209/429 (48%), Positives = 285/429 (66%), Gaps = 6/429 (1%) Query: 6 EERVSRKLMLRIIPFVMLLYFISFLDRVNVGFAAITMNKDLGLSPTAFGLGGGLFFIGYF 65 E+ +K+M R+IPF++L Y +S+LDR+NVGFAA+TMNKDLGL+P+ FGLG GLFFIGYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 66 LFEVPSNLILHKVGARIWIARVMITWGIISAISAFVVGPNSFYLLRFLLGVAEAGFFPGI 125 FE+PSNL LH+ GAR+WI+R+MI+WG+IS +AFVVGP SF L RFLLG+AEAGF PGI Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 126 ILYLSLWFPAKQRAVAAAWFMAAAPISVALGSPLSGAIMQLPGLFGLKDWQMLYIIEAAP 185 LY + WFP R A A+F+ P++ +GSPLSGA+M+L G++G K WQ+L ++EA P Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 186 AVILGFVVLKYMTDKPEQAHWLAQDEREWLVARLKSEADAKKGGHGHTASAWSALKDPRV 245 AV+LG + L + D+P +A WL+ DE++WL L +E + HG+ A + RV Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLK--DAFTNWRV 257 Query: 246 LALALIYFGTSAGLYTLGLWVPLMIREFGFTPFETGLLTGVPSVVAVVAMVLWARHSDRS 305 ALA F G ++GLW+P +IREFG + GL+ +P +V VAM LWAR ++RS Sbjct: 258 FALAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 306 GERTWHVVIPCVVACVGFLLAGNATTALFVVLSLIVVNVGISSAKAPLWAMPSTFLSGAG 365 R + V V+A + ++ T L +L++ V I S +A WA+PS FL+G Sbjct: 318 ERRLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRA 377 Query: 366 AAAGIAMINSLGNLGGFVGPFAIGWLKNVTGGYAAGLYVVAASLALSAVVTLMLSRQTAQ 425 AA G+A+I S+GNLGGFVGP IG+++ T G+ L VA +L L AV+TL L Sbjct: 378 AAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLAL----GD 433 Query: 426 PDRVAKASH 434 P RV S Sbjct: 434 PSRVPPTSK 442 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 384 bits (985), Expect = e-107, Method: Compositional matrix adjust. Identities = 205/419 (48%), Positives = 274/419 (65%), Gaps = 12/419 (2%) Query: 6 EERVSRKLMLRIIPFVMLLYFISFLDRVNVGFAAITMNKDLGLSPTAFGLGGGLFFIGYF 65 E+ K+ R++P + L Y S+LDRVNVGFA + M +DL S T +GLG G+FF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 66 LFEVPSNLILHKVGARIWIARVMITWGIISAISAFVVGPNSFYLLRFLLGVAEAGFFPGI 125 +FEVPSN+ILHKVGAR+WIAR+MITWGIIS A+V P FY++RFLLGVAEAGFFPG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 126 ILYLSLWFPAKQRAVAAAWFMAAAPISVALGSPLSGAIMQ-LPGLFGLKDWQMLYIIEAA 184 ILYL+ W+PA +R A FM +S +G PLSG IM +PG++G WQ ++I+EA Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 185 PAVILGFVVLKYMTDKPEQAHWLAQDEREWLVARLKSEADAKKGGHGHTASAWSALKDPR 244 P++ILG +V+ YM D+ A WL+++E+ L A + E K+ S + + R Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE-----HLSLGAMFANGR 251 Query: 245 VLALALIYFGTSAGLYTLGLWVPLMIREFGFT-PFETGLLTGVPSVVAVVAMVLWARHSD 303 V ALIYF GLY + W+P +I+ G + P GLLT +P A AMVL R +D Sbjct: 252 VWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSAD 311 Query: 304 RSGERTWHVVIPCVVACVGFLLA---GNATTALFVVLSLIVVNVGISSAKAPLWAMPSTF 360 ER WHV IP + C+G LL+ G+ TT + SL + +VGI + W++P+ F Sbjct: 312 ARRERRWHVAIPAFIGCIGLLLSTQYGHNTT--LAMASLTLASVGILTTLPLFWSLPTAF 369 Query: 361 LSGAGAAAGIAMINSLGNLGGFVGPFAIGWLKNVTGGYAAGLYVVAASLALSAVVTLML 419 LSG AAAGIA+INSLGNL GFV PF +GWLK+ T AG+YV+AASL + ++TL L Sbjct: 370 LSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL 428 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 376 bits (966), Expect = e-105, Method: Compositional matrix adjust. Identities = 191/423 (45%), Positives = 267/423 (63%), Gaps = 3/423 (0%) Query: 2 KTEIEERVSRKLMLRIIPFVMLLYFISFLDRVNVGFAAITMNKDLGLSPTAFGLGGGLFF 61 + +E+ RK+ R++PF+ML Y +F+DR N+G AA+ MN DLGLSP FG LFF Sbjct: 7 EKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFF 66 Query: 62 IGYFLFEVPSNLILHKVGARIWIARVMITWGIISAISAFVVGPNSFYLLRFLLGVAEAGF 121 I YF+FEVPSNL L K GAR W+AR+MITWG+ISA +A V GPNS Y +RF+LG AEAGF Sbjct: 67 IAYFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGF 126 Query: 122 FPGIILYLSLWFPAKQRAVAAAWFMAAAPISVALGSPLSGAIMQLPGLFGLKDWQMLYII 181 FPG++LYL+ W P+ RA A FM A P + + SP+S ++Q+ G+ GL+ W L+++ Sbjct: 127 FPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFML 186 Query: 182 EAAPAVILGFVVLKYMTDKPEQAHWLAQDEREWLVARLKSEADAKKGGHGHTASAWSALK 241 E P V+LG V L ++TD+P+QA WL+ ++R+WL + EA A + H W Sbjct: 187 EGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEA-ASRAAKQHI-PLWKLFS 244 Query: 242 DPRVLALALIYFGTSAGLYTLGLWVPLMIREFGFTPFETGLLTGVPSVVAVVAMVLWARH 301 P V +AL+ SA L +W P +++ FG + +TGL+ +P ++A V MV W RH Sbjct: 245 QPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRH 304 Query: 302 SDRSGERTWHVVIPCVVACVGFLLAGNATTALFVVLSLIVVNVGISSAKAPLWAMPSTFL 361 SDR+GER WH IP ++ G LA A + VVL L +V +G S K P WAM S++L Sbjct: 305 SDRTGERRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMASSWL 364 Query: 362 SGAGAAAGIAMINSLGNL-GGFVGPFAIGWLKNVTGGYAAGLYVVAASLALSAVVTLMLS 420 + AAAG+A IN++ NL GG + GW+K+ TG YA L + +S + L LS Sbjct: 365 ATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLS 424 Query: 421 RQT 423 R++ Sbjct: 425 RKS 427 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 363 bits (932), Expect = e-101, Method: Compositional matrix adjust. Identities = 201/423 (47%), Positives = 278/423 (65%), Gaps = 13/423 (3%) Query: 1 MKTEIEERVSRKLMLRIIPFVMLLYFISFLDRVNVGFAAITMNKDLGLSPTAFGLGGGLF 60 + + ++ + +K+ RI+PF+ML Y I++LDRVNVGFA + M++DLG S T FGLG GLF Sbjct: 13 VNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLF 72 Query: 61 FIGYFLFEVPSNLILHKVGARIWIARVMITWGIISAISAFVVGPNSFYLLRFLLGVAEAG 120 FIGYFLFEVPSN+++HKVGARIWIAR+MITWGI+SA FV FY+LRFLLG+AEAG Sbjct: 73 FIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAG 132 Query: 121 FFPGIILYLSLWFPAKQRAVAAAWFMAAAPISVALGSPLSGAIMQ-LPGLFGLKDWQMLY 179 F+PGIILYL+ WFP+ +RA A FM+ P++ LG+PLSG IM GL+ WQ ++ Sbjct: 133 FYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMF 192 Query: 180 IIEAAPAVILGFVVLKYMTDKPEQAHWLAQDEREWLVARLKSEADAKKGGHGHTASAWSA 239 +IEA PAV++G + Y+ + + A WL +E+ L A + +A K+ HG A Sbjct: 193 LIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGA----I 248 Query: 240 LKDPRVLALALIYFGTSAGLYTLGLWVPLMIREFGFT-PFETGLLTGVPSVVAVVAMVLW 298 +KD RV + LIYF G Y L LW+P +++ G E GLL+ +P A++AM L Sbjct: 249 VKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLI 308 Query: 299 ARHSDRSGERTWHVVIPCVVACVGF----LLAGNATTALFVVLSLIVVNVGISSAKAPLW 354 R +DR ER WH+VIP ++ VGF L A N + + SL + G+ + W Sbjct: 309 GRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVS---IASLSLAAAGVLTCAPLFW 365 Query: 355 AMPSTFLSGAGAAAGIAMINSLGNLGGFVGPFAIGWLKNVTGGYAAGLYVVAASLALSAV 414 ++P+ FLSGA AA GIA INS+GNL GFV P+ IG+LK++T A G+Y++A L + ++ Sbjct: 366 SLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSI 425 Query: 415 VTL 417 TL Sbjct: 426 ATL 428 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 316 bits (810), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 180/422 (42%), Positives = 266/422 (63%), Gaps = 13/422 (3%) Query: 7 ERVSRKLMLRIIPFVMLLYFISFLDRVNVGFAAITMNKDLGLSPTAFGLGGGLFFIGYFL 66 E RK+ R+IPF++ L+ ++++DRVNVGFA + M +DL S +GLG G+FFIGYF+ Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 67 FEVPSNLILHKVGARIWIARVMITWGIISAISAFVVGPNSFYLLRFLLGVAEAGFFPGII 126 FEVPSNL+L K+GAR +AR+ I WG+ S A+V P SFY+LRFLLG+ EAGFFPG++ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 127 LYLSLWFPAKQRAVAAAWFMAAAPISVALGSPLSGAIMQ-LPGLFGLKDWQMLYIIEAAP 185 LYL+ WFPA++RA FM + I+ A+G P++G IM + G+ L +WQ L+I+E P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 186 AVILGFVVLKYMTDKPEQAHWLAQDEREWLVARLKSEADAKKGGHGHTASAWSALKDPRV 245 +++ GF VL ++ ++P A WL++ E+ + L +E A G S A + RV Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAP----GKHFSFKDACTNYRV 257 Query: 246 LALALIYFGTSAGLYTLGLWVPLMIREFGFT-PFETGLLTGVPSVVAVVAMVLWARHSDR 304 A +YF +G T+ W P +I+E G + GL++ +P + +AM+ HSD+ Sbjct: 258 WICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDK 317 Query: 305 SGERTWHVVIPCVVACVGF----LLAGNATTALFVVLSLIVVNVGISSAKAPLWAMPSTF 360 +GER H I ++AC+G + GNAT AL SL + +GI +A W++P F Sbjct: 318 TGERRLHSAIATLIACIGLVATGMTLGNATLAL---CSLTLAAIGILAAFPVFWSIPGAF 374 Query: 361 LSGAGAAAGIAMINSLGNLGGFVGPFAIGWLKNVTGGYAAGLYVVAASLALSAVVTLMLS 420 L+G AA GIA+INS+GNL GFV P+ IG LK TG ++GLY VAA +++ + ++ Sbjct: 375 LAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFI 434 Query: 421 RQ 422 R+ Sbjct: 435 RK 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,168,441 Number of extensions: 42689 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 270 Number of HSP's successfully gapped: 21 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)