BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0269.p (306 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 78 1e-15 gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 73 2e-14 gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro... 61 1e-10 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 73/300 (24%), Positives = 119/300 (39%), Gaps = 49/300 (16%) Query: 21 VNGFQMHYVDEGDRSREVVLCLHGEPTWGYLFRSLITALRSDYRVVVPDHMGFGKSETPQ 80 ++ H + GD +L LHG +G F L L YR + D G G+S+ Sbjct: 10 LSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDA 69 Query: 81 DRSYWLQDHIDNLEKLVLQLDLKEITLVMHDFGGPVGMGLAARHPGRIKR-IISTNGPTP 139 +Y+ ++ ++E LV +L+L++ L+ H GG + AARHP +++R II GP Sbjct: 70 GANYYTDAYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGA 129 Query: 140 FGHPDGMERLMENAAIAP--WFQW-----IVKANEDGSLEQVLGQLGFNILSTMKINGFE 192 F + G R+ A P + W ++A E+ Q N+L G+ Sbjct: 130 FENSPGATRIRRELATTPSHFASWDEASTFMRALRPSVTEEARQQRLHNMLKPAAQGGYT 189 Query: 193 ---NHGVISDTWISAYGAHFATPGDCLGAIGWAKGFATGAHQFEVPDAAAIATIRSQ--- 246 +H IS T +S PD + + +R Sbjct: 190 WRYDHVGISSTRLS-------------------------------PDLSRVVDLRPHIMA 218 Query: 247 ---PALAIWGDADKTLHPPIFLPLFTSLFPVADVRRLPEAGHYCFEDAPDEIAGIVRRFL 303 P L + G+ L + + L + +AGHY +D P+ A +VR+FL Sbjct: 219 LMCPTLVVRGERSDYLSAEMAAEM-RQLNARILTSTIADAGHYIHDDQPEAFAEVVRQFL 277 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 73/302 (24%), Positives = 117/302 (38%), Gaps = 31/302 (10%) Query: 18 FQEVNGFQMHYVDEGDRSREVVLCLHGEPTWGYLFRSLITALRSDYRVVVPDHMGFGKSE 77 F + G + H GD + LHG F+ ++ AL+ + V+ PD GFG +E Sbjct: 14 FLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWHVIAPDWRGFGLTE 73 Query: 78 TPQDRSYWLQDHIDNLEKLVLQLDLKE-ITLVMHDFGGPVGMGLAARHPGRIKRIISTNG 136 Q SYW D++ +L+ ++ E I L+ H GG V A P RIKR+I+ G Sbjct: 74 RSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLINLEG 133 Query: 137 PTPFGHPDGMERLMENAAIAPWFQWIVKANEDGSLEQVLGQLGFNILSTMKINGFENHGV 196 G+ A + QW+ + L G+ L + N+ Sbjct: 134 L-------GLPATQPEQAPGRFAQWMDEVRNRPVLR------GYATLEEVAQRLQRNNAR 180 Query: 197 ISDTWISAYGAHFATPGDCLGAIGWAKGFATGAHQFEVP----DAAAIATIRSQPALAIW 252 +SD + H+A D G + AH+ P +A R A +W Sbjct: 181 LSDERAAFLAQHWAQQDDS----GLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLW 236 Query: 253 GDADKTLHPPIFLPLF---------TSLFPVADVRRLPEAGHYCFEDAPDEIAGIVRRFL 303 +A + F P + P V + +AGH D P+++A ++ FL Sbjct: 237 IEAIDSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPEQLAQLIEAFL 296 Query: 304 KN 305 + Sbjct: 297 QQ 298 >gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 64/238 (26%), Positives = 96/238 (40%), Gaps = 35/238 (14%) Query: 26 MHYV----DEGDRSREVVLCLHGEPTWGYLFRSLITALRSDYRVVVPDHMGFGKSETPQD 81 +HY G R V+ H T ++ L L +D RV+ DH G G SE D Sbjct: 60 LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKA-D 118 Query: 82 RSYWLQDHIDNLEKLVLQLDLKEITLVMHDFGGPVGMGLAARHPGRIKRIISTNGPTPFG 141 Y + D D+ +L+ +LD + V GG VG LA RHPG ++ ++ N T Sbjct: 119 GLYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLAN--TTSA 176 Query: 142 HPDGMERLMENAAIAPWFQWIVKANEDGSLEQVLGQLGFNILSTMKINGFENHGVIS--D 199 +PD AA W Q I + G +++ + + + G S Sbjct: 177 YPD--------AAREAWQQRIATVRAE----------GIEVIADAVMGRYFHEGFRSAQA 218 Query: 200 TWISAYGAHFATPGDCLGAIGWAKGFATGAHQFEVPDAAAIATIRSQPALAIWGDADK 257 ++ Y T D +G +G T V AA + +IR P L I G+ D+ Sbjct: 219 ATVARYRQRLVTT-DAVGYVGCCHAVGT------VDTAARLGSIRV-PTLVIAGELDQ 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,134,997 Number of extensions: 51047 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 3 Length of query: 306 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 221 Effective length of database: 1,217,395 Effective search space: 269044295 Effective search space used: 269044295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)